Integration of Multiplex Bead Assays for Parasitic Diseases into a National, Population-Based Serosurvey of Women 15-39 Years of Age in Cambodia

Abstract

Collection of surveillance data is essential for monitoring and evaluation of public health programs. Integrated collection of household-based health data, now routinely carried out in many countries through demographic health surveys and multiple indicator surveys, provides critical measures of progress in health delivery. In contrast, biomarker surveys typically focus on single or related measures of malaria infection, HIV status, vaccination coverage, or immunity status for vaccine-preventable diseases (VPD). Here we describe an integrated biomarker survey based on use of a multiplex bead assay (MBA) to simultaneously measure antibody responses to multiple parasitic diseases of public health importance as part of a VPD serological survey in Cambodia. A nationally-representative cluster-based survey was used to collect serum samples from women of child-bearing age. Samples were tested by MBA for immunoglobulin G antibodies recognizing recombinant antigens from Plasmodium falciparum and P. vivax, Wuchereria bancrofti, Toxoplasma gondii, Taenia solium, and Strongyloides stercoralis. Serologic IgG antibody results were useful both for generating national prevalence estimates for the parasitic diseases of interest and for confirming the highly focal distributions of some of these infections. Integrated surveys offer an opportunity to systematically assess the status of multiple public health programs and measure progress toward Millennium Development Goals.

Tetanus Immunity among Women Aged 15 to 39 Years in Cambodia: a National Population-Based Serosurvey, 2012

“…Each assay well also included a bead coupled with Schistosoma japonicum glutathione-S-transferase as a negative-control protein and 18 additional beads coupled with antigen constructs from 12 parasitic or viral diseases. These results were published recently (42).…”

Tetanus Immunity Gaps in Children 5–14 Years and Men ≥ 15 Years of Age Revealed by Integrated Disease Serosurveillance in Kenya, Tanzania, and Mozambique

“…13,24,25 Including tetanus IgG testing by MBA in national serosurveys can help with monitoring progress towards and maintenance of MNTE. 13 WHO recommends integrating vaccinations given during health service contacts where possible, and opportunities to provide the WHO-recommended TTCV booster doses increasingly exist as countries introduce a second dose of measles-containing vaccine (MCV2) during the second year of life (2YL) and work to develop robust school-based programs for new vaccines that target preadolescents, such as human papillomavirus virus vaccine.…”

Multiplex serologic testing within a cross-sectional lymphatic filariasis sentinel site survey in coastal Kenya reveals community-level differences in IgG antibody responses to parasitic diseases and vaccines

“…A W. bancrofti Wb123-GST fusion protein was a kind gift from T. Nutman (NIH, Bethesda, MD). These proteins were coupled to SeroMap beads (Luminex Corp., Austin TX) using the protein quantities and buffer conditions previously described [23]. S. mansoni native soluble egg antigen (SEA) was a kind gift of E. Secor (CDC, Atlanta, GA), and recombinant S. mansoni Sm25 antigen was expressed using the Baculovirus system previously described [29].…”

“…A state-of-art multiplex bead assay (MBA) serological platform that enables simultaneous detection of antibodies against multiple antigens using a small volume of blood sample dried on filter paper [10 µL dried blood spots (DBS)] has been developed as a tool for integrated biomarker surveys [20][21][22]. The MBA has successfully been used to simultaneously measure antibody responses to multiple parasitic diseases of public health importance as part of a vaccine-preventable disease serological survey in Cambodia [23]. The platform has also been used to simultaneously assess IgG responses to a panel of malaria antigens [24,25].…”