Integration of pharmacodynamics, network pharmacology and metabolomics to elucidate effect and mechanism of Artemisia capillaris Thunb. in the treatment of jaundice

Yang, Qing; Wu, Xubo; Pan, Zhiyu; Guan, Ruifang; Yang, Ping; Liu, Ye; Yang, Xiaolei; Du, Wandi; Liang, Jingru; Hu, Jiarong; Cai, Weimin; Guo, Ma

doi:10.1016/j.jep.2022.115943

Cited by 6 publications

(4 citation statements)

References 43 publications

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“…Data were imported into SIMCA P14.1 and scaled using Pareto scaling for multivariate statistical analysis, including principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA). Scatter points in the OPLS-DA S-plot and variables with VIP > 1 were identified as potential biomarkers for further analysis [ 21 ]. T-tests were performed to determine if there were significant differences in the relative peak areas of biomarkers between groups, selecting metabolites with statistical significance ( P < 0.05).…”

Section: Methodsmentioning

confidence: 99%

Saiga antelope horn suppresses febrile seizures in rats by regulating neurotransmitters and the arachidonic acid pathway

Wu,

Song,

Zhao

et al. 2024

Chin Med

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Background Saiga antelope horn (SAH) is a traditional Chinese medicine for treating febrile seizure (FS) with precise efficacy, but its mechanism of action and functional substances are still unclear. Given the need for further research on SAH, our group conducted studies to elucidate its mechanisms and active substances. Methods An FS rat pup model was constructed through intraperitoneal injection of LPS and hyperthermia induction. Behavioural indicators of seizures, hippocampal histopathological alterations, serum levels of inflammatory cytokines and hippocampal levels of neurotransmitters were observed and measured to investigate the effects of SAH on FS model rats. Hippocampal metabolomics and network pharmacology analyses were conducted to reveal the differential metabolites, key peptides and pathways involved in the suppression of FS by SAH. Results SAH suppressed FS, decreased the inflammatory response and regulated the Glu-GABA balance. Metabolomic analysis revealed 13 biomarkers of FS, of which SAH improved the levels of 8 differential metabolites. Combined with network pharmacology, a “biomarker-core target-key peptide” network was constructed. The peptides of SAH, such as YGQL and LTGGF, could exert therapeutic effects via the arachidonic acid pathway. Molecular docking and ELISA results indicated that functional peptides of SAH could bind to PTGS2 target, inhibiting the generation of AA and its metabolites in hippocampal samples. Conclusion In summary, the functional peptides contained in SAH are the main material basis for the treatment of FS, potentially acting through neurotransmitter regulation and the arachidonic acid pathway. Graphical abstract

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Section: Methodsmentioning

confidence: 99%

Saiga antelope horn suppresses febrile seizures in rats by regulating neurotransmitters and the arachidonic acid pathway

Wu,

Song,

Zhao

et al. 2024

Chin Med

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“…Furthermore, AC is also a folk delicious wild vegetable eaten for thousands of years and is the key ingredient of the traditional snack “Qingming ba” in China. As one of the most commonly used herbs, AC has shown good results in the treatment of jaundice and liver diseases ( Yang et al, 2023 ). However, research on AC has mainly focused on its efficacy and biological activity in medicine, while research on its nutritional role as food, especially on its protein is still scarce and remains a mystery.…”

Section: Introductionmentioning

confidence: 99%

A promising Artemisia capillaris Thunb. Leaf proteins with high nutrition, applicable function and excellent antioxidant activity

Wei,

Wang,

Chen

et al. 2024

Food Chemistry: X

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“…Traditional network analysis is typically used to predict potential targets and molecular mechanisms of TCM in disease treatment, using data mining and scientific computing techniques based on public databases ( Yang et al, 2023 ). Network analysis can reveal the patterns and rules that govern the associations between drugs and diseases, enabling efficient screening of potential drug targets and pathways with therapeutic effects.…”

Section: Introductionmentioning

confidence: 99%

“…Integrated of network analysis and metabolomics has unique advantages in the study of TCM formulas, as it can reveal the intricate mechanisms of action within the body from multiple perspectives, including metabolites of botanical drug, targets, pathways, and metabolites in vivo ( Zhao Q. et al, 2022 ). Network analysis and metabolomics have successfully been applied to uncover the mechanisms of several TCM formulas in liver disease treatment ( Yu et al, 2019 ; Wang et al, 2022 ; Yang et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Integrated network analysis and metabolomics reveal the molecular mechanism of Yinchen Sini decoction in CCl4-induced acute liver injury

Zheng,

Shi,

Meng

et al. 2023

Front. Pharmacol.

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Objective: Yinchen Sini decoction (YCSND), a traditional Chinese medicine (TCM) formula, plays a crucial role in the treatment of liver disease. However, the bioactive constituents and pharmacological mechanisms of action remain unclear. The present study aimed to reveal the molecular mechanism of YCSND in the treatment of acute liver injury (ALI) using integrated network analysis and metabolomics.Methods: Ultra-high-performance liquid chromatography coupled with Q-Exactive focus mass spectrum (UHPLC-QE-MS) was utilized to identify metabolites in YCSND, and high-performance liquid chromatography (HPLC) was applied to evaluate the quality of four botanical drugs in YCSND. Cell damage and ALI models in mice were established using CCl4. 1H-NMR metabolomics approach, along with histopathological observation using hematoxylin and eosin (H&E), biochemical measurements, and reverse transcription quantitative real-time PCR (RT-qPCR), was applied to evaluate the effect of YCSND on CCl4- induced ALI. Network analysis was conducted to predict the potential targets of YCSND in ALI.Result: Our results showed that 89 metabolites in YCSND were identified using UHPLC-QE-MS. YCSND protected against ALI by reducing the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and malondialdehyde (MDA) contents and increasing those of superoxide dismutase (SOD), and glutathione (GSH) both in vivo and in vitro. The 1H-NMRmetabolic pattern revealed that YCSND reversed CCl4-induced metabolic abnormalities in the liver. Additionally, the Kyoto Encyclopedia of Genes and Genome (KEGG) pathway enrichment analysis identified five pathways related to liver injury, including the PI3K-AKT, MAPK, HIF-1, apoptosis, and TNF signaling pathways. Moreover, RT-qPCR showed YCSND regulated the inflammatory response (Tlr4, Il6, Tnfα, Nfκb1, Ptgs2, and Mmp9) and apoptosis (Bcl2, Caspase3, Bax, and Mapk3), and inhibited PI3K-AKT signaling pathway (Pi3k and Akt1). Combined network analysis and metabolomics showed a link between the key targets (Tlr4, Ptgs2, and Mmp9) and vital metabolites (choline, xanthine, lactate, and 3-hydroxybutyric acid) of YCSND in ALI.Conclusion: Overall, the results contribute to the understanding of the therapeutic effects of YCSND on ALI, and indicate that the integrated network analysis and metabolomics could be a powerful strategy to reveal the pharmacological effects of TCM.

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Integration of pharmacodynamics, network pharmacology and metabolomics to elucidate effect and mechanism of Artemisia capillaris Thunb. in the treatment of jaundice

Cited by 6 publications

References 43 publications

Saiga antelope horn suppresses febrile seizures in rats by regulating neurotransmitters and the arachidonic acid pathway

Saiga antelope horn suppresses febrile seizures in rats by regulating neurotransmitters and the arachidonic acid pathway

A promising Artemisia capillaris Thunb. Leaf proteins with high nutrition, applicable function and excellent antioxidant activity

Integrated network analysis and metabolomics reveal the molecular mechanism of Yinchen Sini decoction in CCl4-induced acute liver injury

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