Integrating nitric oxide, nitrite and hydrogen sulfide signaling in the physiological adaptations to hypoxia: A comparative approach

Fago, Angela; Frank, Jason; Tota, Bruno; Feelisch, Martin; Olson, Kenneth R.; Helbo, Signe; Lefevre, Sjannie; Mancardi, Daniele; Palumbo, Anna; Sandvik, Guro K.; Skovgaard, Nini

doi:10.1016/j.cbpa.2012.01.011

Cited by 39 publications

(27 citation statements)

References 50 publications

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“…9). Finally, nitric oxide is associated with short-term regulation of contractility and excitation-contraction coupling (3,43) [also see a review by Fago et al (9)], and elevated levels of nitrites and nitric oxide have been recorded in the hearts of Crucian carp exposed to anoxia, where they are thought to play a cardioprotective role (42). Thus, a precedent exists for oxygen sensing in the fish myocardium and for this mechanism to potentially regulate muscle performance directly.…”

Section: Effects Of Reduced Pomentioning

confidence: 93%

“…H 2 S is an established oxygen-sensing molecule that accumulates in trout ventricular myocardium when oxygen levels are low (57), has negative inotropic effects on the rat myocardium (20,58), and has several other direct effects on the myocardium, including cardioprotective effects against ischemia (reviewed in Ref. 9). Finally, nitric oxide is associated with short-term regulation of contractility and excitation-contraction coupling (3,43) [also see a review by Fago et al (9)], and elevated levels of nitrites and nitric oxide have been recorded in the hearts of Crucian carp exposed to anoxia, where they are thought to play a cardioprotective role (42).…”

Section: Effects Of Reduced Pomentioning

confidence: 99%

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Increased ventricular stiffness and decreased cardiac function in Atlantic cod (Gadus morhua) at high temperatures

Syme

Gamperl

Nash

et al. 2013

American Journal of Physiology-Regulatory, Integrative and Comp

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We employed the work loop method to study the ability of ventricular and atrial trabeculae from Atlantic cod to sustain power production during repeated contractions at acclimation temperatures (10°C) and when acutely warmed (20°C). Oxygen tension (Po2) was lowered from 450 to 34% air saturation to augment the thermal stress. Preparations worked under conditions simulating either a large stroke volume (35 contractions/min rate, 8-12% muscle strain) or a high heart rate (70 contractions/min, 2-4% strain), with power initially equal under both conditions. The effect of declining Po2 on power was similar under both conditions but was temperature and tissue dependent. In ventricular trabeculae at 10°C (and atria at 20°C), shortening power declined across the full range of Po2 studied, whereas the power required to lengthen the muscle was unaffected. Conversely, in ventricular trabeculae at 20°C, there was no decline in shortening power but an increase in lengthening power when Po2 fell below 100% air saturation. Finally, when ventricular trabeculae were paced at rates of up to 115 contractions/min at 20°C (vs. the maximum of 70 contractions/min in vivo), they showed marked increases in both shortening and lengthening power. Our results suggest that although elevated heart rates may not impair ventricular power as they commonly do isometric force, limited atrial power and the increased work required to expand the ventricle during diastole may compromise ventricular filling and hence, stroke volume in Atlantic cod at warm temperatures. Neither large strains nor high contraction rates convey an apparent advantage in circumventing this.

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Section: Effects Of Reduced Pomentioning

confidence: 93%

Section: Effects Of Reduced Pomentioning

confidence: 99%

Increased ventricular stiffness and decreased cardiac function in Atlantic cod (Gadus morhua) at high temperatures

Syme

Gamperl

Nash

et al. 2013

American Journal of Physiology-Regulatory, Integrative and Comp

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“…in neonates) is unknown. Although there is an increasing awareness of the physiological significance of gasotransmitters in adult lower vertebrates (Fago et al, 2012;Olson et al, 2012), as in mammals, there are few data concerning their role during early development. To our knowledge, only two studies (Hedrick et al, 2005;Porteus et al, 2014) have systematically compared the interactive effects of gasotransmitters and developmental age and only one of these assessed the O 2 chemosensory response (Porteus et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

A role for nitric oxide in the control of breathing in zebrafish (Danio rerio)

Porteus

Pollack

Tzaneva

et al. 2015

Journal of Experimental Biology

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Nitric oxide (NO) is a gaseous neurotransmitter, which, in adult mammals, modulates the acute hypoxic ventilatory response; its role in the control of breathing in fish during development is unknown. We addressed the interactive effects of developmental age and NO in the control of piscine breathing by measuring the ventilatory response of zebrafish (Danio rerio) adults and larvae to NO donors and by inhibiting endogenous production of NO. In adults, sodium nitroprusside (SNP), a NO donor, inhibited ventilation; the extent of the ventilatory inhibition was related to the pre-existing ventilatory drive, with the greatest inhibition exhibited during exposure to hypoxia (P O2 =5.6 kPa). Inhibition of endogenous NO production using L-NAME suppressed the hypoventilatory response to hyperoxia, supporting an inhibitory role of NO in adult zebrafish. Neuroepithelial cells (NECs), the putative oxygen chemoreceptors of fish, contain neuronal nitric oxide synthase (nNOS). In zebrafish larvae at 4 days post-fertilization, SNP increased ventilation in a concentrationdependent manner. Inhibition of NOS activity with L-NAME or knockdown of nNOS inhibited the hypoxic (P O2 =3.5 kPa) ventilatory response. Immunohistochemistry revealed the presence of nNOS in the NECs of larvae. Taken together, these data suggest that NO plays an inhibitory role in the control of ventilation in adult zebrafish, but an excitatory role in larvae.

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“…Also it would be interesting to delineate the possible interaction between H 2 S and NO in the in vivo model of cardioprotection, through either direct chemical interaction 47 or indirectly via modulation of NO-or H 2 S-producing enzymes. 48 Finally, our group recently showed that administration of an H 2 S donor (Na 2 S) during I/R in mice prevents the formation and the activation of the cryopyrin inflammasome, a macromolecular complex responsible for sensing tissue injury, amplifying the inflammatory response, and inducing cell death. 49 It remains to be determined if a natural botanical product as BRJ has a similar anti-inflammatory potency to those of the chemical H 2 S donor in the setting of cardiac I/R injury.…”

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confidence: 99%

Beetroot juice reduces infarct size and improves cardiac function following ischemia–reperfusion injury: Possible involvement of endogenous H₂S

Salloum

Sturz

Yin

et al. 2014

Exp Biol Med (Maywood)

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Ingestion of high dietary nitrate in the form of beetroot juice (BRJ) has been shown to exert antihypertensive effects in humans through increasing cyclic guanosine monophosphate (cGMP) levels. Since enhanced cGMP protects against myocardial ischemia-reperfusion (I/R) injury through upregulation of hydrogen sulfide (H 2 S), we tested the hypothesis that BRJ protects against I/R injury via H 2 S. Adult male CD-1 mice received either regular drinking water or those dissolved with BRJ powder (10 g/L, containing $0.7 mM nitrate). Seven days later, the hearts were explanted for molecular analyses. Subsets of mice were subjected to I/R injury by occlusion of the left coronary artery for 30 min and reperfusion for 24 h. A specific inhibitor of H 2 S producing enzymecystathionine-g-lyase (CSE), DL-propargylglycine (PAG, 50 mg/kg) was given i.p. 30 min before ischemia. Myocardial infarct size was significantly reduced in BRJ-fed mice (15.8 AE 3.2%) versus controls (46.5 AE 3.5%, mean AE standard error [SE], n ¼ 6/group, P < .05). PAG completely blocked the infarct-limiting effect of BRJ. Moreover, BRJ significantly preserved ventricular function following I/R. Myocardial levels of H 2 S and its putative protein target -vascular endothelial growth factor receptor 2 (VEGFR2) were significantly increased by BRJ intake, whereas CSE mRNA and protein content did not change. Interestingly, the BRJ-induced cardioprotection was not associated with elevated blood nitrate-nitrite levels following I/R nor induction of cardiac peroxiredoxin 5, a mitochondrial antioxidant enzyme previously linked to nitrate-induced cardioprotection. We conclude that BRJ ingestion protects against post-I/R myocardial infarction and ventricular dysfunction possibly through CSE-mediated endogenous H 2 S generation. BRJ could be a promising natural and inexpensive nutraceutical supplement to reduce cardiac I/R injury in patients.

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Integrating nitric oxide, nitrite and hydrogen sulfide signaling in the physiological adaptations to hypoxia: A comparative approach

Cited by 39 publications

References 50 publications

Increased ventricular stiffness and decreased cardiac function in Atlantic cod (Gadus morhua) at high temperatures

Increased ventricular stiffness and decreased cardiac function in Atlantic cod (Gadus morhua) at high temperatures

A role for nitric oxide in the control of breathing in zebrafish (Danio rerio)

Beetroot juice reduces infarct size and improves cardiac function following ischemia–reperfusion injury: Possible involvement of endogenous H₂S

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Integrating nitric oxide, nitrite and hydrogen sulfide signaling in the physiological adaptations to hypoxia: A comparative approach

Cited by 39 publications

References 50 publications

Increased ventricular stiffness and decreased cardiac function in Atlantic cod (Gadus morhua) at high temperatures

Increased ventricular stiffness and decreased cardiac function in Atlantic cod (Gadus morhua) at high temperatures

A role for nitric oxide in the control of breathing in zebrafish (Danio rerio)

Beetroot juice reduces infarct size and improves cardiac function following ischemia–reperfusion injury: Possible involvement of endogenous H2S

Contact Info

Product

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About

Beetroot juice reduces infarct size and improves cardiac function following ischemia–reperfusion injury: Possible involvement of endogenous H₂S