Integration of continuous-high cell density-fed-batch fermentation for Aurantiochytrium limacinum for simultaneous high biomass, lipids and docosahexaenoic acid production

Pawar, Pratik R.; Lali, Arvind; Prakash, Gunjan

doi:10.1016/j.biortech.2020.124636

Cited by 20 publications

(7 citation statements)

References 22 publications

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“…The fed-batch process is widely accepted as a common approach for obtaining a high final product concentration with an acceptable yield. In fed-batch fermentation, a wide range of feeding programs has been proposed for specific operational purposes [ 100 ]. With a suitable substrate feeding program, essential nutrients (typically the carbon source) are maintained at an optimal concentration such that a high product yield and productivity are obtained [ 101 ].…”

Section: Fundamental Elements In the Fermentation Process Developmentmentioning

confidence: 99%

The outlooks and key challenges in renewable biomass feedstock utilization for value-added platform chemical via bioprocesses

Khunnonkwao,

Thitiprasert,

Jaiaue

et al. 2024

Heliyon

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Section: Fundamental Elements In the Fermentation Process Developmentmentioning

confidence: 99%

The outlooks and key challenges in renewable biomass feedstock utilization for value-added platform chemical via bioprocesses

Khunnonkwao,

Thitiprasert,

Jaiaue

et al. 2024

Heliyon

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“…It has been proven that fed batch cultures are very effective, as they enable a significant increase in the biomass of the microorganisms and influence the achievement of a high lipid content in the cells [84]. This type of culture variant was used in the production of lipids and DHA by the microalgae Crypthecodinium cohnii and Aurantochytrium sp.…”

Section: Production Of Bio-oilmentioning

confidence: 99%

Waste Glycerol in the Biosynthesis of Liquid Fuels and Other Value-Added Products – a Review

Kazimierowicz,

Dębowski,

Zieliński

et al. 2024

Preprint

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Waste glycerol can be subjected to various processing operations, including purification and re-fining, to obtain glycerol of appropriate purity. In addition, alternative methods of utilising waste glycerol are also being sought, e.g. by converting it into other valuable chemical products or biofuels. Therefore, various technologies are being developed to utilise this type of waste effectively and sustainably. The production of value-added products from waste glycerol is an important issue that determines the improvement of the economic viability of biofuel production and corresponds to the model of a waste-free and emission-free circular economy. This paper characterised the mechanisms and evaluated the efficiency of existing methods of microbiological utilisation of waste glycerol into liquid biofuels, including biodiesel, bioethanol and biobutanol, and identified further directions for the production of value-added products. In addition, a bibliographical analysis of publications re-lated to the production of liquid fuels and economically valuable products from glycerol was carried out, the progress of research and application work was assessed and directions for future research were identified.

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“…ATCC PRA-276 [17], and waste oil for Aurantiochytrium limacinum SR21 [18]. Solid waste from food processing, cafeteria food waste [19], poultry waste [20] and brewery spent yeast [21] have also been demonstrated to be useful but an hydrolysis pretreatment was required before they could be used. However, only a limited range of information is available on the applicability of solid-state waste for cultivating thraustochytrids to produce PUFAs.…”

Section: Introductionmentioning

confidence: 99%

Valorization of Solid Food Waste as a Source of Polyunsaturated Fatty Acids Using Aurantiochytrium sp. L3W

Suenaga

Nakai

Umehara

et al. 2023

Waste Biomass Valor

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Purpose: This study aimed at valorizing solid food waste containing docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA).Methods: Aurantiochytrium sp. L3W that produces DHA and EPA was cultivated on eight types of solid food waste: sake lees (SL), crown daisy, Japanese mustard spinach (JMS), soy sauce residue, lemon peel (LP), orange peel, grape skin, and Hiroshimana old pickle (HOP). The biomass mixture of the remaining food waste and strain L3W was analyzed for DHA and EPA. To characterize the types of food waste, the leachability of dissolved organic carbon (DOC) and dissolved nitrogen (DN) were compared.Results: The strain L3W grew on both pasteurized and unsterilized food waste such as SL and JMS. Elution of DOC and DN from the food waste might be a factor affecting the growth of strain L3W.However, the strain L3W might utilize solid-state organic compounds in JMS. Despite the unsterile conditions, the biomass mixture of SL contained both DHA and EPA, whereas DHA was found in the biomass mixtures of JMS, LP and HOP, thereby con rming the valorization of these types of solid food waste. Unsterile mass cultivation of the strain L3W using SL and HOP in a 200 L tank also produced a biomass mixture containing 12.6 mg-DHA/g and 0.217 mg-EPA/g. These DHA and EPA contents were 1500-times and 37-times higher, respectively, than that in commercial poultry feed, indicating that these mixtures could be used as an additive in poultry feed. Statement Of NoveltyThe demand for docosahexaenoic and eicosapentaenoic acids (DHA and EPA) from sh oil has been increasing because of its bene cial use in the aquaculture and food industries but sh oil resources are now becoming unsustainable. Therefore, we have focused on using the heterotrophic microorganism, Aurantiochytrium sp. L3W, to produce DHA and EPA. The present study demonstrated that solid food waste can be used for culturing Aurantiochytrium sp. L3W without sterilization and hydrolysis pretreatments and provided evidence that the strain L3W may utilize solid-state carbon and nitrogen. We obtained biomass mixtures consisting of the remaining food waste and analyzed them for DHA and EPA. The DHA and EPA contents were 1500-times and 37-times higher, respectively, than those in commercial poultry feed. This is the rst report on valorizing solid food waste as a source of PUFAs that are potentially usable as an additive for poultry feed to produce products enriched with PUFAs, such as eggs.

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Integration of continuous-high cell density-fed-batch fermentation for Aurantiochytrium limacinum for simultaneous high biomass, lipids and docosahexaenoic acid production

Cited by 20 publications

References 22 publications

The outlooks and key challenges in renewable biomass feedstock utilization for value-added platform chemical via bioprocesses

The outlooks and key challenges in renewable biomass feedstock utilization for value-added platform chemical via bioprocesses

Waste Glycerol in the Biosynthesis of Liquid Fuels and Other Value-Added Products – a Review

Valorization of Solid Food Waste as a Source of Polyunsaturated Fatty Acids Using Aurantiochytrium sp. L3W

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