Integrating Shear Flow and Trypsin Treatment to Assess Cell Adhesion Strength
Antra Patel,
Bhavana Bhavanam,
Trevor Keenan
et al.
Abstract:Cell adhesion is of fundamental importance in cell and tissue organization, and for designing cell-laden constructs for tissue engineering. Prior methods to assess cell adhesion strength for strongly adherent cells using hydrodynamic shear flow either involved the use of specialized flow devices to generate high shear stress or used simpler implementations like larger height parallel plate chambers that enable multi-hour cell culture but generate low shear stress and are hence more applicable for weakly adhere… Show more
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