Integrating the Polydopamine Nanosphere/Aptamers Nanoplatform with a DNase-I-Assisted Recycling Amplification Strategy for Simultaneous Detection of MMP-9 and MMP-2 during Renal Interstitial Fibrosis

Yu, Xie‐an; Hu, Yang; Zhang, Ying; Zhang, Ran; Bai, Xin; Gu, Li; Gao, Han; Li, Renshi; Tian, Jiangwei; Yu, Boyang

doi:10.1021/acssensors.0c00058

Cited by 30 publications

(25 citation statements)

References 44 publications

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“…Indeed, Du et al showed MMP-2 and MMP-9 levels increased over time in damaged tubules and lead to renal brosis, which is a typical phenomenon in progressive renal disease [31]. Other studies have shown MMP-2 plays a pathological role in interstitial renal brosis, probably by inducing EMT and macrophage in ltration [32]. In addition, Neal et al showed increased MMP-2, MMP-9, and TIMP-1 activity in the aorta of patients with progressive kidney disease, and increased activity of MMP-2 in the serum [33].…”

Section: Discussionmentioning

confidence: 99%

Serum levels of matrix metalloproteinases MMP-2, MMP-3, MMP-9 and tissue inhibitor of matrix metalloproteinase TIMP-2 in patients with myocarditis.

Kobusiak−Prokopowicz

Kaaz

Marciniak

et al. 2021

Preprint

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Background: Under physiological conditions, the myocardial extracellular matrix (ECM) is maintained by matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). However, certain stimuli cause the upregulation of MMPs, which can lead to pathological remodeling of the ECM. We assessed serum levels of MMPs and TIMP-2 in patients with myocarditis and their relationship(s) to myocardial damage.Methods: In total, 45 patients with myocarditis who underwent cardiac magnetic resonance imaging were included, comprising 11 with concurrent chronic kidney disease (CKD). Blood samples were obtained to assess serum levels of MMP-2, MMP-3, MMP-9, and TIMP-2. Results: Serum MMP-2, MMP-3, and TIMP-2 levels negatively correlated with ejection fraction values in patients with myocarditis, while MMP-3 levels correlated with longitudinal deformation (p<0.05). Serum MMP-2, MMP-3, and TIMP-2 levels also negatively correlated with renal function, as assessed by the estimated glomerular filtration rate (p<0.05). Patients with myocarditis and concurrent CKD had higher levels of MMP-2 and TIMP-2 than those without kidney damage.Conclusions:1. We demonstrated serum MMP-2, MMP-3, and TIMP-2 concentrations were related to left ventricular ejection fraction, and MMP-3 levels correlated with longitudinal deformation, indicating MMPs play an important role in the post-inflammatory remodeling of the myocardium.2. The occurrence of other heart diseases was an important element in modifying the relationship between MMPs and the degree of myocardial damage.3. Chronic kidney damage in patients with myocarditis results in increased MMP activity. A negative correlation between eGFR and MMP-2, MMP-3 and TIMP-2, and a positive correlation between creatinine and MMP-3 levels, underlines the role of fibrosis in myocarditis with concomitant chronic kidney disease.

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Section: Discussionmentioning

confidence: 99%

Serum levels of matrix metalloproteinases MMP-2, MMP-3, MMP-9 and tissue inhibitor of matrix metalloproteinase TIMP-2 in patients with myocarditis.

Kobusiak−Prokopowicz

Kaaz

Marciniak

et al. 2021

Preprint

View full text Add to dashboard Cite

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“…Indeed, Du et al [32] showed MMP-2 and MMP-9 levels increased over time in damaged tubules and lead to renal fibrosis, which is a typical phenomenon in progressive renal disease. Other studies have shown MMP-2 plays a pathological role in interstitial renal fibrosis, probably by inducing epithelial-to-mesenchymal transition and macrophage infiltration [33]. In addition, Chen et al [34]showed increased MMP-2, MMP-9, and TIMP-1 activity in the aorta of patients with progressive kidney disease and increased activity of MMP-2 in the serum.…”

Section: Discussionmentioning

confidence: 99%

Relationships between Circulating Matrix Metalloproteinases, Tissue Inhibitor TIMP-2, and Renal Function in Patients with Myocarditis

Kobusiak−Prokopowicz

Kaaz

Marciniak

et al. 2021

Kidney Blood Press Res

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Introduction: Under physiological conditions, the myocardial extracellular matrix (ECM) is maintained by matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). However, changes in the balance between MMPs and TIMPs can lead to pathological remodeling of the ECM, which contributes to cardiovascular and kidney diseases. The aim of our study was to assess levels of MMPs and TIMP-2 in patients with myocarditis and their relationship to renal function. Materials and Methods: Forty five patients with myocarditis who underwent CMR were included, comprising 11 with concurrent chronic kidney disease (CKD). Blood samples were obtained to assess serum levels of MMP-2, MMP-3, MMP-9, and TIMP-2. Results: Serum MMP-2, MMP-3, and TIMP-2 levels negatively correlated with the ejection fraction in patients with myocarditis, while MMP-3 levels correlated with longitudinal deformation (p < 0.05). Serum MMP-2, MMP-3, and TIMP-2 levels also negatively correlated with renal function, as assessed by the estimated glomerular filtration rate (eGFR) (p < 0.05). Patients with myocarditis and concurrent CKD had higher levels of MMP-2 and TIMP-2 than those without kidney damage. Conclusions: (1) We demonstrated that MMP-2, MMP-3, and TIMP-2 concentrations were related to left-ventricular ejection fraction, and MMP-3 levels correlated with longitudinal deformation, indicating MMPs play an important role in the post-inflammatory remodeling of the myocardium. (2) A negative correlation between the eGFR and MMP-2, MMP-3, and TIMP-2 and a positive correlation between creatinine and MMP-3 levels indicate the role of MMPs and TIMP-2 in renal dysfunction.

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“…Likewise, a strategy based on the nanoplatform composed of the PDA nanosphere and fluorescence‐labeled aptamers was developed to simultaneously detect MMP‐9 and MMP‐2 with DNase‐I‐assisted recycling signal amplification. [ 158 ]…”

Section: Pda Nanostructures With High Surface Areas Toward Adhesion‐driven Bioapplicationsmentioning

confidence: 99%

“…Likewise, a strategy based on the nanoplatform composed of the PDA nanosphere and fluorescence-labeled aptamers was developed to simultaneously detect MMP-9 and MMP-2 with DNase-I-assisted recycling signal amplification. [158] Significantly, one of the most significant development breakthroughs for PDA-based nucleic acid carriers could be the successful transition in the particle-oligonucleotide interaction designs from adsorption on the particles' outer surface to loading into the mesopores. After the controllable construction of new PDA nanostructures with porous structures developed by our team, we studied the porous PDA particles used in miRNA detection and comprehensively understood the pore-oligonucleotide interactions.…”

Section: Adhesion-based Sensing Delivery and Integration Of Biomacromolecules And Polymersmentioning

confidence: 99%

Intervention of Polydopamine Assembly and Adhesion on Nanoscale Interfaces: State‐of‐the‐Art Designs and Biomedical Applications

Xie

Tang

Xing

et al. 2021

Adv Healthcare Materials

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The translation of mussel‐inspired wet adhesion to biomedical engineering fields have catalyzed the emergence of polydopamine (PDA)‐based nanomaterials with privileged features and properties of conducting multiple interfacial interactions. Recent concerns and progress on the understanding of PDA's hierarchical structure and progressive assembly are inspiring approaches toward novel nanostructures with property and function advantages over simple nanoparticle architectures. Major breakthroughs in this field demonstrated the essential role of π–π stacking and π‐cation interactions in the rational intervention of PDA self‐assembly. In this review, the recently emerging concepts in the preparation and application of PDA nanomaterials, including 3D mesostructures, low‐dimensional nanostructures, micelle/nanoemulsion based nanoclusters, as well as other multicomponent nanohybrids by the segregation and organization of PDA building blocks on nanoscale interfaces are outlined. The contribution of π‐electron interactions on the interfacial loading/release of π electron‐rich molecules (nucleic acids, drugs, photosensitizers) and the exogenous coupling of optical energy, as well as the impact of wet‐adhesion interactions on the nano‐bio interface interplay, are highlighted by discussing the structure‐property relationships in their featured applications including fluorescent biosensing, gene therapy, drug delivery, phototherapy, combined therapy, etc. The limitations of current explorations, and future research directions are also discussed.

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Integrating the Polydopamine Nanosphere/Aptamers Nanoplatform with a DNase-I-Assisted Recycling Amplification Strategy for Simultaneous Detection of MMP-9 and MMP-2 during Renal Interstitial Fibrosis

Cited by 30 publications

References 44 publications

Serum levels of matrix metalloproteinases MMP-2, MMP-3, MMP-9 and tissue inhibitor of matrix metalloproteinase TIMP-2 in patients with myocarditis.

Serum levels of matrix metalloproteinases MMP-2, MMP-3, MMP-9 and tissue inhibitor of matrix metalloproteinase TIMP-2 in patients with myocarditis.

Relationships between Circulating Matrix Metalloproteinases, Tissue Inhibitor TIMP-2, and Renal Function in Patients with Myocarditis

Intervention of Polydopamine Assembly and Adhesion on Nanoscale Interfaces: State‐of‐the‐Art Designs and Biomedical Applications

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