Integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for isolation and identification of compounds in Psoralea corylifolia

Chen, Xueguo; Kong, Liang; Su, Xingye; Pan, Chensong; Ye, Mingliang; Zou, Hanfa

doi:10.1016/j.chroma.2005.06.067

Cited by 47 publications

(32 citation statements)

References 27 publications

(22 reference statements)

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“…Zou et al [67 -72] have performed much work on the separation of TCMs. They have constructed different effective comprehensive two-dimensional liquid chromatography modes and successfully applied them to the separation and identification of compounds in Rhizoma chuanxiong [67], G. biloba [68], honeysuckle [69], and Psoralea corylifolia [71]. Recently, 2-D LC, coupling a silicabonded HSA column to a silica monolithic octadecylsilica column, was developed by Zou et al, for analysis of the extract of Rheum palmatum L. [70].…”

Section: Multidimensional Separation Modes For Traditional Chinese Mementioning

confidence: 99%

Recent developments and contributions from Chinese scientists in multidimensional separations for proteomics and traditional Chinese medicines

Gao

Deng

Lin

et al. 2007

J of Separation Science

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ReviewRecent developments and contributions from Chinese scientists in multidimensional separations for proteomics and traditional Chinese medicinesThe most basic task in proteomics remains the detection and identification of proteins from a biological sample, and the most traditional way to achieve this goal consists in protein separations performed by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). Yet the 2-D PAGE-mass spectrometry (MS) approach has its drawbacks with regard to automation, sensitivity, and throughput. Consequently, considerable effort has been devoted to the development of non-gel-based proteome separation technologies in an effort to alleviate the shortcomings of 2-D PAGE. In addition, traditional Chinese medicines (TCMs), due to their long period of clinical testing and reliable therapeutic efficacy, are attracting increased global attention. However, hundreds or even thousands of components are usually present in TCMs, which results in great difficulties of separation. As a mainstream separation tool, multidimensional liquid separation systems have shown powerful separation ability, high peak capacity, and excellent detectability in the analysis of complex samples including biological samples and TCMs, etc. Therefore, this review emphasizes the most recent advances in multidimensional liquid chromatography and capillary electrophoresis-based separation techniques, and the corresponding applications in proteomics and TCMs. In view of the significant contributions from Chinese scientists, this review focuses mainly on the work of Chinese scientists in the above fields.

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Section: Multidimensional Separation Modes For Traditional Chinese Mementioning

confidence: 99%

Recent developments and contributions from Chinese scientists in multidimensional separations for proteomics and traditional Chinese medicines

Gao

Deng

Lin

et al. 2007

J of Separation Science

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show abstract

“…However, the rapid and reliable identification of chemical components contained in natural plants remains still a great challenge, despite recent advances in various analytical technologies. Although the previously reported methodologies could successfully identify up to dozens of components from natural plants products (Wang et al 2004;Chen et al 2005;Zhu et al 2007), most of them have been limited to target components and depended largely on the use of reference compounds and/or the comparisons with the literature data. Considering that the reference compounds are always difficult to obtain and most components contained in natural plants are unknown, the previously reported methods are apparently insufficient for the global detection and identification of the complicated components in natural plants products.…”

Section: Introductionmentioning

confidence: 99%

Rapid Identification and Detection of Flavonoids Compounds From Bamboo Leaves by Lc-(Esi)-It-Tof/MS

Lv¹,

Dong²,

Zhang³

2012

BioResources

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This paper provides an applicable approach to identifying flavonoid compounds from bamboo leaves extracts, based on the use of the powerful Liquid Chromatography Electrospray Ionization source in combination with hybrid Ion Trap and high-resolution Time-of-flight Mass Spectrometry (LC-(ESI)-IT-TOF/MS). The strategy involves four procedural steps including searching flavonoid components based on an ultraviolet spectrum scan, getting the accurate mass of flavonoid components parent ion, retrieving the corresponding formula by software, and speculating as to the chemical structure according to mass spectrum decomposition rules. The presently developed methodology has been well proven to be useful and valuable by successful application to the identification of flavonoid components from Dendrocalamopsis oldham leaves. All of the 13 flavonoid components detected have been successfully identified by this approach, except that it failed to confirm 3 flavonoid component chemical structures. The calibration curves of two flavonoid components (orientin and vitexin) that had been identified in bamboo leaves showed a good linear fit (R 2 ≥ 0.9998) in the concentration range of 6.25 to 200 mg/L. The limits of detection (LOD) were less 0.02 mg/L (S/N=3), and the estimated limits of quantification (LOQ) were less 0.06 mg/L (S/N=10). Intra-and inter-day relative standard deviations were less than 1.04 and 1.82%, respectively.

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“…2D-LC combines two independent columns with different separation mechanisms in tandem mode. Depending on whether components can be eluted from the first dimension into the second dimension directly or not, 2D-LC methods can be classified as on-line [1][2][3] or off-line [4][5][6], respectively. Furthermore, depending on whether first dimensional components are eluted into the second dimension completely or not, 2D-LC methods can be classified as comprehensive [3,7,8] or heart cutting [9][10][11], respectively.…”

Section: Introductionmentioning

confidence: 99%

The development of an evaluation method for capture columns used in two-dimensional liquid chromatography

Cao

Wang

et al. 2011

Analytica Chimica Acta

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Cited by 47 publications

References 27 publications

Recent developments and contributions from Chinese scientists in multidimensional separations for proteomics and traditional Chinese medicines

Recent developments and contributions from Chinese scientists in multidimensional separations for proteomics and traditional Chinese medicines

Rapid Identification and Detection of Flavonoids Compounds From Bamboo Leaves by Lc-(Esi)-It-Tof/MS

The development of an evaluation method for capture columns used in two-dimensional liquid chromatography

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