Integration and validation of the ex vivo human placenta perfusion model

Abstract

Based on the assessment of multiple quality control measurements, the described method of a closed human ex-vivo placenta perfusion model was validated. The success rate (38%) was more than twice the success rate reported in literature (15%).

Larger First-Trimester Placental Volumetric Parameters Are Associated With Lower Pressure and More Flow-Mediated Vasodilation of the Fetoplacental Vasculature After Delivery

“…On the one hand it has been demonstrated that placentas after vaginal delivery show increased oxidative stress and inflammatory cytokines on both gene-and protein levels (Cindrova-Davies et al, 2007). On the other hand, multiple studies showed no difference in placental barrier function during ex vivo perfusion for delivery mode (Mathiesen et al, 2010;Conings et al, 2017). Lastly, identified correlations should be cautiously interpreted due to the small sample size of the study, which also hampered correction for multiple testing.…”

Translocation of (ultra)fine particles and nanoparticles across the placenta; a systematic review on the evidence of in vitro, ex vivo, and in vivo studies

“…Ex vivo perfusion of human term placentae is used as a surrogate to study the transplacental transport of NPs. It is considered as the “gold standard” among currently available translocation models as it preserves the structural complexity of a full-term placenta and resembles its dynamic environment, enabling to study ex vivo transplacental NP passage without harming the mother and/or fetus [ 126 ]. Nonetheless, perfusion studies (i) predominantly use full-term placentae and, hence, do not allow to estimate NP transfer during earlier and more vulnerable stages of pregnancy, (ii) are limited to a few hours (4–8 h) of perfusion due to tissue degradation, which is insufficient to observe chronic effects, (iii) have a low success rate of perfusion (i.e.…”

“…Nonetheless, perfusion studies (i) predominantly use full-term placentae and, hence, do not allow to estimate NP transfer during earlier and more vulnerable stages of pregnancy, (ii) are limited to a few hours (4–8 h) of perfusion due to tissue degradation, which is insufficient to observe chronic effects, (iii) have a low success rate of perfusion (i.e. , 30%), and (iv) are time-consuming [ 126 , 127 ].…”

Placental macrophages: Origin, heterogeneity, function and role in pregnancy-associated infections

“…Second, their location in a complex and rapidly evolving tissue has favored in situ investigations, which precluded functional studies. An ex vivo placental perfusion assay might be an alternative to study placental macrophages in their natural microenvironment [ 12 , 13 ]. However, despite its initial description some 50 years ago, placental perfusion assay has been mainly limited to pharmacological investigations [ 14 ].…”