Integrating omics and network pharmacology reveals the anti-constipation role of chitosan with different molecular weights in constipated mice

Liang, Yuxuan; Wei, Xiaoyi; Deng, Jie; Cheng, Peng; Ren, Rui; Luo, Yanying; Zhang, Jiexin; Wei, Xiaoqun; Hardiman, Gary; Sun, Yong; Wang, Hong

doi:10.1016/j.ijbiomac.2023.123930

Cited by 7 publications

(3 citation statements)

References 38 publications

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“…43 Additionally, the metabolomics findings demonstrated that XOS treatment of constipation was closely related to its regulation of alpha-linolenic acid metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, and unsaturated fatty acid biosynthesis, all of which have been shown to improve constipation relief significantly. 44–46 These findings also indicated that several metabolic pathways were noticeably reversed in mice receiving XOS supplementation, highlighting the critical function of XOSs in the anti-constipation effect.…”

Section: Discussionmentioning

confidence: 75%

Integrated metabolomics and transcriptomics revealed the anti-constipation mechanisms of xylooligosaccharides from corn cobs

Song,

Guo,

Sun

et al. 2024

Food Funct.

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Section: Discussionmentioning

confidence: 75%

Integrated metabolomics and transcriptomics revealed the anti-constipation mechanisms of xylooligosaccharides from corn cobs

Song,

Guo,

Sun

et al. 2024

Food Funct.

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“…They also reported that chitosan oligosaccharides failed to improve these constipated mice with gut microbiota depletion. Liang et al [51] reported that low-Mw chitosan (1 kDa) showed better laxative effects on constipated mice than high-Mw chitosan in terms of GI transit and defecation frequency. It also significantly upregulated the levels of Bacteroides and Parabacteroides, while it downregulated the level of Desulfovibrio, as well as promoted the production of SCFAs.…”

Section: Discussionmentioning

confidence: 99%

Gastrointestinal Fermentable Polysaccharide Is Beneficial in Alleviating Loperamide-Induced Constipation in Mice

Liu,

Zhang,

Liu

et al. 2023

Nutrients

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To investigate the role of gastrointestinal (GI) polysaccharide fermentation in alleviating constipation, two polysaccharide fractions were isolated from a soluble fiber extract with determined anti-constipation activity: a 2.04 kDa neutral fraction (SSP-1) contained 99.29% glucose, and a 41.66 kDa acidic fraction (SSP-2) contained 63.85% uronic acid. After mice were given loperamide for 14 d to induce constipation, the GI transit rate increased significantly in the SSP-1 group (p < 0.05) but not in the SSP-2 group. The stool weight in the SSP-2 group was significantly higher than that in SSP-1 (383.60 mg vs. 226.23 mg) (p < 0.05). Both SSP-1 and SSP-2 groups had significantly increased serum gastrin and motilin levels (p < 0.05) and changes in their fecal short-chain fatty acid (SCFA) profiles, while SSP-1 showed better fermentation properties than SSP-2 in terms of statistically higher fecal contents of acetic acid and total SCFAs (p < 0.05). Bioinformatic analysis indicated that SSP-1 upregulated bacteria such as Oscillibacter to improve SCFA metabolism and stimulate GI hormone secretion, while SSP-2 had less influence on the gut microbiota. These results suggest that the neutral polysaccharide with superior GI fermentation properties exerted beneficial effects on constipation, while the less fermentable pectic fraction might act as a stool-bulking agent.

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“…[ 29 ] For protein-protein interaction (PPI) network analysis, enter the intersection gene set into the string database ( http://string-db.org/ ), limit the species to “ Homo sapiens ,” set the confidence value to > 0.4, then use Cytoscape software (version 3.9.1) to optimize the PPI network diagram. [ 30 , 31 ] Additionally, the top 10 nodes ranked by degree score were chosen as hub genes using the CytoHubba algorithm plug-in of the Cytoscape software. [ 32 , 33 ] Lastly, Cytoscape software (version 3.9.1) was used to create the “THSWD-drug-active compound-intersection gene-hub gene-AVNFH” network diagram to illustrate the disease-drug interaction.…”

Section: Methodsmentioning

confidence: 99%

Exploring the potential mechanism of Taohong Siwu decoction in the treatment of avascular necrosis of the femoral head based on network pharmacology and molecular docking

Chen,

Song,

Qiu

et al. 2023

Medicine

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Based on network pharmacology and molecular docking, this study seeks to investigate the mechanism of Taohong Siwu decoction (THSWD) in the treatment of avascular necrosis of the femoral head (AVNFH). The Traditional Chinese Medicine Systems Pharmacology database was used in this investigation to obtain the active ingredients and related targets for each pharmaceutical constituent in THSWD. To find disease-related targets, the terms “avascular necrosis of the femoral head,” “necrosis of the femoral head,” “steroid-induced necrosis of the femoral head,” “osteonecrosis,” and “avascular necrosis of the bone” were searched in the databases DisGeNET, GeneCards, Comparative Toxicogenomics Database, and MalaCards. Following the identification of the overlap targets of THSWD and AVNFH, enrichment analysis using gene ontology, Kyoto Encyclopedia of Genes and Genomes, Reactome, and WikiPathways was conducted. The “THSWD-drug-active compound-intersection gene-hub gene-AVNFH” network and protein-protein interaction network were built using Cytoscape 3.9.1 and string, and CytoHubba was used to screen hub genes. The binding activities of hub gene targets and key components were confirmed by molecular docking. 152 prospective therapeutic gene targets were found in the bioinformatics study of ONFH treated with THSWD, including 38 major gene targets and 10 hub gene targets. The enrichment analysis of 38 key therapeutic targets showed that the biological process of gene ontology analysis mainly involved cytokine-mediated signaling pathway, angiogenesis, cellular response to reactive oxygen species, death-inducing signaling complex. The Kyoto Encyclopedia of Genes and Genomes signaling pathway mainly involves TNF signaling pathway, IL-17 signaling pathway, and the Recactome pathway mainly involves Signaling by Interleukins, Apoptosis, and Intrinsic Pathway for Apoptosis. WikiPathways signaling pathway mainly involves TNF-related weak inducer of apoptosis signaling pathway, IL-18 signaling pathway. According to the findings of enrichment analysis, THSWD cured AVNFH by regulating angiogenesis, cellular hypoxia, inflammation, senescence, apoptosis, cytokines, and cellular proliferation through the aforementioned targets and signaling pathways. The primary component of THSWD exhibits a strong binding force with the key protein of AVNFH. This study sheds new light on the biological mechanism of THSWD in treating AVNFH by revealing the multi-component, multi-target, and multi-pathway features and molecular docking mechanism of THSWD.

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Integrating omics and network pharmacology reveals the anti-constipation role of chitosan with different molecular weights in constipated mice

Cited by 7 publications

References 38 publications

Integrated metabolomics and transcriptomics revealed the anti-constipation mechanisms of xylooligosaccharides from corn cobs

Integrated metabolomics and transcriptomics revealed the anti-constipation mechanisms of xylooligosaccharides from corn cobs

Gastrointestinal Fermentable Polysaccharide Is Beneficial in Alleviating Loperamide-Induced Constipation in Mice

Exploring the potential mechanism of Taohong Siwu decoction in the treatment of avascular necrosis of the femoral head based on network pharmacology and molecular docking

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