Integration of five groups of POPs into one multi-analyte method for human blood serum analysis: An innovative approach within biomonitoring studies

Švarcová, Andrea; Lanková, Darina; Gramblicka, Tomas; Stupák, Michal; Hajšlová, Jana; Pulkrabová, Jana

doi:10.1016/j.scitotenv.2019.02.336

Cited by 22 publications

(9 citation statements)

References 36 publications

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“…Several methods have been developed for the quantitation of different chemical classes of PFASs in human serum and plasma, by implementing liquid chromatography coupled with tandem mass spectrometry as a suitable analytical technique for high sensitivity and selectivity. Before injection, plasma and serum samples are usually treated to remove proteins [41][42][43][44][45] and/or purified with solid-phase extraction (SPE) [16,[46][47][48][49][50][51][52][53][54][55][56][57][58]. However, only a few methods considered the emerging compounds HFPO-DA [16,43,44], F-53B 1 3 [16,53,56], DONA [16,43], or PFECHS [43,45].…”

Section: Introductionmentioning

confidence: 99%

Development and validation of an LC–MS/MS method for the quantitation of 30 legacy and emerging per- and polyfluoroalkyl substances (PFASs) in human plasma, including HFPO-DA, DONA, and cC6O4

et al. 2021

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Per-and polyfluoroalkyl substances (PFASs) include persistent organic pollutants whose spread is still ubiquitous. Efforts to substitute substances of high concern with fluorinated alternatives, such as HFPO-DA (GenX), DONA (ADONA), and cC6O4, have been made. The aim of this work was to develop and validate an isotopic dilution liquid chromatographytandem mass spectrometry (LC-MS/MS) method suitable to quantify 30 PFASs in human plasma. Analytes included legacy PFASs (PFOA, PFOS, and PFHxS), fluorinated alternatives (PFBA, PFBS, 6:2 FTSA, HFPO-DA, DONA, and cC6O4), and newly identified compounds (F-53B and PFECHS). The sample preparation was rapid and consisted of simple protein precipitation and centrifugation. Calibration standards and quality control solutions were prepared with a human pooled plasma containing relatively low background levels of the considered analytes. A complete validation was carried out: the lower limits of quantitation (LLOQs) ranged from 0.009 to 0.245 µg/L; suitable linearity (determination coefficients, R 2 0.989-0.999), precision (2.0-19.5%, relative standard deviation), and accuracy (87.9-113.1% of theoretical) were obtained for considered concentration ranges. No significant variations of analyte responses were recorded under investigated storage conditions and during matrix effect tests. The external verification confirmed the accuracy of the method, although limited to 12 analytes. The method was also applied to 38 human plasma samples to confirm its applicability. The developed assay is suitable for large-scale analyses of a wide range of legacy and emerging PFASs in human plasma. To our knowledge, this is the first published method including cC6O4 for human biomonitoring.

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Section: Introductionmentioning

confidence: 99%

Development and validation of an LC–MS/MS method for the quantitation of 30 legacy and emerging per- and polyfluoroalkyl substances (PFASs) in human plasma, including HFPO-DA, DONA, and cC6O4

et al. 2021

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“…Another method for the simultaneous extraction of flame retardants, perfluoroalkylated substances, PCBs, and OCPs was developed on the basis of a multistep solvent extraction and column cleanup. 18 Additionally, analytical methods used for identification and quantification have included gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry. This method, however, requires at least 3 mL of serum, and as such, there is no currently published analytical procedure for the simultaneous determination of PAEs, PAHs, PCBs, PBDEs, and pesticides (such as OCPs, OPPs, etc.)…”

Section: ■ Introductionmentioning

confidence: 99%

“…However, this method utilized four different sorbents as well as a genotoxic solvent, methyl tert -butyl ether, for extracting target analytes and removing crude lipids or co-extracts. Another method for the simultaneous extraction of flame retardants, perfluoroalkylated substances, PCBs, and OCPs was developed on the basis of a multistep solvent extraction and column cleanup . Additionally, analytical methods used for identification and quantification have included gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry.…”

Section: Introductionmentioning

confidence: 99%

Integration of 103 Semivolatile Organic Compounds into One Multianalyte Method for Human Serum Analysis: An Innovative Approach within Exposure Assessment

Wang

Huang

Zeng

et al. 2021

Environ. Sci. Technol. Lett.

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Semivolatile organic compounds (SVOCs) are frequently detected in the environment, and humans are exposed to a mixture of chemicals via multiple exposure pathways. However, there are few data on health outcomes caused by the cumulative exposure or toxicological interactions, due to the absence of simultaneous analytical methods. Herein, we present a method for simultaneous determination of 103 SVOCs, including seven phthalate esters, seven polychlorinated biphenyls, 16 polycyclic aromatic hydrocarbons, five polybromodiphenyl ethers, and 68 pesticides, in 0.5 mL of serum. The two-step extraction coupled with gas chromatography-tandem mass spectrometry was described with a single chromatographic run within 36.65 min. Methodological parameters such as recovery rates, matrix effects, and limits of detection were studied and complied with the validation guidelines. The absolute and relative recoveries ranged from 65.45% to 123.83%, and the relative standard deviations were ≤21.43%. The detection limits were <0.41 μg L–1. This method was then successfully applied to the analysis of 99 real serum samples. The developed protocol achieved a limit of quantification at a level of parts per billion for all compounds and presented benefits for the control of confounding factors, cumulative exposure, and risk assessment in population studies.

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“…Recently, this group has been supplemented with perfluoroalkylated compounds, in particular, perfluoroalkyl sulfonates and perfluorooctanoic acid. Svarcova et al [9] performed the multianalyte analysis of biological samples for POPs based on an innovative approach to the simultaneous determination of 78 halogen-containing organic POPs in human blood serum, namely, 40 flame retardants (including 7 new brominated and chlorinated flame retardants), 19 perfluoroalkanes, 11 organochlorine pesticides, and 8 polychlorinated biphenyls. The following two fractions of analytes were extracted from blood serum in the course of sample preparation for analysis: (I) a nonpolar hydrophobic fraction and (II) a more polar and hydrophilic one.…”

Section: Reviews Persistent Organic Pollutantsmentioning

confidence: 99%

Scopes of Bioanalytical Chromatography–Mass Spectrometry

Savelieva

2021

J Anal Chem

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The areas of application of modern bioanalytical chromatography-mass spectrometry are so extensive that any attempt to systematize them becomes subjective. It would be more correct to say that there is no such area of biology and medicine where chromatography-mass spectrometry would not find application. This article focuses on the areas of application of this technique that are either relatively new or insufficiently covered in recent reviews. State-of-the-art bioanalytical techniques have become multitargeted in terms of analytes and standardized in terms of matrices. The ability to detect trace concentrations of analytes in the presence of a huge number of biomatrix macrocomponents using chromatography-mass spectrometry is especially important for bioanalytical chemistry. In the target-oriented determination of persistent organic pollutants by chromatography-mass spectrometry, the main problem is the expansion of the list of analytes, including isomers. In the detection of exposures to unstable toxicants, the fragmented adducts of xenobiotics with biomolecules become target biomarkers along with hydrolytic metabolites. The exposome reflects the general exposure of a human being to total xenobiotics and the metabolic status reflects the physiological state of the body. Chromatography-mass spectrometry is a key technique in metabolomics. Metabolomics is currently used to solve the problems of clinical diagnostics and anti-doping control. Biological sample preparation procedures for instrumental analysis are being simplified and developed toward increasing versatility. Proteomic technologies with the use of various versions of mass spectrometry have found application in the development of new methods for diagnosing coronavirus infections.

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Integration of five groups of POPs into one multi-analyte method for human blood serum analysis: An innovative approach within biomonitoring studies

Cited by 22 publications

References 36 publications

Development and validation of an LC–MS/MS method for the quantitation of 30 legacy and emerging per- and polyfluoroalkyl substances (PFASs) in human plasma, including HFPO-DA, DONA, and cC6O4

Development and validation of an LC–MS/MS method for the quantitation of 30 legacy and emerging per- and polyfluoroalkyl substances (PFASs) in human plasma, including HFPO-DA, DONA, and cC6O4

Integration of 103 Semivolatile Organic Compounds into One Multianalyte Method for Human Serum Analysis: An Innovative Approach within Exposure Assessment

Scopes of Bioanalytical Chromatography–Mass Spectrometry

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