Integration of epithelial patterning and morphogenesis inDrosophila ovarian follicle cells

Dobens, Leonard L.; Raftery, Laurel A.

doi:10.1002/(sici)1097-0177(200005)218:1<80::aid-dvdy7>3.0.co;2-8

Cited by 162 publications

(15 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The process of proliferation and differentiation of the follicle cells is complex and under stringent control [37-39]. One critical event is the cessation of mitosis in mid-oogenesis.…”

Section: Resultsmentioning

confidence: 99%

Notch signaling during development requires the function of awd, the Drosophila homolog of human metastasis suppressor gene Nm23

et al. 2014

View full text Add to dashboard Cite

BackgroundThe Drosophila abnormal wing discs (awd) belongs to a highly conserved family of genes implicated in metastasis suppression, metabolic homeostasis and epithelial morphogenesis. The cellular function of the mammalian members of this family, the Nm23 proteins, has not yet been clearly defined. Previous awd genetic analyses unraveled its endocytic role that is required for proper internalization of receptors controlling different signaling pathways. In this study, we analyzed the role of Awd in controlling Notch signaling during development.ResultsTo study the awd gene function we used genetic mosaic approaches to obtain cells homozygous for a loss of function allele. In awd mutant follicle cells and wing disc cells, Notch accumulates in enlarged early endosomes, resulting in defective Notch signaling. Our results demonstrate that awd function is required before γ-secretase mediated cleavage since over-expression of the constitutively active form of the Notch receptor in awd mutant follicle cells allows rescue of the signaling. By using markers of different endosomal compartments we show that Notch receptor accumulates in early endosomes in awd mutant follicle cells. A trafficking assay in living wing discs also shows that Notch accumulates in early endosomes. Importantly, constitutively active Rab5 cannot rescue the awd phenotype, suggesting that awd is required for Rab5 function in early endosome maturation.ConclusionsIn this report we demonstrate that awd is essential for Notch signaling via its endocytic role. In addition, we identify the endocytic step at which Awd function is required for Notch signaling and we obtain evidence indicating that Awd is necessary for Rab5 function. These findings provide new insights into the developmental and pathophysiological function of this important gene family.

show abstract

“…The process of proliferation and differentiation of the follicle cells is complex and under stringent control [37-39]. One critical event is the cessation of mitosis in mid-oogenesis.…”

Section: Resultsmentioning

confidence: 99%

Notch signaling during development requires the function of awd, the Drosophila homolog of human metastasis suppressor gene Nm23

et al. 2014

View full text Add to dashboard Cite

show abstract

“…In Drosophila the family is represented by a single gene, bunched/shortsighted, which is implicated in decapentaplegic (dpp) signalling in the developing eye (Treisman et al, 1995;Dobens et al, 1997Dobens et al, , 2000. The fact that Hh is responsible for controlling dpp expression in Drosophila, combined with the recent discovery that TSC-22 is down-regulated by stable Gli1 expression in rat kidney cells (Yoon et al, 2002), makes this family of particular interest in the Shh pathway.…”

Section: Igf2 Bf-2 Amh and Sfrp-2 Are Regulated By Gli1mentioning

confidence: 99%

Novel genes regulated by Sonic Hedgehog in pluripotent mesenchymal cells

et al. 2002

View full text Add to dashboard Cite

“…The 16 cell cysts are then surrounded by somatic follicle cells (FC) that originate from FC stem cells located in the middle of the germarium (Margolis and Spradling, 1995). The daughters of the FC stem cells first extend thin centripetal processes and then migrate around nascent cysts to envelop them as a monolayered epithelium, thereby separating adjacent cysts from each other (Dobens and Raftery, 2000). …”

Section: Introductionmentioning

confidence: 99%

Drosophilasosiefunctions with βH-Spectrin and actin organizers in cell migration, epithelial morphogenesis and cortical stability

2012

View full text Add to dashboard Cite

SummaryMorphogenesis in multicellular organisms requires the careful coordination of cytoskeletal elements, dynamic regulation of cell adhesion and extensive cell migration. sosie (sie) is a novel gene required in various morphogenesis processes in Drosophila oogenesis. Lack of sie interferes with normal egg chamber packaging, maintenance of epithelial integrity and control of follicle cell migration, indicating that sie is involved in controlling epithelial integrity and cell migration. For these functions sie is required both in the germ line and in the soma. Consistent with this, Sosie localizes to plasma membranes in the germ line and in the somatic follicle cells and is predicted to present an EGF-like domain on the extracellular side. Two positively charged residues, C-terminal to the predicted transmembrane domain (on the cytoplasmic side), are required for normal plasma membrane localization of Sosie. Because sie also contributes to normal cortical localization of βH-Spectrin, it appears that cortical βH-Spectrin mediates some of the functions of sosie. sie also interacts with the genes coding for the actin organizers Filamin and Profilin and, in the absence of sie function, F-actin is less well organized and nurse cells frequently fuse.

show abstract

Integration of epithelial patterning and morphogenesis inDrosophila ovarian follicle cells

Cited by 162 publications

References 77 publications

Notch signaling during development requires the function of awd, the Drosophila homolog of human metastasis suppressor gene Nm23

Notch signaling during development requires the function of awd, the Drosophila homolog of human metastasis suppressor gene Nm23

Novel genes regulated by Sonic Hedgehog in pluripotent mesenchymal cells

Drosophilasosiefunctions with βH-Spectrin and actin organizers in cell migration, epithelial morphogenesis and cortical stability

Contact Info

Product

Resources

About