Integrating UPLC-Q-Exactive Orbitrap/MS, network pharmacology and experimental validation to reveal the potential mechanism of Tibetan medicine Rhodiola granules in improving myocardial ischemia-reperfusion injury

Xing, Nan; Qin, Jing; Ren, Dongsheng; Du, Qinyun; Li, Yuying; Mi, Jiao; Zhang, Fengming; Ai, Li; Zhang, Sanyin; Zhang, Yi; Wang, Shaohui

doi:10.1016/j.jep.2023.116572

Cited by 16 publications

(1 citation statement)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The above results showed that, in the state of MI, these components remained in the rats' bodies for a long time, the exposure was higher, and the clearance was lower in general. Studies have shown that RC may synergistically inhibit myocardial apoptosis and improve MI-reperfusion injury through the anti-inflammatory regulation of energy metabolism and oxidative stress, which may be related to the HIF-1/VEGF/PI3K-Akt signaling pathway [32]. Further research found that the systemic exposure to salidroside in hypoxia increased significantly, which may be related to the weakening of liver metabolism under the disease state [33].…”

Section: Discussionmentioning

confidence: 95%

Pharmacokinetic–Pharmacodynamic Correlation Analysis of Rhodiola crenulata in Rats with Myocardial Ischemia

Jia,

Zou,

Xie

et al. 2024

Pharmaceuticals

View full text Add to dashboard Cite

The pharmacokinetics (PK) of Rhodiola crenulata in rats were studied, and pharmacokinetic–pharmacodynamic (PK-PD) correlation analysis was performed to elucidate their time–concentration–effect relationship. The myocardial ischemia model was made with pituitrin. Rats were divided into sham operation, sham operation administration, model, and model administration groups (SG, SDG, MG, and MDG, respectively; n = 6). Blood was collected from the fundus venous plexus at different time points after oral administration. The HPLC-QQQ-MS/MS method was established for the quantification of five components of Rhodiola crenulata. CK, HBDH, SOD, LDH, and AST at different time points were detected via an automatic biochemical analyzer. DAS software was used to analyze PK parameters and PK-PD correlation. The myocardial ischemia model was established successfully. There were significant differences in the PK parameters (AUC0–, AUC0–∞, Cmax) in MDG when compared with SDG. Two PD indicators, CK and HBDH, conforming to the sigmoid-Emax model, had high correlation with the five components, which indicated a delay in the pharmacological effect relative to the drug concentration in plasma. The difference in the PK parameters between modeled and normal rats was studied, and the time–concentration–effect of composition and effect indicators were investigated. This study can provide reference for the rational clinical application of Rhodiola crenulata and for related studies of other anti-myocardial ischemia drugs.

show abstract

Section: Discussionmentioning

confidence: 95%

Pharmacokinetic–Pharmacodynamic Correlation Analysis of Rhodiola crenulata in Rats with Myocardial Ischemia

Jia,

Zou,

Xie

et al. 2024

Pharmaceuticals

View full text Add to dashboard Cite

show abstract

The Chemical Profile and the Quantification of the Traditional Chinese Medicine Formula Granules Erchen Decoction Analyzed by Ultra‐High Performance Liquid Chromatography‐Q‐Exactive Orbitrap Tandem Mass Spectrometry Method

Lai,

Ning,

Liu

et al. 2024

Separation Science Plus

View full text Add to dashboard Cite

Traditional Chinese medicine formula granules (TCMFGs) are the advanced dosage forms of the single traditional Chinese herbal decoction pieces, which are widely used for clinical treatment in China. With the clinical application of TCMFG, Erchen decoction (ECD), a classic traditional Chinese medicine (TCM) formula treating phlegm–dampness syndrome, is treated as TCM formula granules instead of herb decoction pieces. However, the chemical profiles and chemical consistency between TCM formula granules ECD and herbal decoction pieces are still unclear. To clarify the chemical profiles in TCM formula granules ECD, the ultra‐high performance liquid chromatography‐Q‐Exactive Orbitrap tandem mass spectrometry technology was used to characterize the chemical profiles of TCM formula granules ECD, and nine characteristic chemical components were further determined. Our exciting finding indicated that a total of 64 components were quantified and glycyrrhizic acid, hesperidin, liquiritigenin, and nobiletin were found to be the major compounds in TCM formula granules ECD and might be the pharmacodynamic material basis of ECD for phlegm–dampness syndrome treatment. The study is to explore the chemical profile and the major compounds quantitatively analysis of TCM formula granules ECD for the first time, which provides a valuable knowledge for the quality control and clinical application on TCM formula granules ECD.

show abstract

Exploring the active components and potential mechanisms of Alpiniae oxyphyllae Fructus in treating diabetes mellitus with depression by UPLC-Q-Exactive Orbitrap/MS, network pharmacology and molecular docking

Qi,

Zhou,

Zhang

et al. 2024

Metab Brain Dis

View full text Add to dashboard Cite

Integrating UPLC-Q-Exactive Orbitrap/MS, network pharmacology and experimental validation to reveal the potential mechanism of Tibetan medicine Rhodiola granules in improving myocardial ischemia-reperfusion injury

Cited by 16 publications

References 54 publications

Pharmacokinetic–Pharmacodynamic Correlation Analysis of Rhodiola crenulata in Rats with Myocardial Ischemia

Pharmacokinetic–Pharmacodynamic Correlation Analysis of Rhodiola crenulata in Rats with Myocardial Ischemia

The Chemical Profile and the Quantification of the Traditional Chinese Medicine Formula Granules Erchen Decoction Analyzed by Ultra‐High Performance Liquid Chromatography‐Q‐Exactive Orbitrap Tandem Mass Spectrometry Method

Exploring the active components and potential mechanisms of Alpiniae oxyphyllae Fructus in treating diabetes mellitus with depression by UPLC-Q-Exactive Orbitrap/MS, network pharmacology and molecular docking

Contact Info

Product

Resources

About