Integrating Nanoscale Technologies with Cryogenics: A Step Towards Improved Biopreservation

Güven, Sinan; Demirci, Utkan

doi:10.2217/nnm.12.158

Cited by 13 publications

(17 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…33 With a freezing rate of 1°C/min, a large number of MSCs can be easily frozen in one vial with the required CPA at low concentrations (<1.5 M). 79 Further, there is no direct contact between cells and nonsterile liquid nitrogen during the freezing process, significantly reducing the potential risk of contamination.…”

Section: Slow Freezingmentioning

confidence: 99%

“…Given the prior concerns, the development of a sterile, closed, fully automated and high throughput system that allows MSCs to be vitrified without any direct exposure to liquid nitrogen is required. 33 For future clinical applications, liquid nitrogen and ejection-based droplet generation systems must be sterilized and operated in sterile hoods. Liquid nitrogen can be sterilized by using ultraviolet radiation or sterile polyetrafluoroethylene cartridge filteration.…”

Section: Figmentioning

confidence: 99%

“…Freezing cells encapsulated in nanoliter droplets on highly hydrophobic nano-rough surfaces and the development of nontoxic nanoscale bio-inspired CPAs are the promising advances in cryopreservation. 33 But for now, MSCs can only be routinely cryopreserved with the existing methods.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cryopreservation of Human Mesenchymal Stem Cells for Clinical Applications: Current Methods and Challenges

Yong

Zaman

Xufeng

et al. 2015

Biopreservation and Biobanking

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) hold many advantages over embryonic stem cells (ESCs) and other somatic cells in clinical applications. MSCs are multipotent cells with strong immunosuppressive properties. They can be harvested from various locations in the human body (e.g., bone marrow and adipose tissues). Cryopreservation represents an efficient method for the preservation and pooling of MSCs, to obtain the cell counts required for clinical applications, such as cell-based therapies and regenerative medicine. Upon cryopreservation, it is important to preserve MSCs functional properties including immunomodulatory properties and multilineage differentiation ability. Further, a biosafety evaluation of cryopreserved MSCs is essential prior to their clinical applications. However, the existing cryopreservation methods for MSCs are associated with notable limitations, leading to a need for new or improved methods to be established for a more efficient application of cryopreserved MSCs in stem cell-based therapies. We review the important parameters for cryopreservation of MSCs and the existing cryopreservation methods for MSCs. Further, we also discuss the challenges to be addressed in order to preserve MSCs effectively for clinical applications.

show abstract

Section: Slow Freezingmentioning

confidence: 99%

Section: Figmentioning

confidence: 99%

See 1 more Smart Citation

Cryopreservation of Human Mesenchymal Stem Cells for Clinical Applications: Current Methods and Challenges

Yong

Zaman

Xufeng

et al. 2015

Biopreservation and Biobanking

View full text Add to dashboard Cite

show abstract

“…213,240 Following the cryopreservation time of interest, frozen tissues and cells are thawed, and ideally samples resume to their biological activity. During this process, cryoprotectant agents (CPAs) are employed to avoid cryoinjury of cells by cooling to temperatures at which intracellular ice formation takes place.…”

Section: Applications Of Micro-scale Manipulation Of Cellsmentioning

confidence: 99%

Manipulating biological agents and cells in micro-scale volumes for applications in medicine

et al. 2013

Self Cite

View full text Add to dashboard Cite

Recent technological advances provide new tools to manipulate cells and biological agents in micro/nano-liter volumes. With precise control over small volumes, the cell microenvironment and other biological agents can be bioengineered; interactions between cells and external stimuli can be monitored; and the fundamental mechanisms such as cancer metastasis and stem cell differentiation can be elucidated. Technological advances based on the principles of electrical, magnetic, chemical, optical, acoustic, and mechanical forces lead to novel applications in point-of-care diagnostics, regenerative medicine, in vitro drug testing, cryopreservation, and cell isolation/purification. In this review, we first focus on the underlying mechanisms of emerging examples for cell manipulation in small volumes targeting applications such as tissue engineering. Then, we illustrate how these mechanisms impact the aforementioned biomedical applications, discuss the associated challenges, and provide perspectives for further development.

show abstract

“…Recently, the ability to protect cells (e.g. stem cells and red blood cells) from cryodamage has been reported using bio‐inspired CPAs, such as ectoine (El Assal et al, ; Grein et al, ; Guven & Demirci, ), trehalose (Eroglu et al, ; Zhang, Oldenhof, Sieme, & Wolkers, ), and anti‐freeze proteins (Carpenter & Hansen, ; Mitchell, Cameron, & Gibson, ). For instance, ectoine is a biocompatible solute discovered in the halophilic bacterium living in extreme environments (e.g.…”

Section: Introductionmentioning

confidence: 99%

Bio-inspired solute enables preservation of human oocytes using minimum volume vitrification

Choi

Assal

et al. 2017

J Tissue Eng Regen Med

Self Cite

View full text Add to dashboard Cite

The ability to cryopreserve human oocytes has significant potential for fertility preservation.Current cryopreservation methods still suffer from the use of conventional cryoprotectants, such as dimethyl sulphoxide (DMSO), causing loss of viability and function. Such injuries result from the toxicity and high concentration of cryoprotectants, as well as mechanical damage of cells due to ice crystal formation during the cooling and rewarming processes. Here we report the preservation of human oocytes following vitrification using an innovative bio-inspired cryoprotectant integrated with a minimum volume vitrification approach. The results demonstrate that the recovered human oocytes maintained viability following vitrification and rewarming. Moreover, when this approach was used to vitrify mouse oocytes, the recovered oocytes preserved their viability and function following vitrification and rewarming. This bioinspired approach substitutes DMSO, a well-known toxic cryoprotectant, with ectoine, a nontoxic naturally occurring solute. The bio-inspired vitrification approach has the potential to improve fertility preservation for women undergoing cancer treatment and endangered mammal species. KEYWORDSbio-inspired material, ectoine, human oocyte, low level of cryoprotectants, naturally inspired, vitrification

show abstract

Integrating Nanoscale Technologies with Cryogenics: A Step Towards Improved Biopreservation

Cited by 13 publications

References 24 publications

Cryopreservation of Human Mesenchymal Stem Cells for Clinical Applications: Current Methods and Challenges

Cryopreservation of Human Mesenchymal Stem Cells for Clinical Applications: Current Methods and Challenges

Manipulating biological agents and cells in micro-scale volumes for applications in medicine

Bio-inspired solute enables preservation of human oocytes using minimum volume vitrification

Contact Info

Product

Resources

About