Integration of jasmonic acid and light irradiation for enhancement of anthocyanin biosynthesis in Vitis vinifera suspension cultures

Zhang, Wei; Curtin, Chris; Kikuchi, Mami; Franco, Christopher M. M.

doi:10.1016/s0168-9452(01)00586-6

Cited by 109 publications

(81 citation statements)

References 27 publications

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“…Recently, tissue culturing has become an important biotechnological technique to produce various secondary metabolites including anthocyanins on a mass scale for industrial and academic interest (Hirner et al 2001;Gantet and Memelink 2002). Despite the fact that some plant species have been shown to produce anthocyanins in cell cultures, little information of the cell culture techniques that are capable of producing in vitro anthocyanins has been reported in rose.The biosynthesis of anthocyanins in plant cell culture is stimulated by not only genetic factors but also environmental factors such as light, temperature, growth regulators and nutrition (Sato et al 1996;Zhang et al 1997;Zhang et al 2002). The identity of the environmental stimuli that lead to the accumulation of anthocyanins in rose is of considerable interest.…”

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confidence: 99%

Differential expression of anthocyanin biosynthesis genes in suspension culture cells of Rosa hybrida cv. Charleston

Hennayake

Takagi

Nishimura

et al. 2006

Plant Biotechnology

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A suspension cultured cell line was established from the cultivar of Rosa hybrida 'Charleston' as a study model to understand the response of the anthocyanin biosynthesis pathway to environmental cues. The major identified anthocyanin in cell cultures was cyanidin 3-glucoside (chrysanthemin). The anthocyanin yield was enhanced by culturing cells in the EM medium with added sucrose at high concentration under additional UV-B radiation to white light. Three cDNA fragments were cloned with degenerate primers by RT-PCR and the obtained sequences shared high homology with putative key enzymes (DFR, ANS, and UF3GT) of other species. The expression levels of these three genes were promoted under optimum conditions for anthocyanin accumulation. These results suggest that expression levels of these genes were closely correlated with a temporal buildup of anthocyanins in response to environmental factors.Key words: Anthocyanin, chrysanthemin, cyanin, Rosa hybrida, suspension cell culture.Plant Biotechnology 23, 379-385 (2006) Original PaperThis article can be found at http://www.jspcmb.jp/ pelargonidin, cyanidin and peonidin type anthocyanins (Biolley et al. 1994). Recently, tissue culturing has become an important biotechnological technique to produce various secondary metabolites including anthocyanins on a mass scale for industrial and academic interest (Hirner et al. 2001;Gantet and Memelink 2002). Despite the fact that some plant species have been shown to produce anthocyanins in cell cultures, little information of the cell culture techniques that are capable of producing in vitro anthocyanins has been reported in rose.The biosynthesis of anthocyanins in plant cell culture is stimulated by not only genetic factors but also environmental factors such as light, temperature, growth regulators and nutrition (Sato et al. 1996;Zhang et al. 1997;Zhang et al. 2002). The identity of the environmental stimuli that lead to the accumulation of anthocyanins in rose is of considerable interest. To date, Sato et al. (1996) has examined the intensity of white light that induces anthocyanin production in strawberry cell culture lines; however, no information was obtained about the effect of light quality on the production of anthocyanins in cultural cells. Therefore, the originality of this research is to investigate anthocyanin biosynthesis in cultural cells exposed to UV-B (290-320 nm) radiation.In this study, we used Rosa hybrida cultivar 'Charleston', a climbing type, originated in Australia. The open flowers of 'Charleston' undergo a striking color change from yellow to red over 10-12 days under natural daylight. We established a suspension cultured cell line from this cultivar of rose. Anthocyanin content was quantified by HPLC to determine the most efficient conditions, such as light including UV-B radiation and media, for continuous production of the pigments in the rose cell suspension. The expression levels of three genes encoding putative key enzymes (DFR, ANS, and UF3GT) for anthocyanin biosynthesis were ana...

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confidence: 99%

Differential expression of anthocyanin biosynthesis genes in suspension culture cells of Rosa hybrida cv. Charleston

Hennayake

Takagi

Nishimura

et al. 2006

Plant Biotechnology

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“…The reason may be the different feeding time did not influence the synthesis of quercetin. Zhang et al 33 reported a controversial opinion i.e., addition of jasmonic acid at different feeding time regulates the anthocyanin accumulation in cell suspension cultures of Vitis vinifera. Sajjalaguddam and Paladugu 34 noticed application of phenylalanine enhances quercetin content in in vitro cultures of Abutilon indicum.…”

Section: Effect Of Sucrose Concentration and Day Of Elicitation By Gamentioning

confidence: 99%

Isolation, Purification of Quercetin from in vitro Cell Suspension Culture of Caesalpinia pulcherrima and its Analysis by HPLC-DAD and NMR

Madanakumar¹,

Murukan²,

Lawarence³

et al. 2017

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Background: Caesalpinia pulcherrima, belongs to Caesapiniaceae, is a known medicinal plant widely distributed in India and is used in traditional medicine for the treatment of various ailments. Many phytochemicals are reported from the plant as potential source of crude drug. Materials and Methods: An efficient and simple reproducible protocol was developed for callus production using leaf explants of C. pulcherrima. The combination of 2, 4-D, kin and BA, was used for the callus induction. Subsequently, cell suspension culture and quercetin synthesis from in vitro callus was attempted. Role of effect of elicitors (Sucrose, ABA and salicylic acid) in cell suspension culture was carried in MS medium containing 2,4-D + BA + kinetin. Flavonoids was purified, fractionated by HPLC-DAD and NMR. Results: 2, 4-D (2.5 mg/L), BA (2.5 mg/L) + kin (1 mg/mL) was effective for maximum callus induction from leaf explants. Significant cell suspension culture was noticed with liquid MS medium containing 2,4-D (2 mg/L)+ BA (1mg/L)+ kinetin (1.5 mg/L). Sucrose, ABA and salicylic acid (SA) at different concentrations influenced cell biomass and quercetin accumulation. The addition of ABA/SA along with sucrose was found to have no remarkable effect on cell biomass and also quercetin synthesis. However, cells cultured in the medium fortified with 45 g/L sucrose without ABA/ SA showed the highest quercetin content (16.5 mg/g). Flavonoids was purified, fractionated by HPLC-DAD and NMR revealed the presence of 9 components such as quercetin, isoquercetin, quercetrin, rutin, quercetin 3-O-β-D-xyloside, quercetin 3-Oarabinopyranoside, quercetin 3-O-α-arabinopyranosyl (1→2) β-galactopyranoside, isorhamnetin 3-O-rutinoside and an unknown compound. Conclusion: C. pulcherima reveals significant synthesis of quercetin. Quercetin content recorded in cell suspension culture was significantly higher compared with in vivo plants grown in fields and the compounds were identified by NMR.

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“…The factors affecting the level of anthocyanins include: culture filtrates and cell extracts of fungal and bacterial origin, UV light, fluridone, jasmonates, ethylene, β-glucan, chitosan and inorganic ions (Zhang et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…(Franceschi and Grimes, 1991), Arabidopsis thaliana (L.) Heynh. seedlings (Feys et al, 1994), in stems and leaves of the tulip (Tulipa gesneriana L.) (Saniewski et al, 1998), in a suspension culture of Vitis vinifera cells (Zhang et al, 2002;Curtin et al, 2003) and in cell cultures of Vaccinium pahalae (Fang et al, 1999). JA-Me added to growth medium induced anthocyanin accumulation and influenced on the composition in the sweet potato cell suspension cultures (Plata et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Elicitation of Anthocyanin Production in Roots of Kalanchoe blossfeldiana by Methyl Jasmonate

Góraj-Koniarska

Stochmal

Oleszek

et al. 2015

Acta Biologica Cracoviensia S. Botanica

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The influence of methyl jasmonate on anthocyanin accumulation in roots of Kalanchoe blossfediana plants was studied. Methyl jasmonate (JA-Me), at a concentration of 5.0 to 40.0 mg.l -1 , substantially increased anthocyanin accumulation in roots of intact plants, when it was applied as a solution under natural light conditions. The production of anthocyanin depended on the concentration of methyl jasmonate and the age of the plant. The stimulatory effect was higher in older plants of K. blossfeldiana than in younger ones. When leaves were removed methyl jasmonate slightly stimulated anthocyanin accumulation compared with intact plants. The obtained results indicate that leaves are necessary for the anthocyanin accumulation in the roots. In isolated roots methyl jasmonate did not affect the accumulation of anthocyanins in light conditions. Seven anthocyanins were documented in the roots of control plants and 8 anthocyanins in the roots of JA-Me treated ones. JA-Me increased the level of anthocyanins in roots of old K. blossfeldiana plants 6.8, 6.0 and 3.6-folds, after 4, 8 and 14-days of treatment, respectively.

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Integration of jasmonic acid and light irradiation for enhancement of anthocyanin biosynthesis in Vitis vinifera suspension cultures

Cited by 109 publications

References 27 publications

Differential expression of anthocyanin biosynthesis genes in suspension culture cells of Rosa hybrida cv. Charleston

Differential expression of anthocyanin biosynthesis genes in suspension culture cells of Rosa hybrida cv. Charleston

Isolation, Purification of Quercetin from in vitro Cell Suspension Culture of Caesalpinia pulcherrima and its Analysis by HPLC-DAD and NMR

Elicitation of Anthocyanin Production in Roots of Kalanchoe blossfeldiana by Methyl Jasmonate

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