Integration of Ethylene and Auxin Signaling and the Developmental Consequences of Their Crosstalk

Muday, Gloria K.; Maloney, Gregory S.; Lewis, Daniel R.

doi:10.1007/978-94-017-9484-8_10

Cited by 4 publications

(3 citation statements)

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“…An effect of auxin on the regulation of endogenous ethylene biosynthesis has been reported in plant growth and development. Auxin and ethylene have overlapping functions in fruit ripening [ 22 ]. A high concentration of IAA in peach ( Prunus persica L.) fruit is necessary for ethylene biosynthesis during fruit ripening [ 23 , 24 ].…”

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confidence: 99%

Exogenous auxin-induced ENHANCER OF SHOOT REGENERATION 2 (ESR2) enhances femaleness of cucumber by activating theCsACS2gene

Niu

Zhao

et al. 2022

Horticulture Research

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Cucumber (Cucumis sativus L.) is a model for the study of sex differentiation in the last two decades. In cucumber, sex differentiation is mainly controlled by genetic material, but plant growth regulators can also influence or even change it. However, the effect of exogenous auxin application on cucumber sex differentiation is mostly limited in physiological level. In this study, we explored the effects of different exogenous auxin concentrations on the varieties with different mutant sex-controlling genotypes and found that there was a dosage effect of exogenous indole-3-acetic acid (IAA) on the enhancement of cucumber femaleness. Several ACC synthetase (ACS) family members could directly respond to the induction of exogenous IAA to improve endogenous ethylene synthesis, and this process can be independent on the previously identified sex-related ACC oxidase CsACO2. We further demonstrated that ENHANCER OF SHOOT REGENERATION 2 (ESR2), responding to the induction of exogenous auxin, could directly activate CsACS2 expression by combining the ERE cis-acting element regions in the promoter, and then increase endogenous ethylene content, which may induce femaleness. These findings reveal that exogenous auxin improves cucumber femaleness via inducing sex-controlling gene and promoting ethylene synthesis.

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Section: Introductionmentioning

confidence: 99%

Exogenous auxin-induced ENHANCER OF SHOOT REGENERATION 2 (ESR2) enhances femaleness of cucumber by activating theCsACS2gene

Niu

Zhao

et al. 2022

Horticulture Research

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“…The phytohormones, auxin and ethylene, regulate physiological and developmental processes in plants, synergistically controlling some responses, while antagonistically regulating others 1,2 . The morphological changes effected in roots by these two hormones are well characterized, with both hormones inhibiting primary root elongation and stimulating root hair formation.…”

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confidence: 99%

“…The morphological changes effected in roots by these two hormones are well characterized, with both hormones inhibiting primary root elongation and stimulating root hair formation. In contrast, the hormones have opposite effects on lateral root development, with auxin stimulating and ethylene inhibiting this process 1,3,4 .…”

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confidence: 99%

The dynamic response of the Arabidopsis root metabolome to auxin and ethylene is not predicted by changes in the transcriptome

Hildreth

Foley

Muday

et al. 2020

Sci Rep

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While the effects of phytohormones on plant gene expression have been well characterized, comparatively little is known about how hormones influence metabolite profiles. This study examined the effects of elevated auxin and ethylene on the metabolome of Arabidopsis roots using a highresolution 24 h time course, conducted in parallel to time-matched transcriptomic analyses. Mass spectrometry using orthogonal UPLC separation strategies (reversed phase and HILIC) in both positive and negative ionization modes was used to maximize identification of metabolites with altered levels. The findings show that the root metabolome responds rapidly to hormone stimulus and that compounds belonging to the same class of metabolites exhibit similar changes. The responses were dominated by changes in phenylpropanoid, glucosinolate, and fatty acid metabolism, although the nature and timing of the response was unique for each hormone. These alterations in the metabolome were not directly predicted by the corresponding transcriptome data, suggesting that post-transcriptional events such as changes in enzyme activity and/or transport processes drove the observed changes in the metabolome. These findings underscore the need to better understand the biochemical mechanisms underlying the temporal reconfiguration of plant metabolism, especially in relation to the hormone-metabolome interface and its subsequent physiological and morphological effects.are available in the supplementary materials (Table S1). Data mining for the specific metabolites rutin, IAA and ACC was performed by searching the detected masses of the features in the XCMS positive datasets for masses corresponding to [M + H] + ions and the negative XCMS datasets for masses corresponding to [M − H] − ions. Features were not observed that corresponded to either IAA or ACC, while the details regarding the assignment of rutin are included in Table S1. transcriptome data analysis. Microarray datasets for root samples generated under identical conditions 11,12 were examined for changes in the expression of genes associated with the synthesis, inactivation, or transport of hormone-responsive metabolites, identified from the KEGG database 78 or the recent literature (e.g., in the case of members of the GH, DAO, and PIN families). Results for genes exhibiting a SLR of ≥ 0.5 or ≤ −0.5 in all three biological replicates for at least one time point are presented in Tables 4 and 5, with the data for all examined genes provided in Datafile S5.

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Transcriptional sequencing and analysis of major genes involved in the adventitious root formation of mango cotyledon segments

Zhang

et al. 2017

Planta

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A total of 74,745 unigenes were generated and 1975 DEGs were identified. Candidate genes that may be involved in the adventitious root formation of mango cotyledon segment were revealed. Adventitious root formation is a crucial step in plant vegetative propagation, but the molecular mechanism of adventitious root formation remains unclear. Adventitious roots formed only at the proximal cut surface (PCS) of mango cotyledon segments, whereas no roots were formed on the opposite, distal cut surface (DCS). To identify the transcript abundance changes linked to adventitious root development, RNA was isolated from PCS and DCS at 0, 4 and 7 days after culture, respectively. Illumina sequencing of libraries generated from these samples yielded 62.36 Gb high-quality reads that were assembled into 74,745 unigenes with an average sequence length of 807 base pairs, and 33,252 of the assembled unigenes at least had homologs in one of the public databases. Comparative analysis of these transcriptome databases revealed that between the different time points at PCS there were 1966 differentially expressed genes (DEGs), while there were only 51 DEGs for the PCS vs. DCS when time-matched samples were compared. Of these DEGs, 1636 were assigned to gene ontology (GO) classes, the majority of that was involved in cellular processes, metabolic processes and single-organism processes. Candidate genes that may be involved in the adventitious root formation of mango cotyledon segment are predicted to encode polar auxin transport carriers, auxin-regulated proteins, cell wall remodeling enzymes and ethylene-related proteins. In order to validate RNA-sequencing results, we further analyzed the expression profiles of 20 genes by quantitative real-time PCR. This study expands the transcriptome information for Mangifera indica and identifies candidate genes involved in adventitious root formation in cotyledon segments of mango.

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Integration of Ethylene and Auxin Signaling and the Developmental Consequences of Their Crosstalk

Cited by 4 publications

References 155 publications

Exogenous auxin-induced ENHANCER OF SHOOT REGENERATION 2 (ESR2) enhances femaleness of cucumber by activating theCsACS2gene

Exogenous auxin-induced ENHANCER OF SHOOT REGENERATION 2 (ESR2) enhances femaleness of cucumber by activating theCsACS2gene

The dynamic response of the Arabidopsis root metabolome to auxin and ethylene is not predicted by changes in the transcriptome

Transcriptional sequencing and analysis of major genes involved in the adventitious root formation of mango cotyledon segments

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