Integration of Signals from Receptor Tyrosine Kinases and G Protein-Coupled Receptors

Lowes, Vicki L.; Ip, Nancy Y.; Wong, Yung Hou

doi:10.1159/000057317

Cited by 131 publications

(96 citation statements)

References 113 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Probably, Hoey is dealing with the so-called transactivation of growth factors tyrosine kinase (TK) receptors by PTHrP G-protein-coupled receptor (GPCR) (Linseman et al, 1995;Lowes et al, 2002). We observed a similar phenomenon in 2002 while culturing skin fibroblasts in the presence of serum (then of growth factors) .…”

Section: Sirmentioning

confidence: 68%

PTHrP on MCF-7 breast cancer cells: a growth factor or an antimitogenic peptide?

Maioli

Fortino

2004

Br J Cancer

View full text Add to dashboard Cite

Section: Sirmentioning

confidence: 68%

PTHrP on MCF-7 breast cancer cells: a growth factor or an antimitogenic peptide?

Maioli

Fortino

2004

Br J Cancer

View full text Add to dashboard Cite

“…Several studies have found that GPCRs transactivate RTKs that exert neurotrophic effects, such as the insulin growth factor (IGF)-1 receptor, EGFR, and TrkA/B [37][38][39] . PF has been studied for decades and has many effects in vivo and in vitro, several of which are independent of A 1 R [3,20] .…”

Section: Discussionmentioning

confidence: 99%

Paeoniflorin ameliorates ischemic neuronal damage in vitro via adenosine A1 receptor-mediated transactivation of epidermal growth factor receptor

Zhong

Song

et al. 2015

Acta Pharmacol Sin

View full text Add to dashboard Cite

Aim: Paeoniflorin from Chinese herb Paeoniae Radix has been shown to ameliorate middle cerebral artery occlusion-induced ischemia in rats. The aim of this study was to investigate the mechanisms underlying the neuroprotective action of PF in cultured rat cortical neurons. Methods: Primary cultured cortical neurons of rats were subjected to oxygen-glucose deprivation and reoxygenation (OGD/R) insult. Cell survival was determined using MTT assay. HEK293 cells stably transfected with A 1 R (HEK293/A 1 R) were used for detailed analysis. Phosphorylation of the signaling proteins was evaluated by Western blot or immunoprecipitation. Receptor interactions were identified using co-immunoprecipitation and immunofluorescence staining. Results: Paeoniflorin (10 nmol/L to 1 μmol/L) increased the survival of neurons subjected to OGD/R. Furthermore, paeoniflorin increased the phosphorylation of Akt and ERK1/2 in these neurons. These effects were blocked by PI3K inhibitor wortmannin or MEK inhibitor U0126. Paeoniflorin also increased the phosphorylation of Akt and ERK1/2 in HEK293/A 1 R cells. Both A 1 R antagonist DPCPX and EGFR inhibitor AG1478 not only blocked paeoniflorin-induced phosphorylation of ERK1/2 and Akt in HEK293/A 1 R cells, but also paeoniflorin-increased survival of neurons subjected to OGD/R. In addition, paeoniflorin increased the phosphorylation of Src kinase and activation of MMP-2 in HEK293/A 1 R cells. Both Src inhibitor PP2 and MMP-2/MMP-9 inhibitor BiPs not only blocked paeoniflorininduced phosphorylation of ERK1/2 (and Akt) in HEK293/A 1 R cells, but also paeoniflorin-increased survival of neurons subjected to OGD/R. Conclusion: Paeoniflorin promotes the survival of cultured cortical neurons by increasing Akt and ERK1/2 phosphorylation via A 1 Rmediated transactivation of EGFR.

show abstract

“…These data suggest that CDS is monitoring appropriate cellular physiological changes, as it is known that PTK and GPCR signaling pathways overlap and that similar cellular physiological outputs will manifest from stimulation of members of each of these families. [29][30][31][32][33] within a receptor family, different receptors of the same class tend to produce the same kinds of patterns, whereas receptors of different classes produce different patterns. However, patterns produced from receptors of 2 different families may be similar due to the convergent nature of the physiological responses elicited.…”

Section: Arrow) Hela Cell Patterns (A-d) Cho Cell Patterns (E F)mentioning

confidence: 99%

Cellular Dielectric Spectroscopy: A Powerful New Approach to Label-Free Cellular Analysis

Ciambrone¹,

Liu²,

Lin³

et al. 2004

SLAS Discovery

View full text Add to dashboard Cite

The past decade has seen a number of significant changes in identifying higher quality lead compounds earlier in the drug discovery process. Cell-based assay technologies yielding high-content information have emerged to achieve this goal. Although most of these systems are based on fluorescence detection, this article describes the development and application of an innovative cellular assay technology based on radio frequency spectrometry and bioimpedance measurements. Using this technique, the authors have discovered a link between cellular bioimpedance changes and receptor-mediated signal transduction events. By performing dielectric spectroscopy of cells across a spectrum of frequencies (1 KHz to 110 MHz), a series of receptorspecific, frequency-dependent impedance patterns is collected. These raw data patterns are used to determine the identity of the cellular receptor-signaling pathway being tested and to quantify stimulation endpoints and kinetics. The authors describe the application of this technology to the analysis of ligand-induced cellular responses mediated by the 3 major classes of Gprotein-coupled receptors (GPCRs) and protein tyrosine kinase receptors. This single assay platform can be used with ease to monitor G s , G i , and G q GPCRs without the need for chimeric or promiscuous G-proteins, fluorophors, or tagged proteins. In contrast to other methods of monitoring cellular signal transduction, this approach provides high information content in a sim-

show abstract

Integration of Signals from Receptor Tyrosine Kinases and G Protein-Coupled Receptors

Cited by 131 publications

References 113 publications

PTHrP on MCF-7 breast cancer cells: a growth factor or an antimitogenic peptide?

PTHrP on MCF-7 breast cancer cells: a growth factor or an antimitogenic peptide?

Paeoniflorin ameliorates ischemic neuronal damage in vitro via adenosine A1 receptor-mediated transactivation of epidermal growth factor receptor

Cellular Dielectric Spectroscopy: A Powerful New Approach to Label-Free Cellular Analysis

Contact Info

Product

Resources

About