Integrating marker-assisted background analysis with foreground selection for pyramiding bacterial blight resistance genes into Basmati rice

Baliyan, Nikita; Malik, Rekha; Rani, Reema; Mehta, Kirti; Vashisth, Urvashi; Dhillon, Santosh; Boora, K. S.

doi:10.1016/j.crvi.2017.11.003

Cited by 25 publications

(13 citation statements)

References 33 publications

(49 reference statements)

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“…Compared with traditional breeding strategies, MAS can significantly improve selection efficiency; however, pyramiding multiple genes still requires many generations (Jiang et al 2012;Baliyan et al 2018). In this study, using multiple breeding strategies (single cross, mutual intercross, and backcross) combined with MAS, we successfully obtained BC 1 F 1-7 lines carrying Pita/pita, Pib/pib, and Pid2/pid2.…”

Section: Discussionmentioning

confidence: 99%

Superior japonica rice variety YJ144 with improved rice blast resistance, yield, and quality achieved using molecular design and multiple breeding strategies

et al. 2021

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Pib, and Pid2, all of which afford broad-spectrum resistance to blast. A collection of 91 japonica rice germplasms with similar ecological characteristics to YF47 were screened, and Wuyunjing 27 (WYJ27) with Pita and Pib alleles and P135 with the Pid2 allele were identified. Furthermore, the corresponding positive Pita, Pib, and Pid2 alleles were transferred into YF47 using single, mutual, and backcrosses, together with molecular marker-assisted selection (MAS) and anther culture technology. These genetic materials, carrying one, two, or three functional alleles, were generated within 3 years, and compared to YF47, they all showed improved resistance to naturally inoculated rice blast. Further improved lines (IL) 1 to 5 (all containing Pita, Pib, and Pid2 alleles) were evaluated for yield performance, and when no fungicide was applied, all lines except IL-4 showed increased traits compared with those of YF47. IL-5, renamed Yanjing 144 (YJ144), showed yield increases in the Liaoning province regional variety comparison test and superior appearance quality compared to YF47. Our work provides a molecular design strategy for pyramiding multiple beneficial genes to rapidly improve rice blast resistance, yield, and quality using multiple breeding strategies.

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Section: Discussionmentioning

confidence: 99%

Superior japonica rice variety YJ144 with improved rice blast resistance, yield, and quality achieved using molecular design and multiple breeding strategies

et al. 2021

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“…Compared with traditional breeding strategies, MAS can signi cantly improve selection e ciency; however, pyramiding multiple genes still requires many generations (Jiang et al 2012;Baliyan et al 2018). In this study, using multiple breeding strategies (single cross, mutual intercross, and backcross) combined with MAS, we successfully obtained BC 1 F 1-7 lines carrying Pita/pita, Pib/pib and Pid2/pid2.…”

Section: Discussionmentioning

confidence: 99%

Superior japonica rice variety YJ144 with improved rice blast resistance, yield, and quality achieved using molecular design and multiple breeding strategies

Mao

Zhu

Ahmad

et al. 2021

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Yanfeng 47 (YF47) is an elite japonica rice variety cultivated in China on nearly 2 million hectares over the past 20 years. However, YF47 is highly susceptible to rice blast (Magnaporthe oryzae), one of the most destructive rice diseases. In this study, we developed novel TPAP (tetra-primer ARMS-PCR) functional markers for the genes Pita, Pib and Pid2, all of which afford broad-spectrum resistance to blast. A collection of 91 japonica rice germplasms with similar ecological characteristics to YF47 was screened, and Wuyunjing 27 (WYJ27) with Pita and Pib alleles, and P135 with the Pid2 allele, were identified. Furthermore, the corresponding positive Pita, Pib and Pid2 alleles were transferred into YF47 using single, mutual and backcrosses, together with molecular marker-assisted selection (MAS) and anther culture technology. These genetic materials, carrying one, two, or three functional alleles, were generated within three years, and compared to YF47 they all showed improved resistance to naturally inoculated rice blast. Further improved lines (IL) 1 to 5 (all containing Pita, Pib and Pid2 alleles) were evaluated for yield performance, and when no fungicide was applied all lines except IL-4 showed increased traits compared with those of YF47. IL-5, renamed Yanjing 144 (YJ144), showed yield increases in the Liaoning province regional variety comparison test and superior appearance quality compared to YF47. Our work provides a molecular design strategy for pyramiding multiple beneficial genes to rapidly improve rice blast resistance, yield, and quality using multiple breeding strategies.

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“…Meanwhile, another nine polymorphic SSR and EST-SSR markers reported to be related to other putative protein functions and unknown functions were located on LGs 1, 2, 4 and 5 (Isemura et al ., 2012; Chen et al ., 2015). The number of polymorphic SSR and EST-SSR markers obtained from this study may be lower than those in other studies, which have reported using 26–241 polymorphic loci for background selection (Divya et al ., 2014; Miah et al ., 2015; Pradhan et al ., 2015; Ahmed et al ., 2016; Arunakumari et al ., 2016; Xiao et al ., 2016; Krishna et al ., 2017; Yadawad et al ., 2017; Baliyan et al ., 2018; Jamaloddin et al ., 2020; Kumar et al ., 2021). These results may be due to the number of markers and genetic variation of the parents used.…”

Section: Discussionmentioning

confidence: 99%

Improvement of Cercospora leaf spot and powdery mildew resistance of mungbean variety KING through marker-assisted selection

et al. 2021

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The development of resistant mungbean varieties is one of the most efficient strategies to control major diseases such as Cercospora leaf spot (CLS) and powdery mildew (PM). The objectives of this study were to pyramid a CLS resistance gene and two PM resistance genes from the donor parent D2 into a susceptible variety KING through marker-assisted backcrossing (MABC) and to evaluate their agronomic traits and disease resistance under field conditions. Five markers linked to the resistance genes were used for foreground selection, while two marker sets [Set A containing 15 polymorphic simple sequence repeat (SSR) and expressed sequence tag-SSR (EST-SSR) markers and Set B containing 34 polymorphic inter-simple sequence repeat (ISSR) loci] were also used for background selection. Two pyramided backcross (BC) lines, namely H3 and H4, were homozygous at all five marker loci when confirmed in BC4F4 and BC4F5 generations. Their recurrent parent genome (RPG) recovery ranged from 96.4 to 100.0%, depending on the marker sets. During field evaluation, a moderate to high level of CLS and PM resistance was observed in both BC lines compared to the susceptible recurrent parent KING. One of these BC lines (H3) had all agronomic traits similar or superior to the recurrent parent KING at all environments, and had a higher yield than KING (18.0–32.0%) under CLS and PM outbreaks. This line can be developed into a new resistant mungbean variety in Thailand in the future. These results substantiate the usefulness of MABC for transferring multiple resistance genes into an elite variety.

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Integrating marker-assisted background analysis with foreground selection for pyramiding bacterial blight resistance genes into Basmati rice

Cited by 25 publications

References 33 publications

Superior japonica rice variety YJ144 with improved rice blast resistance, yield, and quality achieved using molecular design and multiple breeding strategies

Superior japonica rice variety YJ144 with improved rice blast resistance, yield, and quality achieved using molecular design and multiple breeding strategies

Superior japonica rice variety YJ144 with improved rice blast resistance, yield, and quality achieved using molecular design and multiple breeding strategies

Improvement of Cercospora leaf spot and powdery mildew resistance of mungbean variety KING through marker-assisted selection

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