Integrating high-throughput sequencing and metagenome analysis to reveal the characteristic and resistance mechanism of microbial community in metal contaminated sediments

Yan, Changchun; Wang, Fei; Geng, Huanhuan; Liu, Haijun; Pu, Shengyan; ZhiJun, Tian; Chen, Huilun; Zhou, Beihai; Yuan, Rongfang; Yao, Jun

doi:10.1016/j.scitotenv.2019.136116

Cited by 101 publications

(20 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The reduction or absence of essential microorganisms in the nitrogen cycle will affect the nitrogen cycle chain and the transformation and removal of nitrogenous elements during bioremediation of eutrophic waters 54 . Other studies have shown that heavy metal contamination affects the abundance, composition and structure of microbial communities and disrupts replication processes by destroying microbial DNA 55 , 56 . The abundance of heavy metal susceptible microbes decreases as those resistant to these metal contaminants adapt and proliferate under chemical stress 49 .…”

Section: Discussionmentioning

confidence: 99%

Microbial diversity in intensively farmed lake sediment contaminated by heavy metals and identification of microbial taxa bioindicators of environmental quality

Custodio

Espinoza

Peñaloza

et al. 2022

Sci Rep

View full text Add to dashboard Cite

The cumulative effects of anthropogenic stress on freshwater ecosystems are becoming increasingly evident and worrisome. In lake sediments contaminated by heavy metals, the composition and structure of microbial communities can change and affect nutrient transformation and biogeochemical cycling of sediments. In this study, bacterial and archaeal communities of lake sediments under fish pressure contaminated with heavy metals were investigated by the Illumina MiSeq platform. Despite the similar content of most of the heavy metals in the lagoon sediments, we found that their microbial communities were different in diversity and composition. This difference would be determined by the resilience or tolerance of the microbial communities to the heavy metal enrichment gradient. Thirty-two different phyla and 66 different microbial classes were identified in sediment from the three lagoons studied. The highest percentages of contribution in the differentiation of microbial communities were presented by the classes Alphaproteobacteria (19.08%), Cyanophyceae (14.96%), Betaproteobacteria (9.01%) y Actinobacteria (7.55%). The bacteria that predominated in sediments with high levels of Cd and As were Deltaproteobacteria, Actinobacteria, Coriobacteriia, Nitrososphaeria and Acidobacteria (Pomacocha), Alphaproteobacteria, Chitinophagia, Nitrospira and Clostridia (Tipicocha) and Betaproteobacteria (Tranca Grande). Finally, the results allow us to expand the current knowledge of microbial diversity in lake sediments contaminated with heavy metals and to identify bioindicators taxa of environmental quality that can be used in the monitoring and control of heavy metal contamination.

show abstract

Section: Discussionmentioning

confidence: 99%

Microbial diversity in intensively farmed lake sediment contaminated by heavy metals and identification of microbial taxa bioindicators of environmental quality

Custodio

Espinoza

Peñaloza

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Moreover, other studies have shown that Proteobacteria dominate in the metal-tolerant cultures, because many species belonging to this phyla can reduce Fe and facilitate soil-adsorbed metals release which inadvertently increase metal stress (Francis and Dodge, 1990;Jiang et al, 2017). Proteobacteria and Bacteroidetes have also been reported to harbor the majority of HMs resistance genes base on metagenome sequencing (Yan et al, 2020). Furthermore, an increase in Bacteroidetes relative abundance have been observed in Pb spiked soils compared to the unspiked soils (Hou et al, 2020).…”

Section: Changes In Microbial Community Structurementioning

confidence: 99%

Spatial Variation in Microbial Community in Response to As and Pb Contamination in Paddy Soils Near a Pb-Zn Mining Site

Zou¹,

Lu²,

Dai³

et al. 2021

Front. Environ. Sci.

View full text Add to dashboard Cite

Mining activity is a growing environmental concern as it contributes to heavy metals (HMs) pollution in agricultural soils. Microbial communities play an important role in the biogeochemical cycling of HMs and have the potential to be used as bioindicators. Arsenic (As) and lead (Pb) are the most hazardous HMs and are mainly originated from mining activities. However, spatial variation in microbial community in response to As and Pb contamination in paddy soils remains overlooked. In this study, the biological and chemical properties of sixteen soil samples from four sites (N01, N02, N03, and N04) near a Pb-Zn mining site at different As and Pb levels were examined. The results showed that soil pH, total As and Pb, bioavailable As and Pb, nitrate-nitrogen (NO3−-N) and ammonia-nitrogen (NH4+-N) were the most important factors in shaping the bacterial community structure. In addition, significant correlations between various bacterial genera and As and Pb concentrations were observed, indicating their potential roles in As and Pb biogeochemical cycling. These findings provide insights into the variation of paddy soil bacterial community in soils co-contaminated with different levels of As and Pb.

show abstract

“…The results obtained from the long-term heavy metal/metalloid-polluted sites are consistent with results obtained in independent studies in similar smelter areas (Li et al, 2017(Li et al, , 2020bSchneider et al, 2017;Bai et al, 2020). According to reports, the phylum Proteobacteria contain most of the heavy metal-resistant genes, followed by Actinobacteria and Bacteroidetes, as revealed by metagenome sequencing (Yan et al, 2020). This may explain high relative abundances of Proteobacteria in samples analyzed in this study.…”

Section: Effects Of Metal/metalloid Pollution On the Function Of Microbial Communities In Soilssupporting

confidence: 92%

Data_Sheet_1.docx

View full text Add to dashboard Cite

Integrating high-throughput sequencing and metagenome analysis to reveal the characteristic and resistance mechanism of microbial community in metal contaminated sediments

Cited by 101 publications

References 62 publications

Microbial diversity in intensively farmed lake sediment contaminated by heavy metals and identification of microbial taxa bioindicators of environmental quality

Microbial diversity in intensively farmed lake sediment contaminated by heavy metals and identification of microbial taxa bioindicators of environmental quality

Spatial Variation in Microbial Community in Response to As and Pb Contamination in Paddy Soils Near a Pb-Zn Mining Site

Data_Sheet_1.docx

Contact Info

Product

Resources

About