Integration of Metabolome and Transcriptome Studies Reveals Flavonoids, Abscisic Acid, and Nitric Oxide Comodulating the Freezing Tolerance in Liriope spicata

Peng, Zhen; Wang, Ye; Zuo, Wen-Tian; Gao, Yuerong; Li, Runzhi; Yu, Chunxin; Liu, Ziyan; Zheng, Yi; Shen, Yuan‐Yue; Duan, Liu-Sheng

doi:10.3389/fpls.2021.764625

Cited by 24 publications

(15 citation statements)

References 41 publications

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“… Qin et al (2022) studied the response mechanism of two kinds of Lycium barbarum under salt stress through transcriptomics and metabolomics and revealed the intrinsic reason for the high salt tolerance of L. barbarum . By integrating metabolomic and transcriptomic studies, Peng et al (2021) revealed that flavonoids, abscisic acid, and nitric oxide jointly regulate the frost resistance of Liriope spicata and identified the involvement of the flavonoid pathway, abscisic acid biosynthesis, and the genes and metabolites of NO oxidative synthesis to construct a comprehensive network of frost resistance of L. spicata .…”

Section: Introductionmentioning

confidence: 99%

Mepiquat chloride inhibits soybean growth but improves drought resistance

et al. 2022

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Soybeans are an important economic crop. As the most widely used growth regulator globally, the molecular mechanism of mepiquat chloride (DPC) in soybean remains unknown. In this study, RNA sequencing technology combined with ultra-performance liquid chromatography and tandem mass spectrometry were used to analyze the changes in the leaf transcriptome and metabolomics of soybean leaves at the seedling stage under DPC stress. The results showed that differentially expressed genes related to photosynthesis and cell wall synthesis were significantly downregulated at the transcriptional level. In addition, the syntheses of gibberellin, zeatin, brassinolide, and other plant hormones were inhibited in the signal transduction pathway of plant hormones, thereby inhibiting plant growth. In contrast, at the metabolic level, the expression levels of flavonoid differential metabolites were significantly increased, and the proportions of flavonoids in the two varieties were 61.5 and 66%, respectively. The combined analysis of transcriptome and metabolomics showed that the differential expressed genes and metabolites were mainly enriched in the isoflavonoid biosynthesis and flavonoid biosynthesis pathways. Principally, DPC inhibited plant growth but improved drought resistance. Our study is the first to report the molecular mechanism of DPC regulation in soybean, providing useful insights into the rational application of DPC in soybean.

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Section: Introductionmentioning

confidence: 99%

Mepiquat chloride inhibits soybean growth but improves drought resistance

et al. 2022

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“…Secondary metabolites participate in plant growth, development, and adaptation, and may in addition play important roles in plant defense against pathogens and abiotic stress ( Bednarek and Osbourn, 2009 ; Peng et al., 2021 ). Triterpenoid saponins are known as a vital class of secondary metabolites with important medicinal value ( Ge et al., 2016 ; Yan et al., 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis reveals that jasmonic acid biosynthesis and signaling is associated with the biosynthesis of asperosaponin VI in Dipsacus asperoides

Zheng

Huang

et al. 2022

Front. Plant Sci.

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Dipsacus asperoides is a perennial herb, the roots of which are abundant in asperosaponin VI, which has important medicinal value. However, the molecular mechanism underlying the biosynthesis of asperosaponin VI in D. asperoides remains unclear. In present study, a comprehensive investigation of asperosaponin VI biosynthesis was conducted at the levels of metabolite and transcript during root development. The content of asperosaponin VI was significantly accumulated in two-leaf stage roots, and the spatial distribution of asperosaponin VI was localized in the xylem. The concentration of asperosaponin VI gradually increased in the root with the development process. Transcriptome analysis revealed 3916 unique differentially expressed genes (DEGs) including 146 transcription factors (TFs) during root development in D. asperoides. In addition, α-linolenic acid metabolism, jasmonic acid (JA) biosynthesis, JA signal transduction, sesquiterpenoid and triterpenoid biosynthesis, and terpenoid backbone biosynthesis were prominently enriched. Furthermore, the concentration of JA gradually increased, and genes involved in α-linolenic acid metabolism, JA biosynthesis, and triterpenoid biosynthesis were up-regulated during root development. Moreover, the concentration of asperosaponin VI was increased following methyl jasmonate (MeJA) treatment by activating the expression of genes in the triterpenoid biosynthesis pathway, including acetyl-CoA acetyltransferase (DaAACT), 3-hydroxy-3-methylglutaryl coenzyme A synthase (DaHMGCS), 3-hydroxy-3-methylglutaryl coenzyme-A reductase (DaHMGCR). We speculate that JA biosynthesis and signaling regulates the expression of triterpenoid biosynthetic genes and facilitate the biosynthesis of asperosaponin VI. The results suggest a regulatory network wherein triterpenoids, JA, and TFs co-modulate the biosynthesis of asperosaponin VI in D. asperoides.

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“…at low temperatures (24). Peng et al studied L. Spicata's avonoid biosynthesis, carbohydrate metabolism, amino acid metabolism, lipid metabolism, and signaling pathways are signi cantly enriched in response to cryostress (25). Lv et al found that the differential expression of P-coumarinamide, D-proline, betaine, and chlorogenic acid metabolites in wheat under low temperature conditions, and seven upregulated metabolites and eight upregulated key enzymes were also closely involved in the biosynthetic pathway of sucrose and amino acids (26).…”

Section: Introductionmentioning

confidence: 99%

Metabolomics-based Exploration the Response Mechanisms of Saussurea involucrata Leaves Under Different Levels of Cold Stress

Sun

Zhu

2023

Preprint

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Saussurea involucrata (Sik.) have high cold resistance and important medicinal value. However, the metabolomics under low temperature conditions is still not fully explained. Understanding the changes of metabolites under low temperature conditions is conducive to in-depth research and rational development and utilization. Methods: Ultra-performance liquid chromatography and tandem mass spectrometry were used to analyze the metabolites in the leaves of Sik. under four different low temperature stress conditions. Results: A total of 753 metabolites were identified, and 360 different metabolites were identified according to the Kyoto Encyclopedia of Genes and Genomes (KEGG) involved in the biosynthesis of secondary metabolites and amino acids and sugars. Sucrose and trehalose synthesis, glycolysis, tricarboxylic acid cycle, pentose phosphate pathway, glutamic acid-mediated proline biosynthesis, purine metabolism, amino acid metabolism, phenylpropane synthesis pathway metabolites all respond to low temperature stress. Under cold stress conditions, carbohydrates in Sik. leaves accumulate first than under freezing conditions, and the lower the temperature under freezing conditions, the less amino acids accumulate, while the phenolic substances increase. However, after cold domestication, purines and phenolic substances decrease under freezing conditions, and amino acids accumulate significantly. Conclusion: The metabolic network of Sik. leaves under different low temperature stress conditions was proposed, which provided a reference for further exploration of the metabolic network related to low temperature stress tolerance of Sik.

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Integration of Metabolome and Transcriptome Studies Reveals Flavonoids, Abscisic Acid, and Nitric Oxide Comodulating the Freezing Tolerance in Liriope spicata

Cited by 24 publications

References 41 publications

Mepiquat chloride inhibits soybean growth but improves drought resistance

Mepiquat chloride inhibits soybean growth but improves drought resistance

Transcriptome analysis reveals that jasmonic acid biosynthesis and signaling is associated with the biosynthesis of asperosaponin VI in Dipsacus asperoides

Metabolomics-based Exploration the Response Mechanisms of Saussurea involucrata Leaves Under Different Levels of Cold Stress

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