Integration of isothermal amplification methods in microfluidic devices: Recent advances

Giuffrida, Maria Chiara; Spoto, Giuseppe

doi:10.1016/j.bios.2016.11.045

Cited by 136 publications

(75 citation statements)

References 94 publications

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“…3. Readers are referred to recent reviews for a more comprehensive overview of isothermal nucleic acid amplification techniques (Bi et al, 2017;Deng et al, 2017;Giuffrida and Spoto, 2017;Zhao et al, 2015). (A) An aptamer-based fluorescence assay for the detection of S. Typhimurium using aptamers either modified with a fluorescent label (FAM) or linked to magnetic nanoparticles (Duan et al, 2013).…”

Section: Aptamer-based Pathogen Detection Based On the Isothermal Nucmentioning

confidence: 99%

Nucleic acid aptamer-based methods for diagnosis of infections

Park

2018

Biosensors and Bioelectronics

135

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Infectious diseases are a serious global problem, which not only take an enormous human toll but also incur tremendous economic losses. In combating infectious diseases, rapid and accurate diagnostic tests are required for pathogen identification at the point of care (POC). In this review, investigations of diagnostic strategies for infectious diseases that are based on aptamers, especially nucleic acid aptamers, oligonucleotides that have high affinities and specificities toward their targets, are described. Owing to their unique features including low cost of production, easy chemical modification, high chemical stability, reproducibility, and low levels of immunogenicity and toxicity, aptamers have been widely utilized as bio-recognition elements (bio-receptors) for the development of infection diagnostic systems. We discuss nucleic acid aptamer-based methods that have been developed for diagnosis of infections using a format that organizes discussion according to the target pathogenic analytes including toxins or proteins, whole cells and nucleic acids. Also included is, a summary of recent advances made in the sensitive detection of pathogenic bacteria utilizing the isothermal nucleic acid amplification method. Lastly, a nucleic acid aptamer-based POC system is described and future directions of studies in this area are discussed.

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Section: Aptamer-based Pathogen Detection Based On the Isothermal Nucmentioning

confidence: 99%

Nucleic acid aptamer-based methods for diagnosis of infections

Park

2018

Biosensors and Bioelectronics

135

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“…LAMP (12), RPA (14), NASBA (15) and CPA (16)) have been successfully tested for amplification of single 15 nucleic acid molecule, HOLMESv2 therefore has great potential for further clinical diagnostics at the single-molecule level. In addition, HOLMESv2 can also be integrated into present diagnostic instruments and platforms, including the portable lateral flow assay devices for convenient detection (17), the microfluidic platform for multiple targets (18,19), the quantitative PCR machine for relative quantification and the digital PCR system for absolute quantification (20). Supplementary Materials: 10…”

Section: Main Textmentioning

confidence: 99%

CRISPR-Cas12b-assisted nucleic acid detection platform

Li²,

Wang

2018

Preprint

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Rapid molecular diagnostic technology is very useful in many areas, including public health, environmental testing and criminal investigation. We recently showed that Cas12a had trans-cleavage activity upon collateral single-stranded DNA (ssDNA), with which the HOLMES platform (one-HOur Low-cost Multipurpose highly Efficient System) was developed. Here, we combine the thermophilic Cas12b, which also has the ssDNA trans-cleavage activity, with Loop-5 Mediated Isothermal Amplification (LAMP), and create HOLMESv2. In HOLMESv2, LAMP amplification and Cas12b trans-cleavage can be integrated into a one-step system with a constant temperature, which therefore brings much convenience in nucleic acid detection. Moreover, we also simplify the RNA detection procedures in HOLMESv2, using an RNA-dependent DNA polymerase for amplification and therefore omitting an extra reverse transcription step. 10 One Sentence Summary: We combine LAMP and Cas12b to develop HOLMESv2 for conveniently detecting target nucleic acid in a one-step approach.

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“…Отдельным направлением является разработка различных модификаций микрочипов, что позволит сделать диагностику с помощью LAMP еще более доступной [58][59][60]. Таким образом, LAMP оказалась весьма перспективным методом, уже входящим в широкое применение.…”

Section: визуализация продуктов реакцииunclassified

Loop-mediated isothermal amplification: an effective method for express-diagnostics of cancer

Макарова¹,

Зотиков²,

Белякова³

et al. 2018

Cancer Urology

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The review is devoted to loop-mediated isothermal amplification (LAMP) – a novel molecular diagnostic method that has recently become increasingly popular. Unlike polymerase chain reaction, LAMP does not require thermal cycling; DNA or RNA amplification occurs at a constant temperature (about 65 °C) with 4 or 6 primers. This is a fast, highly-sensitive, and highly specific method, which does not require expensive equipment, where visual detection of the reaction products is performed by the unaided eye. LAMP is successfully used for the diagnosis of multiple viruses, bacteria, and other pathogens (including those in food). Moreover, it can be applied for the detection of singlenucleotide polymorphisms. Recently, a modified LAMP assay – one-step nucleic acid amplification (OSNA) – was validated for metastasis detection. OSNA was demonstrated to have almost the same sensitivity and specificity as standard diagnostic methods (sometimes even higher). Particular attention is paid to the mechanism of LAMP, primer design, and diagnostics of cancer using OSNA.

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Integration of isothermal amplification methods in microfluidic devices: Recent advances

Cited by 136 publications

References 94 publications

Nucleic acid aptamer-based methods for diagnosis of infections

Nucleic acid aptamer-based methods for diagnosis of infections

CRISPR-Cas12b-assisted nucleic acid detection platform

Loop-mediated isothermal amplification: an effective method for express-diagnostics of cancer

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