Integrating miRNA and mRNA Expression Profiling Uncovers miRNAs Underlying Fat Deposition in Sheep

Zhou, Guangxian; Wang, Xiaolong; Yuan, Chao; Kang, Dan-Ju; Xu, Xin; Zhou, Jack G.; Geng, Rong; Yang, Yuxin; Yang, Zhaoxia; Chen, Yulin

doi:10.1155/2017/1857580

Cited by 36 publications

(29 citation statements)

References 46 publications

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“…STHS have a high reproductive capacity and show polyembryony; they grow fast and could be in oestrum at all seasons ( Kashan et al, 2005 ). TS are raised in the mountainous region of the Qingzang plateau, where the average elevation is 3,500 m. Compared with LFTS and STHS, TS are relatively stronger and their tails are the smallest with less fat accumulation ( Zhu et al, 2016 ; Zhou et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Profiling of mRNA and lncRNA Related to Tail Adipose Tissues of Sheep

Zhang

Jin

et al. 2018

Front. Genet.

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The Lanzhou Fat-Tail sheep (LFTS, long fat-tailed sheep) is an endangered sheep breed in China with a fat tail compared to the traditional local varieties, Small Tail Han sheep (STHS, thin-tailed sheep) with a small tail, and Tibetan sheep (TS, short thin-tailed sheep) with a little tail. However, little is known regarding how tail fat deposition is regulated by long noncoding RNA (lncRNA). To evaluate the lncRNA and mRNA associated with tail fat deposition and development among these breeds, high-throughput RNA sequencing of three individuals each of LFTS, STHS, and TS were performed and analyzed in this study. RNA sequencing data from these three groups revealed 10 differentially expressed genes (DEGs) and 37 differentially expressed lncRNAs between the LFTS and STHS groups, 390 DEGs and 59 differentially expressed lncRNAs between the LFTS and TS groups, and 80 DEGs and 16 differentially expressed lncRNAs between the STHS and TS groups (p-value < 0.05 and fold change ≥ 2), respectively. Gene Ontology and pathway analysis of DEGs and target genes of differentially expressed lncRNAs revealed enrichment in fatty acid metabolism and fatty acid elongation-related pathways that contribute to fat deposition. Subsequently, the expression of 14 DEGs and 6 differentially expressed lncRNAs was validated by quantitative real-time PCR. Finally, two co-expression networks of differentially expressed mRNA and lncRNAs were constructed. The results suggested that some differentially expressed lncRNAs (TCONS_00372767, TCONS_00171926, TCONS_00054953, and TCONS_00373007) may play crucial roles as core lncRNAs in tail fat deposition processes. In summary, the present study extends the sheep tail fat lncRNA database and these differentially expressed mRNA and lncRNAs may provide novel candidate regulators for future genetic and molecular studies on tail fat deposition of sheep.

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Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Profiling of mRNA and lncRNA Related to Tail Adipose Tissues of Sheep

Zhang

Jin

et al. 2018

Front. Genet.

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“…A study has shown that cir-cRNAs can regulate gene expression through a circRNA-miRNA-mRNA pathway [31]. Another pioneering study has demonstrated that miRNAs are differentiallyexpressed between fat-tailed and short-tailed sheep breeds [61]. However, there remains lack of research on circRNA in sheep SF at different developmental stages.…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of circRNAs in the skin during wool follicle development in Aohan fine wool sheep

et al. 2020

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Background: Aohan fine wool sheep (AFWS) is a historically bred fine wool sheep, cultivated in China. The wool has excellent quality and good textile performance. Investigating the molecular mechanisms that regulate wool growth is important to improve wool quality and yield. Circular RNAs (circRNAs) are widely expressed non-coding RNAs that can act as competitive endogenous RNAs (ceRNAs) to bind to miRNAs. Although circRNAs have been studied in many fields, research on their activity in sheep wool follicles is limited. To understand the regulation of circRNAs in the growth of fine wool in sheep, we used RNA-Seq to identify circRNAs in sheep shoulder skin samples at three developmental stages: embryonic day 90 (E90d), embryonic day 120 (E120d), and at birth (Birth). Results: We identified 8753 circRNAs and found that 918 were differentially-expressed. We then analyzed the classification and characteristic of the circRNAs in sheep shoulder skin. Using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), we identified the source genes of circRNAs, which were mainly enriched in cellular component organization, regulation of primary metabolic processes, tight junctions, and the cGMP-PKG and AMPK signaling pathways. In addition, we predicted interactions between 17 circRNAs and eight miRNAs, using miRanda software. Based on the significant pathways, we speculate that circ_0005720, circ_0001754, circ_0008036, circ_0004032, circ_0005174, circ_0005519, and circ_0007826 might play an important role in regulating wool follicle growth in AFWS. Seven circRNAs were randomly selected to validate the RNA-Seq results, using qRT-PCR. Conclusion: Our results provide more information about circRNAs regulation of wool follicle development in AFWS, and establish a solid foundation for future research.

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“…Research on IMF is extensive in farm animals, such as bovine (Harper and Pethick, 2004;Takenouchi et al, 2004;Taniguchi et al, 2008;Du et al, 2010b;Romao et al, 2011;Cardoso et al, 2012;Huang et al, 2012;Hudson et al, 2015;Guan et al, 2017;Ma et al, 2018), porcine (Hausman and Poulos, 2004;Zhang et al, 2014;Chen et al, 2018;Won et al, 2018), sheep (Dodson et al, 1997;Tong et al, 2008;Zhou et al, 2017;Pan et al, 2018) and chicken (Qiu et al, 2017;Wang et al, 2017a;Cui et al, 2018;Fu et al, 2018), and have brought to the development of a wealth of isolation, growth, characterization and differentiation protocols.…”

Section: Adipocytes and Imfmentioning

confidence: 99%

Tissue Engineering for Clean Meat Production

Ben-Arye

Levenberg

2019

Front. Sustain. Food Syst.

186

134

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Increasing public awareness of foodborne illnesses, factory farming, and the ecological footprint of the meat industry, has generated the need for animal-free meat alternatives. In the last decade, scientists have begun to leverage the knowledge and tools accumulated in the fields of stem cells and tissue engineering toward the development of cell-based meat (i.e., clean meat). In tissue engineering, the physical and biochemical features of the native tissue can be mimicked; cells and biomaterials are integrated under suitable culture conditions to form mature tissues. More specifically, in skeletal muscle tissue engineering, a plurality of cell types can be co-cultured on a 3D scaffold to generate muscle fibers, blood vessels and a dense extracellular matrix (ECM). This review focuses on tissue engineering of skeletal muscle and the adjustments needed for clean meat development. We discuss the skeletal muscle structure and composition, and elaborate on cell types from farm animals that have the potential to recapitulate the muscle ECM, blood vessels, muscle fibers and fat deposits. We also review relevant biomaterials, primarily for fabricating scaffolds that can mimic the intramuscular connective tissues, as well as gene expression studies on the biological pathways that influence meat quality.

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Integrating miRNA and mRNA Expression Profiling Uncovers miRNAs Underlying Fat Deposition in Sheep

Cited by 36 publications

References 46 publications

Comparative Transcriptome Profiling of mRNA and lncRNA Related to Tail Adipose Tissues of Sheep

Comparative Transcriptome Profiling of mRNA and lncRNA Related to Tail Adipose Tissues of Sheep

Identification and characterization of circRNAs in the skin during wool follicle development in Aohan fine wool sheep

Tissue Engineering for Clean Meat Production

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