Integrating New Tests of Sperm Genetic Integrity into Semen Analysis: Breakout Group Discussion

Perreault, Sally D.; Aitken, Robert J.; Baker, H. W. G.; Evenson, Donald P.; Huszár, Gábor; Irvine, D. Stewart; Morris, Ian D.; Morris, Rebecca A.; Robbins, Wendie A.; Sakkas, Denny; Spanò, Marcello; Wyrobek, Andrew J.

doi:10.1007/978-1-4419-9190-4_23

Cited by 107 publications

(64 citation statements)

References 62 publications

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“…Any damage in this process can lead to a negative influence on male fertility (Kramer & Krawetz, 1997). In fact, highly organized and compacted chromatin has a critical impact on the fertilization process (Evenson et al, 1999;Agarwal & Said, 2003;Perreault et al, 2003). Although previous investigations could determine DNA fragmentation level in very few patients (Table 2), in terms of quantity, this is the largest study in fully globozoospermic men, performed to find out any correlation between DNA damage level and CMA3 positive sperm cells with round head.…”

Section: Discussionmentioning

confidence: 99%

Correlation between sperm DNA fragmentation index and CMA3 positive spermatozoa in globozoospermic patients

et al. 2015

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SUMMARYThe absence of the acrosome causes the situation which is called globozoospermia. There are a few studies, mostly as case reports, about correlation between levels of sperm DNA damage in patients with total round-headed spermatozoa. We investigated this correlation as well as CMA3 positive spermatozoa in 20 globozoospermic men (with more than 90% round-headed spermatozoa) attending to Royan Institute. Semen samples divided into three parts to semen analysis, to measure DNA fragmentation index (DFI) using sperm chromatin structure assay (SCSA) and to detect CMA3 + sperm cells by chromomycin A3 staining and fluorescent microscopy. Our results showed that there were significant differences in sperm concentration, total sperm motility, and normal morphology between patients and controls group (p < 0.001). Moreover, the average of DFI and CMA3 positive spermatozoa in patients group significantly increases compared with control group (p < 0.001). A significant correlation between DFI and CMA3 + in total population was also detected in patients group (r = 0.45, p = 0.046). To our knowledge, this is the largest study about correlation between DNA damage levels and CMA3 positive spermatozoa with round head sperm cells in total globozoospermic men. It seems that the increase in DNA damage may be because of defective sperm DNA compaction, as we detected CMA3 positive sperm cells in these patients.

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Section: Discussionmentioning

confidence: 99%

Correlation between sperm DNA fragmentation index and CMA3 positive spermatozoa in globozoospermic patients

et al. 2015

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“…Regarding alkaline and neutral variant of comet assay different workers have varied opinion in deciding which is better. The alkaline comet assay cannot differentiate between single or double strand breaks, endogenous or induced breaks and moreover the alkali labile sites are not considered specific for infertility [106,107]. The double strand breaks are difficult to repair by the oocyte DNA repair mechanism, making their evaluation more critical than single stranded breaks which can be repaired and hence have lower pathogenicity.…”

Section: Research Laboratory Aspects and Relevance Of Dna Integrity Tmentioning

confidence: 99%

Sperm DNA integrity assays: diagnostic and prognostic challenges and implications in management of infertility

Shamsi

Imam

Dada

2011

J Assist Reprod Genet

135

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Sperm is not a simple carrier of paternal genetic information but its role extends clearly beyond fertilization. Integrity of sperm genome is an essential pre-requisite for birth of healthy offspring and evaluation of sperm should entail DNA integrity analysis. DNA integrity analysis is a better diagnostic and prognostic marker of sperm reproductive potential. Conventional semen analysis emphasizes on sperm concentration, viability, motility and morphology and has been proven to be a poor indicator of reproductive potential and pregnancy outcome. To overcome the drawbacks associated with conventional semen analysis more useful fertility tests and molecular biomarkers have been explored. Among the different tests which have evolved for assessing the sperm reproductive potential, tests for sperm DNA quality are most promising. Sperm DNA damage has been closely associated with numerous indicators of reproductive health including fertilization, embryo quality, implantation, spontaneous abortion, congenital malformations and childhood diseases. It therefore has great potential as a prognostic test for both in vitro and in vivo conception. This review presents an updated account of tests that have better diagnostic and prognostic implications in the evaluation of sperm DNA damage. The basic principles, outline of methodology, advantage, disadvantage, clinical significance of each technique and implications of these tests have been discussed. The logistics of each test with respect to available resources and equipment in an andrology laboratory, the feasibility of performing these tests in routine diagnostic workup of infertile men and the opportunities and challenges provided by DNA testing in male fertility determination are also presented.

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“…Moreover, DNAdamaged sperm still has a chance to form pronuclei at fertilization and even probably allow for a subsequent embryo development in the context of assistant reproduction technology (ART) [3,4], which raises the concern that transmission of damaged DNA to the offspring, particularly at levels that exceed DNA repair capacity of the oocyte, could have serious consequences [5,6]. On account of these factors, several techniques have been developed to detect sperm DNA damage, such as the terminal deoxynucleotidyl transferase-mediated nick end-labeling (TUNEL), sperm chromatin structure assay (SCSA) and comet assay [7].…”

Section: Introductionmentioning

confidence: 99%

Correlation of sperm DNA damage with IVF and ICSI outcomes: A systematic review and meta-analysis

Wang

Cai

et al. 2006

J Assist Reprod Genet

163

110

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Our meta-analysis indicates that sperm DNA damage, as assessed by the TUNEL assay, significantly decreases only the chance of IVF clinical pregnancy, but not that of either IVF fertilization or ICSI fertilization or ICSI clinical pregnancy. Besides, our results also reveal that sperm DNA damage, when assessed by the SCSA, has no significant effect on the chance of clinical pregnancy after IVF or ICSI treatment.

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Integrating New Tests of Sperm Genetic Integrity into Semen Analysis: Breakout Group Discussion

Cited by 107 publications

References 62 publications

Correlation between sperm DNA fragmentation index and CMA3 positive spermatozoa in globozoospermic patients

Correlation between sperm DNA fragmentation index and CMA3 positive spermatozoa in globozoospermic patients

Sperm DNA integrity assays: diagnostic and prognostic challenges and implications in management of infertility

Correlation of sperm DNA damage with IVF and ICSI outcomes: A systematic review and meta-analysis

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