Integrating strategies of chemistry, biochemistry and metabolomics for characterization of the medication principle of “treating cold/heat syndrome with hot/cold herbs”

Zhou, Ning; Yang, Yaxiong; Li, Kai; Ke, Yingying; Zheng, Xiaoke; Feng, Weisheng; Bai, Zhiyao; Liu, Tong; Wang, Yangyang; Liu, Zhen; Li, Xingcan

doi:10.1016/j.jep.2019.111899

Cited by 23 publications

(10 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…frontiersin.org 10 temperature decrement (Zhou et al, 2019). In addition, the decreased levels of T3 and T4, increased TSH concentration, as well as a damaged thyroid tissue also confirmed the success of model building (Ahmed et al, 2010).…”

Section: Frontiers In Pharmacologysupporting

confidence: 53%

“…And previous experiments in our laboratory have reported that a typical “hot” herb ( Aconiti lateralis radix praeparaia ) could improve hypothyroidism in rats with cold syndrome by adjusting the substances and energy metabolism ( Xiao et al, 2017 ). In this study, a series of typical clinical features of hypothyroidism was observed in the hypo group, including huddling up together inactively, struggling weakly during catching, less water and food intake, and a rectal temperature decrement ( Zhou et al, 2019 ). In addition, the decreased levels of T3 and T4, increased TSH concentration, as well as a damaged thyroid tissue also confirmed the success of model building ( Ahmed et al, 2010 ).…”

Section: Discussionmentioning

confidence: 88%

“…Tryptophan is an essential amino acid, involved in a variety of metabolisms, such as energy metabolism ( Zhou et al, 2019 ) and thyroid hormone metabolism ( Pereira et al, 2017 ). It is reported that a dietary tryptophan deficiency can alter the thyroid hormone levels ( Vaccari et al, 1990 ) and lightened depression or anorexia and a poor appetite can be caused by low thyroid hormone levels ( Pereira et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Application of metabolomics and network analysis to reveal the ameliorating effect of four typical “hot” property herbs on hypothyroidism rats

et al. 2022

View full text Add to dashboard Cite

Herbs with a “hot” properties are frequently used to treat cold symptoms in TCM. However, the underlying mechanisms of the herbs with “hot” properties on hypothyroidism have not been investigated. This study aimed to explore four typical “hot” and “cold” property herb on hypothyroidism. Firstly, the difference efficacy between the four typical “hot” property herbs and the four typical “cold” property herbs was assessed by physical signs, thyroid function, and the metabolic profile using multivariate statistical analysis. The influence of the four typical “hot” property herbs on hypothyroidism was validated pathologically. The impact mechanism of the four typical “hot” property herbs on hypothyroidism was investigated through a metabolomics method combined with network analysis. Na+/K+-ATP, ACC1 enzyme, UCP-1, and the PI3K-Akt pathway were used to confirm the metabolite pathways and target-associated metabolites. The results showed that the four typical “hot” property herbs could significantly improve physical signs, thyroid function, and the metabolic profile in hypothyroidism rats, the four typical “cold” property herbs did not show any benefit. Moreover, the four typical “hot” property herbs could improve lipid metabolism, energy metabolism, and thyroid hormone levels by the PI3K-Akt signaling pathway, Ca2+- AMPK signaling pathways, purine metabolism, and tryptophan metabolism. Additionally, the levels of UCP-1, Na+/K + -ATP enzyme, and ACC1 were ameliorated by the four typical “hot” property herbs in hypothyroidism rats. Therefore, a metabolomics strategy combined with network analysis was successfully performed and interpreted the mechanism of the four typical “hot” property herbs on hypothyroidism based on the theory of “cold and hot” properties of TCM well.

show abstract

Section: Frontiers In Pharmacologysupporting

confidence: 53%

Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Application of metabolomics and network analysis to reveal the ameliorating effect of four typical “hot” property herbs on hypothyroidism rats

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Metabolomics provide an opportunity to develop the systematic analysis of metabolites and have been successfully applied to establishing biomarkers and perturbed pathways which, in turn, can clarify the mechanism of action of traditional Chinese medicine. [47][48][49] In this study, results show that biochemical indicators (such as ALT, AST, ALP, LDH, and TBIL) cannot be used as indicators of liver damage caused by PMR and PMRP. A nontargeted metabolic profile based on UPLC/Q-TOF-MS was developed to investigate significant metabolic changes in rat serum between PMR and PMRP groups.…”

Section: Discussionmentioning

confidence: 85%

A Serum Metabolomic Study on Rats Induced by Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata by Pattern Recognition and Pathways Analysis

Zhang

Huang

Gao

2020

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

This study focused on the differential metabolomic effects between water extracts of Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata in rats. The extracts were subsequently administered for 28 d. Serum biochemical indicators were tested, hematoxylin-eosin staining and immunohistochemistry staining were used to detect histopathological changes in the livers. Ultra-performance LC/quadrupole timeof-flight mass spectrometry was used to detect the changes in endogenous metabolites. Finally, we performed detailed analysis of the changes in metabolic pathways. Hematoxylin-eosin staining and immunohistochemistry staining results indicated that the water extracts of Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata had mild liver injury effect. Fifty-two differential endogenous biomarkers were confirmed as potential biomarkers between Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata groups. In the positive ion mode, the biomarkers included 31 Phosphatidyl cholines (PCs), six lysoPCs, and ceramide. In the negative ion mode, 12 biomarkers were confirmed, including glycodeoxycholic acid, chenodeoxycholic acid, and deoxycholic acid, etc. In Hydrophilic Interaction Liquid Chromatography (HILIC) mode, nine biomarkers were confirmed, including niacinamide, L-palmitoylcarnitine, and butyrylcarnitine, etc. Using MetaboAnalyst 4.0, six related metabolic pathways, including taurine and hypotaurine metabolism, sphingolipid metabolism, glycerophospholipid metabolism, nicotinate and nicotinamide metabolism, arginine and proline metabolism, and tryptophan metabolism and primary bile synthesis, were confirmed as the most differential pathways between the Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata groups.

show abstract

“…While the hot property was associated with inflammation and immune regulation, the cold property was associated with cell growth, proliferation, and development. Zhou et al identified the cold- and hot-specific metabolic profiles as well as the pathways of 7 cold- and 7 hot-temperament herbs using biochemical and metabolomic approaches [ 13 ]. The cold property was associated with promoting arginine and proline metabolism, tryptophan metabolism, and ATP storage and generation, while the hot property had the opposite effect.…”

Section: Introductionmentioning

confidence: 99%

Systematic Analysis of the Molecular Mechanisms of Cold and Hot Properties of Herbal Medicines

Park

Baek

Ban

et al. 2022

Plants

View full text Add to dashboard Cite

Effective treatments for patients experiencing temperature-related symptoms are limited. The hot and cold effects of traditional herbal medicines have been utilized to treat and manage these symptoms, but their molecular mechanisms are not fully understood. Previous studies with arbitrarily selected herbs and ingredients may have produced biased results. Here, we aim to systematically elucidate the molecular mechanisms of the hot and cold properties of herbal medicines through an unbiased large-scale investigation of herbal ingredients, their target genes, and the transcriptome signatures induced by them. Using data regarding 243 herbs retrieved from two herbal medicine databases, we statistically identify (R)-Linalool, (-)-alpha-pinene, peruviol, (L)-alpha-terpineol, and cymol as five new hot-specific ingredients that share a common target, a norepinephrine transporter. However, no significant ingredients are cold-specific. We also statistically identify 14 hot- and 8 cold-specific new target genes. Pathway enrichment analysis of hot-specific target genes reveals the associated pathways including neurotransmitter reuptake, cold-induced thermogenesis, blood pressure regulation, adrenergic receptor signaling, and cation symporter activity. Cold-specific target genes are associated with the steroid pathway. Transcriptome analysis also shows that hot herbs are more strongly associated with coagulation and synaptic transmission than cold herbs. Our results, obtained from novel connections between herbal ingredients, target genes, and pathways, may contribute to the development of pharmacological treatment strategies for temperature-related pain using medicinal plants.

show abstract

Integrating strategies of chemistry, biochemistry and metabolomics for characterization of the medication principle of “treating cold/heat syndrome with hot/cold herbs”

Cited by 23 publications

References 22 publications

Application of metabolomics and network analysis to reveal the ameliorating effect of four typical “hot” property herbs on hypothyroidism rats

Application of metabolomics and network analysis to reveal the ameliorating effect of four typical “hot” property herbs on hypothyroidism rats

A Serum Metabolomic Study on Rats Induced by Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata by Pattern Recognition and Pathways Analysis

Systematic Analysis of the Molecular Mechanisms of Cold and Hot Properties of Herbal Medicines

Contact Info

Product

Resources

About