Integration of Horse Manure Vermicompost Doses and Arbuscular Mycorrhizal Fungi to Improve Fruit Quality, and Soil Fertility in Tomato Field Facing Drought Stress

Abstract

Climate change poses major challenges for agriculture in arid and semi-arid regions, with drought conditions severely affecting water-intensive crops such as tomatoes. This study evaluates the efficacy of organic amendments, derived from horse manure, and arbuscular mycorrhizal fungi (AMF) on enhancing tomato (Solanum lycopersicum L.) fruit quality and soil health under semi-arid field conditions. The experimental design included two irrigation regimes (well-watered and drought stress) and two levels of vermicompost application (C1 5 t ha−1 and C2 10 t ha−1), applied individually or in combination with AMF. The results indicate that drought stress reduced tomato fruit growth and yield, while osmoprotectant accumulation, antioxidant enzyme activity, and bioactive compound levels increased, and the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity of tomato fruit also increased. Notably, the biostimulants application, especially (C1+AMF), counteracted the adverse effects of drought, compared to the control, by significantly enhancing fruit yields (60%), as well as increasing ascorbic acid levels (59%) and free amino acids content (90%). These treatments also improved the activity of bioactive compounds and nutrient uptake in the fruit. Furthermore, biostimulant application positively affected the physicochemical properties of soil. The results obtained confirm that the application of biostimulants can be suitable for improving crop sustainability and adaptability under conditions of water stress in semi-arid field regions.