Integration of a microfluidic chip with a size-based cell bandpass filter for reliable isolation of single cells

Kim, Hojin; Lee, Sang‐Hyun; Lee, Jae‐Hyung; Kim, Joonwon

doi:10.1039/c5lc00904a

Cited by 34 publications

(25 citation statements)

References 27 publications

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“…The herringbone structure, designed and further improved herein, was tested in conjunction with an array of trapping structures with a goal to enhance trapping efficiency [8,9]. The following equation represents trapping efficiency Eq.…”

Section: Application Of the Device To Cell Trappingmentioning

confidence: 99%

Manipulation of cells’ position across a microfluidic channel using a series of continuously varying herringbone structures

Jung

Hyun

Choi

et al. 2017

Micro and Nano Syst Lett

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Controlling cells' movement is an important technique in biological analysis that is performed within a microfluidic system. Many external forces are utilized for manipulation of cells, including their position in the channel. These forces can effectively control cells in a desired manner. Most of techniques used to manipulate cells require sophisticated set-ups and equipment to generate desired effect. The exception to this is the use of hydrodynamic force. In this study, a series of continuously varying herringbone structures is proposed for positioning cells in a microfluidic channel using hydrodynamic force. This structure was experimentally developed by changing parameters, such as the length of the herringbone's apex, the length of the herringbone's base and the ratio of the height of the flat channel to the height of the herringbone structure. Results of this study, have demonstrated that the length of the herringbone's apex and the ratio of the heights of the flat channel and the herringbone structure were crucial parameters influencing positioning of cells at 100 μl/h flow rate. The final design was fixed at 170 and 80 μm for the length of herringbone's apex and the length of herringbone's base, respectively. The average position of cells in this device was 34 μm away from the side wall in a 200 μm wide channel. Finally, to substantiate a practical application of the herringbone structure for positioning, cells were randomly introduced into a microfluidic device, containing an array of trapping structures together with a series of herringbone structures along the channel. The cells were moved toward the trapping structure by the herringbone structure and the trapping efficiency was increased. Therefore, it is anticipated that this device will be utilized to continuously control cells' position without application of external forces.

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Section: Application Of the Device To Cell Trappingmentioning

confidence: 99%

Manipulation of cells’ position across a microfluidic channel using a series of continuously varying herringbone structures

Jung

Hyun

Choi

et al. 2017

Micro and Nano Syst Lett

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“…Previous works characterizing hydrodynamic cell-trapping systems have reported a trapping efficiency-defined as the percentage of traps filled after injection of cells-between 75% and 99%. These methods have been used to capture multiple cell types [14] and, in some cases, to localize rare cells [15]. They also proved to be valuable tools for investigation of single-cell behaviors.…”

Section: Of 14mentioning

confidence: 99%

Selective Retrieval of Individual Cells from Microfluidic Arrays Combining Dielectrophoretic Force and Directed Hydrodynamic Flow

et al. 2020

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Hydrodynamic-based microfluidic platforms enable single-cell arraying and analysis over time. Despite the advantages of established microfluidic systems, long-term analysis and proliferation of cells selected in such devices require off-chip recovery of cells as well as an investigation of on-chip analysis on cell phenotype, requirements still largely unmet. Here, we introduce a device for single-cell isolation, selective retrieval and off-chip recovery. To this end, singularly addressable three-dimensional electrodes are embedded within a microfluidic channel, allowing the selective release of single cells from their trapping site through application of a negative dielectrophoretic (DEP) force. Selective capture and release are carried out in standard culture medium and cells can be subsequently mitigated towards a recovery well using micro-engineered hybrid SU-8/PDMS pneumatic valves. Importantly, transcriptional analysis of recovered cells revealed only marginal alteration of their molecular profile upon DEP application, underscored by minor transcriptional changes induced upon injection into the microfluidic device. Therefore, the established microfluidic system combining targeted DEP manipulation with downstream hydrodynamic coordination of single cells provides a powerful means to handle and manipulate individual cells within one device.

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“…Microchips designed based on this technology differentiate the CTCs from normal blood cells regarding their different sizes (Kim et al, 2015). As an advantage, in this method the needs for specific biomarkers are obviated, while tumor cell size heterogeneity is considered a challenging feature which can fail the purification of small size tumor cells.…”

Section: Microchip Mediated Ctc Isolation Using Size-based Technologymentioning

confidence: 99%

Microchips and their Significance in Isolation of Circulating Tumor Cells and Monitoring of Cancers

Sahmani

Vatanmakanian²,

Goudarzi³

et al. 2016

Asian Pacific Journal of Cancer Prevention

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Integration of a microfluidic chip with a size-based cell bandpass filter for reliable isolation of single cells

Abstract: We report a simple, efficient microfluidic array system for reliable isolation of cells. A microfluidic array chip, integrated with a size-based cell bandpass filter, provides the unprecedented capability of organizing single cells from a population containing a wide distribution of sizes.

Cited by 34 publications

References 27 publications

Manipulation of cells’ position across a microfluidic channel using a series of continuously varying herringbone structures

Manipulation of cells’ position across a microfluidic channel using a series of continuously varying herringbone structures

Selective Retrieval of Individual Cells from Microfluidic Arrays Combining Dielectrophoretic Force and Directed Hydrodynamic Flow

Microchips and their Significance in Isolation of Circulating Tumor Cells and Monitoring of Cancers

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