Integrating transcriptome and metabolome analyses of the response to cold stress in pumpkin (Cucurbita maxima)

Li, Fengmei; Lu, Xiaoxuan; Duan, Pengfei; Liang, Yanjiao; Cui, Jian

doi:10.1371/journal.pone.0249108

Cited by 16 publications

(10 citation statements)

References 91 publications

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“…Low temperature is one of the common abiotic stresses affecting plant growth. Plants adapt to low temperatures by changing their morphology and adjusting the expression of a series of genes involved in complex networks ( Lantzouni et al., 2020 ; Li et al., 2021a ). As an interesting phenotype response to chilling stress, leaves color changes have been concerned in many previous studies ( Ahmed et al., 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis unravels the molecular response of Lonicera japonica leaves to chilling stress

Zhang

et al. 2022

Front. Plant Sci.

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Lonicera japonica is not only an important resource of traditional Chinese medicine, but also has very high horticultural value. Studies have been performed on the physiological responses of L. japonica leaves to chilling, however, the molecular mechanism underlying the low temperature-induced leaves morphological changes remains unclear. In this study, it has been demonstrated that the ratio of pigments content including anthocyanins, chlorophylls, and carotenoids was significantly altered in response to chilling condition, resulting in the color transformation of leaves from green to purple. Transcriptomic analysis showed there were 10,329 differentially expressed genes (DEGs) co-expressed during chilling stress. DEGs were mainly mapped to secondary metabolism, cell wall, and minor carbohydrate. The upregulated genes (UGs) were mainly enriched in protein metabolism, transport, and signaling, while UGs in secondary metabolism were mainly involved in phenylpropaoids-flavonoids pathway (PFP) and carotenoids pathway (CP). Protein-protein interaction analysis illustrated that 21 interacted genes including CAX3, NHX2, ACA8, and ACA9 were enriched in calcium transport/potassium ion transport. BR biosynthesis pathway related genes and BR insensitive (BRI) were collectively induced by chilling stress. Furthermore, the expression of genes involved in anthocyanins and CPs as well as the content of chlorogenic acid (CGA) and luteoloside were increased in leaves of L. japonica under stress. Taken together, these results indicate that the activation of PFP and CP in leaves of L. japonica under chilling stress, largely attributed to the elevation of calcium homeostasis and stimulation of BR signaling, which then regulated the PFP/CP related transcription factors.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis unravels the molecular response of Lonicera japonica leaves to chilling stress

Zhang

et al. 2022

Front. Plant Sci.

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“…The WRKY transcription factor family plays a role in plant response to abiotic stresses such as cold and drought [ 16 , 38 ]. In this research, WRKY48 was upregulated in grafted cucumbers from both rootstocks, suggesting that WRKY48 expression may be influenced by the rootstock and is positively correlated with cold tolerance in cucumber.…”

Section: Discussionmentioning

confidence: 99%

“…Lipid metabolism and a range of redox processes are reported to play a significant role in the response of crops to cold stress [ 14 ]. Lipid metabolism, including membrane lipid and fatty acid metabolism, highly influences the response to cold stress [ 15 , 16 ]. Phenylpropanoid metabolism [ 17 ], phytohormone signaling [ 18 ], and transcription factors [ 19 ] have also been reported to regulate cold tolerance in crops.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome and Physiological Analysis of Rootstock Types and Silicon Affecting Cold Tolerance of Cucumber Seedlings

Luan

Niu

Nie

et al. 2022

Plants

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Cucumbers grafted on rootstocks with different wax-removing abilities show varying levels of cold tolerance. The content of pericarp wax powder correlates with its silicon-metabolizing capacity, and rootstock grafting can alter not only the cold tolerance but also the silicon-metabolizing capacity of the scion. The molecular mechanisms responsible for resistance due to rootstocks and silicon and the pathway that affects cold tolerance, however, remain poorly understood. Therefore, we performed physiological and transcriptome analysis to clarify how rootstock types and silicon affect cold tolerance in cucumber seedlings. Then, we randomly selected eight differentially expressed genes (DEGs) for quantitative real time PCR (qRT-PCR) analysis to proof the reliability of the transcriptome data. The results showed that silicon can enhance the cold tolerance of cucumbers by boosting the phenylpropanoid metabolism, and rootstock grafting can boost the active oxygen scavenging ability and synthesis level of hormones in cucumbers and maintain the stability of the membrane structure to enhance cold tolerance. The difference in cold tolerance between the two rootstocks is because the cold-tolerant one has stronger metabolic and sharp signal transduction ability and can maintain the stability of photosynthesis, thereby contributing to the stability of the cellular system and enhancing tolerance to cold.

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“…Omics studies in other plants have revealed the involvement of multiple pathways in protecting plant leaves from cold injury. For example, a combined metabolome and transcriptome study in Cucurbita maxima showed that genes related to phytohormone signaling, carbohydrate metabolism, amino acid metabolism, phenylpropanoid pathway are highly expressed in the cold tolerant genotype [ 16 ]. Whereas in Capsicum annum, metabolome analysis revealed differential accumulation of phytohormones, polyamines and osmolytes along with increased expression of genes related to calcium, MAPK, phytohormone and reactive oxygen species (ROS) signaling [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Cold-stress induced metabolomic and transcriptomic changes in leaves of three mango varieties with different cold tolerance

Kong,

Hou,

Liu

et al. 2024

BMC Plant Biol

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Background Mango (Mangifera indica L.) is grown in Hainan, Guangdong, Yunnan, Sichuan, and Fujian provinces and Guanxi autonomous region of China. However, trees growing in these areas suffer severe cold stress during winter, which affects the yield. To this regard, data on global metabolome and transcriptome profiles of leaves are limited. Here, we used combined metabolome and transcriptome analyses of leaves of three mango cultivars with different cold stress tolerance, i.e. Jinhuang (J)—tolerant, Tainung (T) and Guiremang No. 82 (G)—susceptible, after 24 (LF), 48 (MF) and 72 (HF) hours of cold. Results A total of 1,323 metabolites belonging to 12 compound classes were detected. Of these, amino acids and derivatives, nucleotides and derivatives, and lipids accumulated in higher quantities after cold stress exposure in the three cultivars. Notably, Jinhuang leaves showed increasing accumulation trends of flavonoids, terpenoids, lignans and coumarins, and alkaloids with exposure time. Among the phytohormones, jasmonic acid and abscisic acid levels decreased, while N6-isopentenyladenine increased with cold stress time. Transcriptome analysis led to the identification of 22,526 differentially expressed genes. Many genes enriched in photosynthesis, antenna proteins, flavonoid, terpenoid (di- and sesquiterpenoids) and alkaloid biosynthesis pathways were upregulated in Jihuang leaves. Moreover, expression changes related to phytohormones, MAPK (including calcium and H2O2), and the ICE-CBF-COR signalling cascade indicate involvement of these pathways in cold stress responses. Conclusion Cold stress tolerance in mango leaves is associated with regulation of primary and secondary metabolite biosynthesis pathways. Jasmonic acid, abscisic acid, and cytokinins are potential regulators of cold stress responses in mango leaves.

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Integrating transcriptome and metabolome analyses of the response to cold stress in pumpkin (Cucurbita maxima)

Cited by 16 publications

References 91 publications

Transcriptomic analysis unravels the molecular response of Lonicera japonica leaves to chilling stress

Transcriptomic analysis unravels the molecular response of Lonicera japonica leaves to chilling stress

Transcriptome and Physiological Analysis of Rootstock Types and Silicon Affecting Cold Tolerance of Cucumber Seedlings

Cold-stress induced metabolomic and transcriptomic changes in leaves of three mango varieties with different cold tolerance

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