Integration of Solid-State Nanopores in Microfluidic Networks via Transfer Printing of Suspended Membranes

Jain, Tarun; Guerrero, Ricardo; Aguilar, Carlos A.; Karnik, Rohit

doi:10.1021/ac302972c

Cited by 42 publications

(47 citation statements)

References 31 publications

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“…The graphene membrane (as prepared above) was mounted into a custom flow cell described by Jain and co-authors 32 . Membranes were first rinsed with ethanol to wet the nitride and graphene, followed by thorough rinsing in deionized water.…”

Section: Methodsmentioning

confidence: 99%

Heterogeneous sub-continuum ionic transport in statistically isolated graphene nanopores

et al. 2015

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Graphene and other two-dimensional materials offer a new class of ultrathin membranes that can have atomically defined nanopores with diameters approaching those of hydrated ions. These nanopores have the smallest possible pore volumes of any ion channel, which, due to ionic dehydration and electrokinetic effects, places them in a novel transport regime and allows membranes to be created that combine selective ionic transport with ultimate permeance and could lead to separations and sensing applications. However, experimental characterization and understanding of sub-continuum ionic transport in nanopores below 2 nm is limited. Here we show that isolated sub-2 nm pores in graphene exhibit, in contrast to larger pores, diverse transport behaviours consistent with ion transport over a free-energy barrier arising from ion dehydration and electrostatic interactions. Current-voltage measurements reveal that the conductance of graphene nanopores spans three orders of magnitude and that they display distinct linear, voltage-activated or rectified current-voltage characteristics and different cation-selectivity profiles. In rare cases, rapid, voltage-dependent stochastic switching is observed, consistent with the presence of a dissociable group in the pore vicinity. A modified Nernst-Planck model incorporating ion hydration and electrostatic effects quantitatively matches the observed behaviours.

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Section: Methodsmentioning

confidence: 99%

Heterogeneous sub-continuum ionic transport in statistically isolated graphene nanopores

et al. 2015

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“…Full realization of nanopore sequencing's potential will require additional progress in areas including nanopore parallelization 26 , channel setup 27–30 and microfluidics 31 .…”

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confidence: 99%

Decoding long nanopore sequencing reads of natural DNA

et al. 2014

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Nanopore sequencing of DNA is a single-molecule technique that may achieve long reads, low cost and high speed with minimal sample preparation and instrumentation. Here, we build on recent progress with respect to nanopore resolution and DNA control to interpret the procession of ion current levels observed during the translocation of DNA through the pore MspA. As approximately four nucleotides affect the ion current of each level, we measured the ion current corresponding to all 256 four-nucleotide combinations (quadromers). This quadromer map is highly predictive of ion current levels of previously unmeasured sequences derived from the bacteriophage phi X 174 genome. Furthermore, we show nanopore sequencing reads of phi X 174 up to 4,500 bases in length that can be unambiguously aligned to the phi X 174 reference genome, and demonstrate proof-of-concept utility with respect to hybrid genome assembly and polymorphism detection. This work provides the foundation for nanopore sequencing of long, complex, natural DNA strands.

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“…However, deposition of the stencil does not critically rely on transfer to raised platforms: an alternative method for high yield transfer is to mount the stencil onto a flexural stage that can correct for orientational misalignments 31 . The membrane transfer process is also extremely effective when used with flexible substrates 32 .…”

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confidence: 99%

High resolution fabrication of nanostructures using controlled proximity nanostencil lithography

Jain

Aernecke

Liberman

et al. 2014

Applied Physics Letters

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Nanostencil lithography has a number of distinct benefits that make it an attractive nanofabrication processes, but the inability to fabricate features with nanometer precision has significantly limited its utility. In this paper, we describe a nanostencil lithography process that provides sub-15 nm resolution even for 40-nm thick structures by using a sacrificial layer to control the proximity between the stencil and substrate, thereby enhancing the correspondence between nanostencil patterns and fabricated nanostructures. We anticipate that controlled proximity nanostencil lithography will provide an environmentally stable, clean, and positive-tone candidate for fabrication of nanostructures with high-resolution.Significant advances in device physics across optical, electrical, and magnetic modalities have been enabled by the ability to fabricate structures with nanoscale precision. There is currently a large variety of top-down nanostructure fabrication methods available, such as electron beam lithography 1 ,

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Integration of Solid-State Nanopores in Microfluidic Networks via Transfer Printing of Suspended Membranes

Cited by 42 publications

References 31 publications

Heterogeneous sub-continuum ionic transport in statistically isolated graphene nanopores

Heterogeneous sub-continuum ionic transport in statistically isolated graphene nanopores

Decoding long nanopore sequencing reads of natural DNA

High resolution fabrication of nanostructures using controlled proximity nanostencil lithography

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