Integrating Pharmacology and Gut Microbiota Analysis to Explore the Mechanism of Citri Reticulatae Pericarpium Against Reserpine-Induced Spleen Deficiency in Rats

Zheng, Yonghua; Zeng, Xuan; Chen, Pan; Chen, Tingting; Peng, Wei; Su, Weiwei

doi:10.3389/fphar.2020.586350

Cited by 35 publications

(23 citation statements)

References 59 publications

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“… 2019 ; Zheng et al. 2020 ), which is consistent with the applicable scope of MLS. Based on intraperitoneal injection of reserpine, we induced spontaneous sweating symptoms by intraperitoneal injection of pilocarpine.…”

Section: Introductionsupporting

confidence: 74%

Antiperspirant effects and mechanism investigation of Mulisan decoction in rats based on plasma metabolomics

Yin

et al. 2022

Pharmaceutical Biology

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Context Mulisan decoction (MLS) is a classic formula of traditional Chinese medicine for treating hyperhidrosis. The mechanism remains unclear. Objective To investigate the antiperspirant effect and underlying mechanisms of MLS. Materials and methods Fifty rats were divided into control, model, and three doses of MLS intervention groups ( n = 10). Rats except for control group were induced diseases features of the applicable scope of MLS via i.p. reserpine (0.5 mg/kg/d) for 10 days. From day 11, MLS groups were administrated orally MLS at 0.6, 3, and 15 g/kg once a day for 14 days, respectively. After the last administration, sweating was induced in all rats via s.c. pilocarpine (25 mg/kg), the right hind foot of rats was stained, and sweat point numbers were observed. Rat serum was collected to detect IL-2, IL-6, IFN-γ, and TNF-α. Rat plasma was collected for endogenous metabolite analysis via UPLC-QE-Focus-MS. Results Rats treated with MLS presented a significant decrease in sweat point numbers (13.5%), increase in body weight (13.2%), and promotion in the balance of Th1/Th2 cytokine ratio via increasing IL-2 (38.3%), IFN-γ (20.1%), and TNF-α (22.0%) and decreasing IL-6 (24.7%) compared with the model group ( p < 0.05). Plasma metabolomics disclosed 15 potential biomarkers related to model rats, of which two could be significantly reversed by MLS ( p < 0.05). The involved pathways were pantothenate and CoA biosynthesis, and porphyrin metabolism. Conclusions MLS demonstrated a good antiperspirant effect and metabolism improvement. These findings inspire more clinical study validation on immune improvement and antiperspirant effect.

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Section: Introductionsupporting

confidence: 74%

Antiperspirant effects and mechanism investigation of Mulisan decoction in rats based on plasma metabolomics

Yin

et al. 2022

Pharmaceutical Biology

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“…For example, GM imbalance and subsequent metabolic changes in patients with qi deficiency affected immunity and energy metabolism, thereby increasing the susceptibility to diseases ( Ma et al., 2018 ). GM in patients with spleen deficiency syndrome ( Peng et al., 2020 ), spleen-yang-deficiency syndrome ( Lin et al., 2018 ), and kidney-yang deficiency syndrome ( Chen et al., 2019 ) had changed, while TCM can help to restore the richness and diversity of GM in specific TCM syndromes ( Zheng et al., 2020 ), which provided insights into the correlation between TCM syndromes and GM imbalance and a new method for further studying the pathogenesis of GM regulating specific TCM syndrome. Two studies have shown that GM can help to distinguish the two TCM syndromes of UC ( Zhang et al., 2019 ) and colorectal cancer ( Wang et al., 2020 ), which can be used as the biological basis for TCM syndrome differentiation and treatment of related diseases.…”

Section: Discussionmentioning

confidence: 99%

The interactions between traditional Chinese medicine and gut microbiota: Global research status and trends

Yang

Hao

Wang

et al. 2022

Front. Cell. Infect. Microbiol.

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BackgroundThere is a crosstalk between traditional Chinese medicine (TCM) and gut microbiota (GM), many articles have studied and discussed the relationship between the two. The purpose of this study is to use bibliometric analysis to explore the research status and development trends of the TCM/GM research, identify and analyze the highly cited papers relating to the TCM/GM.MethodsA literature search regarding TCM/GM publications from 2004 to 2021 was undertaken on August 13, 2022. The main information (full record and cited references) of publications was extracted from the Science Citation Index Expanded (SCI-E) of Web of Science Core Collection (WoSCC). The Bibliometrix of R package, CiteSpace and VOSviewer were used for bibliometric analysis.ResultsA total of 830 papers were included. The publication years of papers were from 2004 to 2021. The number of papers had increased rapidly since 2018. China had the most publications and made most contributions to this field. Nanjing University of Chinese Medicine and Beijing University of Chinese Medicine were in the leading productive position in TCM/GM research, Chinese Academy of Chinese Medical Sciences had the highest total citations (TC). Duan Jin-ao from Nanjing University of Chinese Medicine had the largest number of publications, and Tong Xiao-lin from China Academy of Chinese Medical Sciences had the most TC. The Journal of Ethnopharmacology had the most published papers and the most TC. The main themes in TCM/GM included the role of GM in TCM treatment of glucolipid metabolism diseases and lower gastrointestinal diseases; the mechanism of interactions between GM and TCM to treat diseases; the links between TCM/GM and metabolism; and the relationship between GM and oral bioavailability of TCM.ConclusionThis study gained insight into the research status, hotspots and trends of global TCM/GM research, identified the most cited articles in TCM/GM and analyzed their characteristics, which may inform clinical researchers and practitioners’ future directions.

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“…It exerts potential inhibitory activity on SARS-CoV2 Mpro [ 60 ]. Indeed, multiple chemical compounds in ARND targeted on either ACE2 or 3CLpro, such as kaempferol, luteolin and quercetin from Lonicerae Japonicae Flos (drug 3) [ 61 ], baicalein, wogonin and oroxylin A from Scutellariae Radix (drug 4) [ 62 , 63 ] and naringenin from Citri Reticuulatae Pericarpium (drug 8) [ 64 ]. They manifested anti-inflammatory, immunomodulatory, and free radical scavenging activities through their actions on a multitude of molecules encompassing (Caspase 3) CASP3, IL-6, and mitogen-activated protein kinase (MAPK) 1, 8, and 14, in the signaling pathways of IL-17, NF-κB, TNF and NOS [ 63 , 65 , 66 ] to achieve their anti-COVID-19 mechanisms.…”

Section: Resultsmentioning

confidence: 99%

An anti-inflammatory and anti-fibrotic proprietary Chinese medicine nasal spray designated as Allergic Rhinitis Nose Drops (ARND) with potential to prevent SARS-CoV-2 coronavirus infection by targeting RBD (Delta)- angiotensin converting enzyme 2 (ACE2) binding

Yip

Lee

et al. 2022

Chin Med

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Background Since the outbreak of COVID-19 has resulted in over 313,000,000 confirmed cases of infection and over 5,500,000 deaths, substantial research work has been conducted to discover agents/ vaccines against COVID-19. Undesired adverse effects were observed in clinical practice and common vaccines do not protect the nasal tissue. An increasing volume of direct evidence based on clinical studies of traditional Chinese medicines (TCM) in the treatment of COVID-19 has been reported. However, the safe anti-inflammatory and anti-fibrotic proprietary Chinese medicines nasal spray, designated as Allergic Rhinitis Nose Drops (ARND), and its potential of re-purposing for suppressing viral infection via SARS-CoV-2 RBD (Delta)- angiotensin converting enzyme 2 (ACE2) binding have not been elucidated. Purpose To characterize ARND as a potential SARS-CoV-2 entry inhibitor for its possible preventive application in anti-virus hygienic agent. Methods Network pharmacology analysis of ARND was adopted to asacertain gene targets which were commonly affected by COVID-19. The inhibitory effect of ARND on viral infection was determined by an in vitro pseudovirus assay. Furthermore, ARND was confirmed to have a strong binding affinity with ACE2 and SARS-CoV-2 spike-RBD (Delta) by ELISA. Finally, inflammatory and fibrotic cell models were used in conjunction in this study. Results The results suggested ARND not only inhibited pseudovirus infection and undermined the binding affinity between ACE2 and the Spike protein (Delta), but also attenuated the inflammatory response upon infection and may lead to a better prognosis with a lower risk of pulmonary fibrosis. The data in this study also provide a basis for further development of ARND as an antiviral hygienic product and further investigations on ARND in the live virus, in vivo and COVID-19 patients. ARND holds promise for use in the current COVID-19 outbreak as well as in future pandemics. Conclusion ARND could be considered as a safe anti-SARS-CoV-2 agent with potential to prevent SARS-CoV-2 coronavirus infection. Graphical abstract

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Integrating Pharmacology and Gut Microbiota Analysis to Explore the Mechanism of Citri Reticulatae Pericarpium Against Reserpine-Induced Spleen Deficiency in Rats

Cited by 35 publications

References 59 publications

Antiperspirant effects and mechanism investigation of Mulisan decoction in rats based on plasma metabolomics

Antiperspirant effects and mechanism investigation of Mulisan decoction in rats based on plasma metabolomics

The interactions between traditional Chinese medicine and gut microbiota: Global research status and trends

An anti-inflammatory and anti-fibrotic proprietary Chinese medicine nasal spray designated as Allergic Rhinitis Nose Drops (ARND) with potential to prevent SARS-CoV-2 coronavirus infection by targeting RBD (Delta)- angiotensin converting enzyme 2 (ACE2) binding

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