Integrating proteomics and metabolomics to elucidate the molecular network regulating of inosine monophosphate-specific deposition in Jingyuan chicken

Huang, Zengwen; Cai, Zhengyun; Zhang, Juan; Gu, Yaling; Wang, Jing; Yang, Jinzeng; Lv, Gang; Yang, Chaoyun; Zhang, Yi; Ji, Chen; Jiang, Shengwang

doi:10.1016/j.psj.2023.103118

Cited by 3 publications

(2 citation statements)

References 41 publications

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“…In breast muscle, 77 DE metabolites were screened in positive ion mode, of which 36 were up-regulated and 41 down-regulated; 67 DE metabolites were screened in negative ion mode, of which 34 were up-regulated and 33 down-regulated ( Supplementary Figures S1A,B ). The enrichment analysis with the KEGG pathway database ( 23 ) was performed on the identified DE metabolites and the DE metabolites were mainly enriched in pathways, such as Aminoacyl-tRNA biosynthesis, Purine metabolism, Glycine, serine and threonine metabolism and Arginine and profile metabolism ( Figures 1A – C ). In the purine metabolism pathway, there are 6 DE metabolites downregulated, including L-Glutamine, Adenosine monophosphate (AMP), Hypoxanthine, Guanosine, AICAR and Adenylsuccinic acid ( Figure 1E ).…”

Section: Resultsmentioning

confidence: 99%

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Functional identification of PGM1 in the regulating development and depositing of inosine monophosphate specific for myoblasts

Zhao,

Cai,

Wei

et al. 2023

Front. Vet. Sci.

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BackgroundInosine monophosphate (IMP) is naturally present in poultry muscle and plays a key role in improving meat flavour. However, IMP deposition is regulated by numerous genes and complex molecular networks. In order to excavate key candidate genes that may regulate IMP synthesis, we performed proteome and metabolome analyses on the leg muscle, compared to the breast muscle control of 180-day-old Jingyuan chickens (hens), which had different IMP content. The key candidate genes identified by a differential analysis were verified to be associated with regulation of IMP-specific deposition.ResultsThe results showed that the differentially expressed (DE) proteins and metabolites jointly involve 14 metabolic pathways, among which the purine metabolic pathway closely related to IMP synthesis and metabolism is enriched with four DE proteins downregulated (with higher expression in breast muscles than in leg muscles), including adenylate kinase 1 (AK1), adenosine monophosphate deaminase 1 (AMPD1), pyruvate kinase muscle isoenzyme 2 (PKM2) and phosphoglucomutase 1 (PGM1), six DE metabolites, Hypoxanthine, Guanosine, L-Glutamine, AICAR, AMP and Adenylsuccinic acid. Analysis of PGM1 gene showed that the high expression of PGM1 promoted the proliferation and differentiation of myoblasts and inhibited the apoptosis of myoblasts. ELISA tests have shown that PGM1 reduced adenosine triphosphate (ATP) and IMP and uric acid (UA), while enhancing the biosynthesis of hypoxanthine (HX). In addition, up-regulation of PGM1 inhibited the expression of purine metabolism pathway related genes, and promoted the IMP de novo and salvage synthesis pathways.ConclusionThis study preliminarily explored the mechanism of action of PGM1 in regulating the growth and development of myoblasts and specific IMP deposition in Jingyuan chickens, which provided certain theoretical basis for the development and utilization of excellent traits in Jingyuan chickens.

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Section: Resultsmentioning

confidence: 99%

“…The proteome analyses raw data are early data from the laboratory and used the breast muscle as the control group and the leg muscle as the experimental group for proteome analyses analysis ( 23 ). In short, the sample quality meets the analyses requirements.…”

Section: Methodsmentioning

confidence: 99%

Functional identification of PGM1 in the regulating development and depositing of inosine monophosphate specific for myoblasts

Zhao,

Cai,

Wei

et al. 2023

Front. Vet. Sci.

Self Cite

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PKM2 promotes myoblast growth and inosine monophosphate-specific deposition in Jingyuan chicken

Zhao,

Cai,

Zhang

et al. 2024

Research in Veterinary Science

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Transcriptome analysis reveals the role of long noncoding RNAs in specific deposition of inosine monphosphate in Jingyuan chickens

Zhao,

Cai,

Jiang

et al. 2024

Journal of Animal Science

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Inosine monphosphate (IMP) is one of the important indicators for evaluating meat flavor, and long noncoding RNAs (lncRNAs) play an important role in its transcription and post-transcriptional regulation. Currently, there is little information about how lncRNA regulates the specific deposition of IMP in chicken muscle. In this study, we used transcriptome sequencing to analyze the lncRNAs of the breast and leg muscles of the Jingyuan chicken and identified a total of 357 differentially expressed lncRNAs (DELs), of which 158 were up-regulated and 199 were down-regulated. There were 2,203 and 7,377 cis- and trans-regulated target genes of lncRNAs, respectively, and we identified the lncRNA target genes that are involved in NEGF signaling pathway, glycolysis/ glucoseogenesis and biosynthesis of amino acids pathways. Meanwhile, 621 pairs of lncRNA-miRNA-mRNA interaction networks were constructed with target genes involved in purine metabolism, fatty acid metabolism, and biosynthesis of amino acids. Next, three interacting meso-networks gga-miR-1603-LNC_000324-PGM1, gga-miR-1768-LNC_000324-PGM1 and gga-miR-21-LNC_011339- AMPD1 were identified as closely associated with IMP-specific deposition. Both differentially expressed genes (DEGs) PGM1 and AMPD1 were significantly enriched in IMP synthesis and metabolism-related pathways, and participated in the anabolic process of IMP in the form of organic matter synthesis and energy metabolism. This study obtained lncRNAs and target genes affecting IMP-specific deposition in Jingyuan chickens based on transcriptome analysis, which deepened our insight into the role for lncRNAs in chicken meat quality.

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Integrating proteomics and metabolomics to elucidate the molecular network regulating of inosine monophosphate-specific deposition in Jingyuan chicken

Cited by 3 publications

References 41 publications

Functional identification of PGM1 in the regulating development and depositing of inosine monophosphate specific for myoblasts

Functional identification of PGM1 in the regulating development and depositing of inosine monophosphate specific for myoblasts

PKM2 promotes myoblast growth and inosine monophosphate-specific deposition in Jingyuan chicken

Transcriptome analysis reveals the role of long noncoding RNAs in specific deposition of inosine monphosphate in Jingyuan chickens

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