Integrating optical tweezers with up-converting luminescence: a non-amplification analytical platform for quantitative detection of microRNA-21 sequences

Li, Chengyu; Cao, Di; Song, Chong-Yang; Xu, Chun-Miao; Ma, Xuyan; Zhang, Zhiling; Pang, Dai‐Wen; Tang, Hong‐Wu

doi:10.1039/c7cc01133d

Cited by 20 publications

(13 citation statements)

References 27 publications

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“…After the targets were enriched and the mentioned quantitative analysis was undertaken, it was determined that each case exhibits different amounts of miRNA-21 sequences at the single cell level (Figure B), i.e., 1878, 603, and 162 copies/cell for MCF-7, HepG2, and Jurkat T, respectively. The conspicuous difference is thought to be due to the vital relevance between miRNA-21 and breast cancer . Meanwhile, the quantified data are evidenced to agree with the real-time PCR (RT-PCR) analysis (Figure S16 and Figure C), demonstrating the reliability of the proposed analytical technique.…”

Section: Resultsmentioning

confidence: 52%

“…Based on the above evidence, we were inspired to integrate OT with specially designed luminescence confined UCNPs to pave a new way to a significantly enhancing bead-supported detection method. The assay principle is illustrated in Scheme , where a kind of next-generation biomarker (miRNA-21 sequences) , for breast cancer is selected as the target model. Concretely, the innovative sandwich structured UCNPs (SUCNPs) with blue emission at around 480 nm are first fabricated to wholly restrict the lanthanide activators (Tm 3+ ) into the inner shell with an extremely narrow space range (≈ 3 nm).…”

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confidence: 99%

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Breaking Through Bead-Supported Assay: Integration of Optical Tweezers Assisted Fluorescence Imaging and Luminescence Confined Upconversion Nanoparticles Triggered Luminescent Resonance Energy Transfer (LRET)

Kang

et al. 2019

Anal. Chem.

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Herein, a conceptual approach for significantly enhancing a bead-supported assay is proposed. For the fluorescence imaging technology, optical tweezers are introduced to overcome the fluid viscosity interference and immobilize a single tested bead at the laser focus to guarantee a fairly precise imaging condition. For the selection of fluorescent materials and the signal acquisition means, a type of innovative luminescence confined upconversion nanoparticle with a unique sandwich structure is specially designed to act as an efficient energy donor to trigger the luminescent resonance energy transfer (LRET) process. By further combining the double breakthrough with a molecular beacon model, the newly developed detection strategy allows for achieving a pretty high LRET ratio (≈ 88%) to FAM molecules and offering sound assay performance toward miRNA analysis with a detection limit as low as the sub-fM level, and is capable of well identifying single-base mismatching. Besides, this approach not only is able to accurately qualify the low-abundance targets from as few as 30 cancer cells but also can be employed as a valid cancer early warning tool for performing liquid biopsy.

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Section: Resultsmentioning

confidence: 52%

mentioning

confidence: 99%

Breaking Through Bead-Supported Assay: Integration of Optical Tweezers Assisted Fluorescence Imaging and Luminescence Confined Upconversion Nanoparticles Triggered Luminescent Resonance Energy Transfer (LRET)

Kang

et al. 2019

Anal. Chem.

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“…Logically, two key points should be considered before putting the proposed suspension array into effect. Unlike our previous work by using a single NIR beam to trap 3 μm beads, , in this study we attempt to split the same laser beam to generate an array of 3 × 3 traps. Thus, it is critical to calibrate whether this case is robust enough to implement multiple trapping.…”

Section: Resultsmentioning

confidence: 99%

Combining Holographic Optical Tweezers with Upconversion Luminescence Encoding: Imaging-Based Stable Suspension Array for Sensitive Responding of Dual Cancer Biomarkers

Cao

et al. 2018

Anal. Chem.

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Establishment of a stable analytical methodology with high-quality results is an urgent need for screening cancer biomarkers in early diagnosis of cancer. In this study, we incorporate holographic optical tweezers with upconversion luminescence encoding to design an imageable suspension array and apply it to conduct the detection of two liver cancer related biomarkers, carcinoembryonic antigen and alpha fetal protein. This bead-based assay is actualized by forming a bead array with holographic optical tweezers and synchronously exciting the upconversion luminescence of corresponding trapped complex beads fabricated with a simple one-step sandwich immunological recognition. Owing to the fact that these flowing beads are stably trapped in the focal plane of the objective lens which tightly converges the array of the laser beams by splitting a 980 nm beam using a diffraction optical element, a fairly stable excitation condition is achieved to provide reliable assay results. By further taking advantage of the eminent encoding capability of upconversion nanoparticles and the extremely low background signals of anti-Stokes luminescence, the two targets are well-identified and simultaneously detected with quite sound sensitivity and specificity. Moreover, the potential on-demand clinical application is presented by employing this approach to respond the targets toward complex matrices such as serum and tissue samples, offering a new alternative for cancer diagnosis technology.

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“…Liang et al combined optical tweezers and “light knife” to perform surgery on cells and organelles, including suspension, movement, sorting, and fusion, etc., and made quantitative measurement of the internal mechanical properties of cells and the interaction between cells [ 6 ]. Li and Tang et al successfully reported the sorting [ 7 ] and sequencing [ 8 ] of a single chromosome. The rigidity caused by molecular shrinkage has also been accurately characterized by optical tweezers [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Numerical and Experimental Investigation on the Optical Manipulation from an Axicon Lensed Fiber

et al. 2021

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Here we numerically and experimentally studied the optical trapping on a microsphere from an axicon lensed fiber (ALF). The optical force from the fiber with different tapered lengths and by incident light at different wavelengths is calculated. Numerically, the microsphere can be trapped by the fiber with tapered outline and at a short incident wavelength of 900 nm. While for the fiber with tapered outline , the microsphere can be trapped by the light with longer wavelength of 1100 nm, 1300 nm, or 1500 nm. The optical trapping to a polystyrene microsphere is experimentally demonstrated in a microfluidic channel and the corresponding optical force is derived according to the fluid flow speed. This study can provide a guidance for future tapered fibre design for optical trapping to microspheres.

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Integrating optical tweezers with up-converting luminescence: a non-amplification analytical platform for quantitative detection of microRNA-21 sequences

Abstract: We report a single-microsphere based imaging assay method by integrating up-converting luminescence with optical tweezers for detecting microRNA-21 sequences. This method achieves a competitive detection limit of 12 fM with good selectivity and no dedicated signal amplification designs.

Cited by 20 publications

References 27 publications

Breaking Through Bead-Supported Assay: Integration of Optical Tweezers Assisted Fluorescence Imaging and Luminescence Confined Upconversion Nanoparticles Triggered Luminescent Resonance Energy Transfer (LRET)

Breaking Through Bead-Supported Assay: Integration of Optical Tweezers Assisted Fluorescence Imaging and Luminescence Confined Upconversion Nanoparticles Triggered Luminescent Resonance Energy Transfer (LRET)

Combining Holographic Optical Tweezers with Upconversion Luminescence Encoding: Imaging-Based Stable Suspension Array for Sensitive Responding of Dual Cancer Biomarkers

Numerical and Experimental Investigation on the Optical Manipulation from an Axicon Lensed Fiber

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