Integration analysis of transcriptomics and proteomics reveals the inhibitory effect of carvacrol on Alternaria alternata by interfering with carbohydrate metabolism

Zhao, Lunaike; Wang, Junjie; Zhang, Haiyu; Zhou, Yueli; Wang, Cong; Wang, Peng; Wei, Zhaojun; Li, Huanhuan; Ding, Ye

doi:10.1016/j.postharvbio.2024.112928

Cited by 2 publications

(1 citation statement)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the magnolol treatment of A. alternata, genes that affect global regulators of carbon and nitrogen utilization (such as CreA and NmrA), the mitogen-activated protein kinase (MAPK) signaling pathway, and mycotoxin biosynthesis were also found to be downregulated [21]. As per our two previous studies, transcriptome analysis showed that a low concentration of CVR (0.06 µL/mL) had an impact on cell wall-related genes in A. alternata [8], and the integration analysis of transcriptomics and proteomics further suggested that a low concentration of CVR inhibited A. alternata in vitro by interfering with carbohydrate metabolism [30]. This, in turn, affected the growth of the newly forming mycelium's cell wall, which plays a critical role in the differentiation of the infection structure.…”

Section: Introductionsupporting

confidence: 70%

Inhibitory Effect and Mechanism of Carvacrol against Black Mold Disease Agent Alternaria alternata in Goji Berries

Wang,

Zhou,

Wang

et al. 2024

JoF

Self Cite

View full text Add to dashboard Cite

Alternaria alternata, as a main decay fungus of goji berry, can produce mycotoxins such as alternariol (AOH), alternariol monomethyl ether (AME), and tenuazonic acid (TeA). Carvacrol (CVR) has exhibited a broad-spectrum antifungal activity in vitro. We assumed that CVR can also be applied to control Alternaria rot on goji berries and mycotoxins produced by the pathogens. To investigate whether CVR impacts the accumulation of mycotoxins and cell membrane damage of A. alternata, the antifungal activity of CVR on the fungal growth and mycotoxin production was evaluated in this study. The results showed that the minimum inhibitory concentration (MIC) of CVR against A. alternata was 0.12 µL/mL. Meanwhile, the destruction of plasma membrane integrity, cytoplasmic leakage, intracellular oxidative damage, and inhibitory effect in vivo were also observed in A. alternata treated with CVR. Moreover, CVR significantly reduced the accumulation of AOH, AME, and TeA. Transcriptomic profiling was performed by means of comparative RNA-Seq analysis to research the gene expression level of A. alternata, which attested to significant changes in nitrogen metabolism, carbon utilization, fatty acid oxidation, and antioxidant enzymes in CVR-treated A. alternata. This study suggests a new understanding of the molecular mechanism of response to CVR treatment in A. alternata, indicating that CVR is a novel antifungal agent with the potential to be applied to various fungi.

show abstract

Section: Introductionsupporting

confidence: 70%