Integration of biocontrol yeast and thiabendazole protects stored apples from fungicide sensitive and resistant isolates of Botrytis cinerea

Lima, Giuseppe; Curtis, Filippo De; Piedimonte, D.; Spina, Annamaria; Cicco, V. De

doi:10.1016/j.postharvbio.2006.01.017

Cited by 55 publications

(43 citation statements)

References 24 publications

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“…The potential of including BCAs with mefenoxam can be in form of rotations with mefenoxam to reduce the frequency of fungicide applications for a more environmentally friendly, long lasting, consistent, and effective control of P. capsici. Similar results on BCA have been reported in other studies [50][51][52][53][54].…”

Section: Discussionsupporting

confidence: 91%

Evaluation of Selected Bacterial Endophytes for Biocontrol Potential against Phytophthora Blight of Bell Pepper (Capsicum annuum L.)

A¹,

Mt²

2017

J Plant Pathol Microbiol

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Phytophthora blight, caused by Phytophthora capsici, is the most destructive disease of bell pepper in the United States. The effectiveness of current management strategies is limited by the long-term survival of oospores, wide host range, aggressive fungicide-resistant isolates, and lack of resistant cultivars with acceptable agronomic traits. Biological control is a viable alternative, and the use of endophytic microorganisms as biological control agents (BCA) has attracted widespread attention because they colonize the same ecological niches as plant pathogens. Seven isolates of bacterial endophytes in three genera Serratia (B17B), Enterobacter (E), and Bacillus (IMC8, Y, Ps, Psl and Prt), isolated from papaya, snap bean and flowering dogwood were evaluated for effects on P. capsici mycelial growth in vitro, and on disease severity and plant growth in greenhouse environment. All isolates significantly inhibited the mycelial growth of P. capsici with Ps, Psl and Prt having superior inhibitory effects. Seed treatments with Ps, Psl and Prt followed by plant inoculation with P. capsici reduced disease severity, and significantly increased plant shoot height, fresh weight, and fruit yield (number and weight) with Ps and Prt being slightly superior to Psl. Compatibility tests between isolates showed that only B17B and Y were compatible with each other. Sensitivity tests to different levels of mefenoxam (Ridomil Gold SL) showed that Ps, Psl and Prt were tolerant to the fungicide, while P. capsici was highly sensitive. Thus, Ps, Psl and Prt can be used in rotation with mefenoxam to reduce the frequency of fungicide usage for a more environmentally friendly, long lasting, consistent, and effective control of phytophthora blight. Overall, all isolates tested are potential BCAs against P. capsici, but more greenhouse and field studies are required to confirm in vitro results for all isolates that showed good potential in in vitro studies.

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Section: Discussionsupporting

confidence: 91%

Evaluation of Selected Bacterial Endophytes for Biocontrol Potential against Phytophthora Blight of Bell Pepper (Capsicum annuum L.)

A¹,

Mt²

2017

J Plant Pathol Microbiol

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“…Thus, modified storage conditions, physical and chemical treatments have been used in combination with biocontrol agents and their additive or synergic effect have increased the level of disease control (MARI; NERI; BERTOLINI, 2007;CONWAY, 2010). Integrating the antagonistic Cryptococcus laurentii with 104 ppm of thiabendazole improved the control of grey mould caused by Botrytis cinerea on apples (LIMA et al, 2006). Similarly, the application of the same antagonist in combination with 25 ppm imazalil or 50 ppm kresoximmethyl resulted in less decay caused by Alternaria alternata and Monilinia fructicola (compared with separate applications) on jujube fruits stored in a controlled atmosphere (QIN; TIAN, 2004).…”

Section: Integration Of Biological Control With Other Methodsmentioning

confidence: 99%

“…The main strategy includes the use of fungicides (EDWARDS, et al, 2001) which is also considered the primary means by which postharvest diseases are controlled (SPADARO; GULLINO, 2004;KORSTEN, 2006;DAL BELLO et al, 2008). Besides, currently many reasons -public perception that pesticides are harmful to human health and the environment (JANISIEWICZ; KORSTEN, 2002), limited efficacy for the development of pathogen resistance (LIMA et al, 2006;BRODERS et al, 2007), and the public demand for produce food free of synthetic pesticides (SIPICZKI, 2006) -have been requesting to replace the synthetic chemicals use to a safer and cleaner alternative approach. In this background, biological control using antagonistic microorganisms has been an emergent alternative to efficiently manage storage fungi and mycotoxins production and hence, reducing the use of chemical compounds KORSTEN, 2002;SPADARO;GULLINO 2004;KORSTEN, 2006;VELMOUROUGANE et al, 2011).…”

Section: Biological Controlmentioning

confidence: 99%

Biological control of mycotoxin-producing molds

et al. 2012

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Mycotoxins are produced by the secondary metabolism of many fungi and can be found in almost 25% of the world's agricultural commodities. These compounds are toxic to humans, animals, and plants and therefore, efforts should be made to avoid mycotoxin contamination in food and feed. Besides, up to 25% of all harvested fruits and vegetables are lost due to storage molds and/ or mycotoxin contamination and many methods have been applied to mitigate these issues, but most of them rely on the use of fungicides. Although chemicals are often the first defensive line against mycotoxigenic fungi, the indiscriminate use of fungicides are awakening the public perception due to their noxious effects on the environment and human/animal health. Thus, there is an increasing public pressure for a safer and eco-friendly alternative to control these organisms. In this background, biological control using microbial antagonists such as bacteria, fungi and yeasts have been shown to be a feasible substitute to reduce the use of chemical compounds. Despite of the positive findings using the biocontrol agents only a few products have been registered and are commercially available to control mycotoxin-producing fungi. This review brings about the up-to-date biological control strategies to prevent or reduce harvested commodity damages caused by storage fungi and the contamination of food and feed by mycotoxins.Index terms: Biocontrol, microbial antagonists, postharvest decay, food safety. RESUMOAs micotoxinas são produzidas pelo metabolismo secundário de várias espécies de fungos e podem ser encontradas em quase 25% das commodities agrícolas. Esses compostos são tóxicos a humanos, animais e plantas e, portanto, esforços para evitar a contaminação de micotoxinas em alimentos e rações devem ser feitos. Além disso, até 25% das frutas e legumes em pós-colheita são perdidos em decorrência do ataque de fungos de armazenamento e/ou contaminações por micotoxinas. Vários métodos têm sido aplicados para mitigar os problemas de micotoxinas, mas a maioria deles se baseia no uso de fungicidas. Embora os produtos químicos sejam, muitas vezes, a primeira estratégia de defesa contra fungos micotoxigênicos, o uso indiscriminado de fungicidas vem despertando a percepção pública, em razão de seus efeitos nocivos sobre o meio ambiente e à saúde humana/animal. Assim, existe uma crescente pressão pública em busca de alternativas mais segura e não nocivas ao meio ambiente para controlar estes organismos. Nesse contexto, o controle biológico utilizando antagonistas microbianos, tais como bactérias, fungos e leveduras têm mostrado ser um substituto viável para reduzir a utilização de produtos químicos. Apesar dos resultados positivos, usando os agentes de controle biológico, poucos produtos apenas foram registrados e estão comercialmente disponíveis para controlar fungos produtores de micotoxinas. Esta revisão traz estratégias de controle biológico para evitar ou reduzir danos em commodities agrícolas causadas por fungos de armazenamento e a contaminaç...

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“…Isolating novel strains harboring antifungal properties from different habitats is of great importance for postharvest disease control 4,6 . Some yeasts exhibit antagonistic behaviors toward other microorganisms such as yeasts and molds by producing killer toxins.…”

Section: Resultsmentioning

confidence: 99%

“…Nevertheless, the use of synthetic compounds has been progressively restrained because of consumers' no longer want to see toxic or chemical residues in their foods 3 . Furthermore, the increasing occurrence of phytopathogens that are resistant to chemical agents encourages the necessitate to seek for different alternatives for disease control [4][5][6] . It is well known that members of certain groups of microorganisms have the ability to produce and secrete extracellular compounds that display antagonistic behaviors against others 7 .…”

Section: Introductionmentioning

confidence: 99%