Integration of a contactless conductivity detector into a commercial capillary cassette

Vuorinen, Pasi S; Jussila, Matti; Sirén, Heli M. M.; Palonen, Sami; Riekkola, Marja‐Liisa

doi:10.1016/s0021-9673(03)00047-5

Cited by 27 publications

(31 citation statements)

References 29 publications

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“…Fracassi da Silva et al [25] presented a miniaturized detector incorporated on a printed circuit board with dimensions of only 18 Â 18 mm. Hilder et al [37], Vuorinen et al [38] and Macka et al [39] designed miniaturized versions of the CCD cell for easy incorporation into existing commercial instruments. Kappes et al [40] incorporated a CCD into a purpose built portable CE instrument.…”

Section: Instrumental Developmentsmentioning

confidence: 99%

“…Thanks to its good separation efficiency, low specific conductivity but considerably high ionic strength, a buffer based on 2-(N-morpholino)ethanesulfonic acid and histidine (MES/His) has been widely used for the determination of alkali and alkaline earth cations and ammonium ions [19,20,27,32,33,36,38,40,41,47,48]. An example of such a separation is given in Figure 5.…”

Section: Inorganic Ionsmentioning

confidence: 99%

“…The determination of fructose, glucose, galactose and sucrose in soft drinks and sugarcane spirit was demonstrated. Vuorinen et al [38] demonstrated a CE-CCD determination of catecholamines. It is also emerging that CCD can be useful for the detection of larger biomolecules.…”

Section: Organic Ionsmentioning

confidence: 99%

See 2 more Smart Citations

Contactless Conductivity Detection in Capillary Electrophoresis: A Review

2004

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The popularity of contactless conductivity detection in capillary electrophoresis has been growing steadily over the last few years. Improvements have been made in the design of the detector in order to facilitate its handling, to allow easy incorporation into available instruments or to achieve higher sensitivity. The understanding of its fundamental working principles has been advanced and the detection approach has also been transferred to lab-on-chip devices. The range of applications has been extended greatly from the initial work on small inorganic ions to include organic species and biomolecules. Concurrent determination of cations and anions by dual injection from opposite ends has been demonstrated as well as sample introduction by using flow-injection systems for easy automation of the process.

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Section: Instrumental Developmentsmentioning

confidence: 99%

Section: Inorganic Ionsmentioning

confidence: 99%

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Contactless Conductivity Detection in Capillary Electrophoresis: A Review

2004

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“…Generally the systems consist of two parts, a detector cell and a separate case containing the associated electronics. Macka et al [17] and Vuorinen et al [18] described very small detector cells for easy placement into existing commercial CE instruments which were not originally designed for retrofitting of alternative detectors. Tanyanyiwa et al [19,20] described a system which was constructed for high signal to noise ratio by using an elevated excitation voltage and a detector cell which includes a pre-amplifier.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Different Contactless Conductivity Detectors for the Determination of Small Inorganic Ions by Capillary Electrophoresis

et al. 2006

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The analytical performance parameters of four different contactless conductivity detectors for capillary electrophoresis were determined. These detectors were designed either with a miniature cell for versatility, with high voltage excitation for high sensitivity or with a battery power supply for field application. One of the units is commercially available. The plate numbers and reproducibility of peak areas (typically about 2%) were very similar, indicating that these parameters are generally limited by the separation and injection procedures rather than the detectors. The linear dynamic ranges are better than 2 orders of magnitude for all detectors and the correlation coefficients were also almost identical. Significant differences were found with regard to the detection limits. For the detector with a miniature cell, which lacks an in-cell amplifier, the detection limit was typically 1.5 mM for the inorganic ions tested (K þ , Ca 2þ , Na þ , Mg 2þ , Li þ , Cl À , NO 3 À and SO 4 2À ), while with the other 3 detectors this parameter was as low as about 0.1 mM. The use of buffer solutions with relatively high background conductivity was found to lead to detection limits which were up to one order of magnitude higher. The extent of deterioration of this parameter with buffer conductivity was found to be related to the excitation voltage and was most pronounced for the high voltage detector.

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“…Contactless electrical detectors are universal detectors suitable for all manner of compounds. 4 Over the past decade, many different approaches5-7 have been proposed for integrating electrodes within microfluidic devices in such a way that they do not make contact with the solution, but are located at a very small distance from the capillary such that their detection sensitivity is enhanced. Recently, various optical detection methods have been proposed in which optical waveguides such as Su-8/spin-on-glass (SOG)8 or optical fibers9 are buried beside the separation channel.…”

Section: Introductionmentioning

confidence: 99%