Integrating multi-omics and regular analyses identifies the molecular responses of zebrafish brains to graphene oxide: Perspectives in environmental criteria

Analysis of Nanotoxicity with Integrated Omics and Mechanobiology

“…Even though single omics approaches are more helpful for the in-depth analysis of biological phenotypes than conventional approaches, the integration of several omics approaches can allow a more comprehensive analysis [ 21 , 23 ]. Thus, the concept of integrated omics was introduced by Dr.…”

Animal Models for the Study of Nucleic Acid Immunity: Novel Tools and New Perspectives

“…Alternatively, microfluidic analyser and sorter enable high content screening of zebrafish larvae based on morphological features and fluorescence signals[103]. These platforms can be extended to sorting of selected experimental clusters for transcriptomic experiments[104]. Currently, the bottleneck of these innovative technologies is the limit of Big data storage, treatment and analyses.…”

“…Currently, the bottleneck of these innovative technologies is the limit of Big data storage, treatment and analyses. Integration of multi-parametric values (imaging, transcriptomic, proteomic) appears now essential to build a global view of the nucleic acid immunity at the level of the individual[104].While murine models are not amenable to high-throughput screening(Figure 3), the last few years have seen a massive revolution in the field of 3D tissue culture (or organoids). Such self-organizing, multi-cellular ex vivo 3D cultures are considered as physiologically relevant representations of organs.…”

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“…Previous studies have shown that graphene can disrupt mitochondrial activity both in tumor cells (Lammel et al, 2013;Zhou et al, 2014) and in the zebrafish brain (Sun et al, 2019), both resulting in cytoskeletal abnormalities. Additionally, GO has been reported to trigger Parkinson's disease-like symptoms in zebrafish larvae, associated with the deregulation of metabolism (Ren et al, 2016).…”