Integrating metagenetics and high-throughput screening for bioprospecting marine thraustochytrids producers of long-chain polyunsaturated fatty acids

Colonia, Brigitte Sthepani Orozco; Pereira, Gilberto Vinícius de Melo; Rodrigues, Felipe Mendonça; Muynarsk, Elisângela de Souza Miranda; Vale, Alexander da Silva; Carvalho, Júlio César de; Soccol, Vanete Thomaz; Penha, Rafaela de Oliveira; Soccol, Carlos Ricardo

doi:10.1016/j.biortech.2021.125176

Cited by 10 publications

(3 citation statements)

References 41 publications

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“…Changing the carbon source in the culture medium was found to have a significant impact on cellular growth and bioflocculant production. Glucose is a common carbon source used by a wide range of microorganisms (Colonia et al 2021). Carbon sources in the culture medium required for bioflocculant production have been well reported to differ for different microorganisms (Gong et al 2008;Cosa et al 2011).…”

Section: Discussionmentioning

confidence: 99%

A Novel Bioflocculant Produced by Cobetia marina MCCC1113: Optimization of Fermentation Conditions by Response Surface Methodology and Evaluation of Flocculation Performance when Harvesting Microalgae

Zeng

Pan

et al. 2022

Polish Journal of Microbiology

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A preliminary study was carried out to optimize the culture medium conditions for producing a novel microbial flocculant from the marine bacterial species Cobetia marina. The optimal glucose, yeast extract, and glutamate contents were 30, 10, and 2 g/l, respectively, while the optimal initial pH of the culture medium was determined to be 8. Following response surface optimization, the maximum bioflocculant production level of 1.36 g/l was achieved, which was 43.40% higher than the original culture medium. Within 5 min, a 20.0% (v/v) dosage of the yielded bioflocculant applied to algal cultures resulted in the highest flocculating efficiency of 93.9% with Spirulina platensis. The bioflocculant from C. marina MCCC1113 may have promising application potential for highly productive microalgae collection, according to the findings of this study.

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Section: Discussionmentioning

confidence: 99%

A Novel Bioflocculant Produced by Cobetia marina MCCC1113: Optimization of Fermentation Conditions by Response Surface Methodology and Evaluation of Flocculation Performance when Harvesting Microalgae

Zeng

Pan

et al. 2022

Polish Journal of Microbiology

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“…Additionally, Colonia, et al [86] investigated marine thraustochytrids which are lipid accumulating protists found in mangroves and coastal seawaters of southern Brazil. Metagenomics was used to identify the existence of microorganisms that accumulate lipid.…”

Section: Genomics and Metagenomicsmentioning

confidence: 99%

Marine microbial bioprospecting: Exploitation of marine biodiversity towards biotechnological applications—a review

et al. 2022

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The increase in the human population causes an increase in the demand for nutritional supplies and energy resources. Thus, the novel, natural, and renewable resources became of great interest. Here comes the optimistic role of bioprospecting as a promising tool to isolate novel and interesting molecules and microorganisms from the marine environment as alternatives to the existing resources. Bioprospecting of marine metabolites and microorganisms with high biotechnological potentials has gained wide interest due to the variability and richness of the marine environment. Indeed, the existence of extreme conditions that increases the adaptability of marine organisms, especially planktons, allow the presence of interesting biological species that are able to produce novel compounds with multiple health benefits and high economical value. This review aims to provide a comprehensive overview of marine microbial bioprospecting as a growing field of interest. It emphasizes functional bioprospecting that facilitates the discovery of interesting metabolites. Marine bioprospecting was also discussed from a legal aspect for the first time, focusing on the shortcomings of international law. We also summarized the challenges facing bioprospecting in the marine environment including economic feasibility issues.

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“…In addition to the diverse PUFA profiles, the domain organizations between some prokaryotic PUFA synthases are also quite different ( Figure 3 ), which provides good candidates for the studies of prokaryotic PUFA synthases. At present, more PUFA-producing eukaryotic microorganisms are being discovered ( Colonia et al, 2021 ; Kalidasan et al, 2021 ; Wang et al, 2021 ; Bai et al, 2022 ), and species with differentiated PUFA profiles are likely to be discovered in the future. Analyzing the genomes of these species and expressing the possible genes in E. coli will be an efficient way to identify novel PUFA synthases.…”

Section: Challenges and Future Perspectivesmentioning

confidence: 99%

Deciphering and engineering the polyunsaturated fatty acid synthase pathway from eukaryotic microorganisms

Guo

Dong

Wang

et al. 2022

Front. Bioeng. Biotechnol.

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Polyunsaturated fatty acids (PUFAs) are important nutrients that play important roles in human health. In eukaryotes, PUFAs can be de novo synthesized through two independent biosynthetic pathways: the desaturase/elongase pathway and the PUFA synthase pathway. Among them, PUFAs synthesized through the PUFA synthase pathway typically have few byproducts and require fewer reduction equivalents. In the past 2 decades, numerous studies have been carried out to identify, analyze and engineer PUFA synthases from eukaryotes. These studies showed both similarities and differences between the eukaryotic PUFA synthase pathways and those well studied in prokaryotes. For example, eukaryotic PUFA synthases contain the same domain types as those in prokaryotic PUFA synthases, but the number and arrangement of several domains are different; the basic functions of same-type domains are similar, but the properties and catalytic activities of these domains are somewhat different. To further utilize the PUFA synthase pathway in microbial cell factories and improve the productivity of PUFAs, many challenges still need to be addressed, such as incompletely elucidated PUFA synthesis mechanisms and the difficult genetic manipulation of eukaryotic hosts. In this review, we provide an updated introduction to the eukaryotic PUFA synthase pathway, summarize the functions of domains and propose the possible mechanisms of the PUFA synthesis process, and then provide future research directions to further elucidate and engineer the eukaryotic PUFA synthase pathway for the maximal benefits of humans.

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Integrating metagenetics and high-throughput screening for bioprospecting marine thraustochytrids producers of long-chain polyunsaturated fatty acids

Cited by 10 publications

References 41 publications

A Novel Bioflocculant Produced by Cobetia marina MCCC1113: Optimization of Fermentation Conditions by Response Surface Methodology and Evaluation of Flocculation Performance when Harvesting Microalgae

A Novel Bioflocculant Produced by Cobetia marina MCCC1113: Optimization of Fermentation Conditions by Response Surface Methodology and Evaluation of Flocculation Performance when Harvesting Microalgae

Marine microbial bioprospecting: Exploitation of marine biodiversity towards biotechnological applications—a review

Deciphering and engineering the polyunsaturated fatty acid synthase pathway from eukaryotic microorganisms

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