Integration of data from the in vitro long-term exposure study on human endothelial cells and the in silico analysis: A case of dibutyl phthalate-induced vascular dysfunction

Stanić, Bojana; Kokai, Dunja; Tesic, Biljana; Fa, Svetlana; Nenadov, Dragana Samardzija; Pogrmić-Majkić, Kristina; Andrić, Nebojša

doi:10.1016/j.toxlet.2021.12.006

Cited by 10 publications

(2 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Exposure to DEHP has been documented to be a risk factor for cardiovascular diseases [ 25 , 26 , 49 ]. Although endothelial cells are directly exposed to circulating plasticizers, few studies have evaluated the endothelial toxicity of plasticizers, and to a lesser extent, mixture effects [ 28 , 30 , 32 , 41 , 50 ]. Considering that DEHP regulation paved the way to its substitution by other plasticizers, such as DINP and DEHT, it is necessary to evaluate their effects on the vascular system owing to the lack of data.…”

Section: Discussionmentioning

confidence: 99%

Effects of DEHP, DEHT and DINP Alone or in a Mixture on Cell Viability and Mitochondrial Metabolism of Endothelial Cells In Vitro

Poitou

Rogez-Florent

Dirninger

et al. 2022

Toxics

View full text Add to dashboard Cite

Plasticizers are chemicals in high demand, used in a wide range of commercial products. Human are exposed through multiple pathways, from numerous sources, to multiple plasticizers. This is a matter of concern, as it may contribute to adverse health effects. The vascular system carries plasticizers throughout the body and therefore can interact with the endothelium. The aim of the study was to evaluate the in vitro toxicity on endothelial cells by considering the individual and the mixture effects of bis-(2-ethylhexyl) phthalate (DEHP), diisononyl phthalate (DINP) or bis-(2-ethylhexyl) terephthalate (DEHT). In this study, their cytotoxicity on HMEC-1 cells was evaluated on cell function (viability, cell counting, total glutathione and intracellular adenosines) and mitochondrial function (mitochondrial respiration). Results showed cellular physiological perturbations induced with all the condition tested, excepted for DEHT. Plasticizers induced a cytotoxicity by targeting mitochondrial respiration, depleting mitochondrial ATP production and increasing glycolytic metabolism. Additionally, delayed effects were observed between the cellular and the mitochondrial parameters. These results suggest that endothelial cells could go through a metabolic adaptation to face plasticizer-induced cellular stress, to effectively maintain their cellular processes. This study provides additional information on the adverse effects of plasticizers on endothelial cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of DEHP, DEHT and DINP Alone or in a Mixture on Cell Viability and Mitochondrial Metabolism of Endothelial Cells In Vitro

Poitou

Rogez-Florent

Dirninger

et al. 2022

Toxics

View full text Add to dashboard Cite

show abstract

“…Although DBP predominantly affects the endocrine and reproductive systems, it can also have an impact on the vasculature system's functionality. Stanic et al [71] integrated the toxicogenomic data of human vascular endothelial cells (ECs) in order to infer pathways, molecular activities, biological processes, and human diseases related to DBP exposure. Nine genes, including six members of the integrin family, VCAM1, ICAM1, and MMP2, were found to be affected by DBP exposure, according to these authors, who concluded that changes in the DBP-affected genes could affect the extracellular matrix and the binding of molecules and cells to ECs, changing cell adhesion and migration.…”

Section: Plasticizers and Phthalatesmentioning

confidence: 99%

State of the Art of Genomic Technology in Toxicology: A Review

Recio-Vega

Facio-Campos

Hernández-González

et al. 2023

IJMS

View full text Add to dashboard Cite

The rapid growth of genomics techniques has revolutionized and impacted, greatly and positively, the knowledge of toxicology, ushering it into a “new era”: the era of genomic technology (GT). This great advance permits us to analyze the whole genome, to know the gene response to toxicants and environmental stressors, and to determine the specific profiles of gene expression, among many other approaches. The aim of this work was to compile and narrate the recent research on GT during the last 2 years (2020–2022). A literature search was managed using the PubMed and Medscape interfaces on the Medline database. Relevant articles published in peer-reviewed journals were retrieved and their main results and conclusions are mentioned briefly. It is quite important to form a multidisciplinary taskforce on GT with the aim of designing and implementing a comprehensive, collaborative, and a strategic work plan, prioritizing and assessing the most relevant diseases, so as to decrease human morbimortality due to exposure to environmental chemicals and stressors.

show abstract

Dibutyl phthalate promotes angiogenesis in EA.hy926 cells through estrogen receptor-dependent activation of ERK1/2, PI3K-Akt, and NO signaling pathways

Kokai

Stanić

Tesic

et al. 2022

Chemico-Biological Interactions

View full text Add to dashboard Cite

Integration of data from the in vitro long-term exposure study on human endothelial cells and the in silico analysis: A case of dibutyl phthalate-induced vascular dysfunction

Cited by 10 publications

References 42 publications

Effects of DEHP, DEHT and DINP Alone or in a Mixture on Cell Viability and Mitochondrial Metabolism of Endothelial Cells In Vitro

Effects of DEHP, DEHT and DINP Alone or in a Mixture on Cell Viability and Mitochondrial Metabolism of Endothelial Cells In Vitro

State of the Art of Genomic Technology in Toxicology: A Review

Dibutyl phthalate promotes angiogenesis in EA.hy926 cells through estrogen receptor-dependent activation of ERK1/2, PI3K-Akt, and NO signaling pathways

Contact Info

Product

Resources

About