Integrating high-throughput sequencing and metabolomics to investigate the stereoselective responses of soil microorganisms to chiral fungicide cis-epoxiconazole

Xue, Pengfei; Liu, Xiaowei; Zhao, Liuqing; Zhang, Jingran; He, Zhimin

doi:10.1016/j.chemosphere.2022.134198

Cited by 10 publications

(3 citation statements)

References 58 publications

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“…The composition of these rhizosphere microbial communities varies depending on root exudates, soil factors, and plant types (Lima et al, 2015;Lu et al, 2018). Roots also attract, prevent or kill rhizosphere microbes by root exudates and shape specific rhizosphere microbial communities (Qu et al, 2021;Xue et al, 2022). For example, leguminous plants exude specific signal molecules such as flavonoids, to attract nitrogen-fixing bacteria, and the malic acid and citric acid in tomato root exudates have been demonstrated to attract Pseudomonas fluorescens strains (Weert et al, 2002;Broughton et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Leymus chinensis resists degraded soil stress by modulating root exudate components to attract beneficial microorganisms

Lin

Mei²,

Wei³

et al. 2022

Front. Microbiol.

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Phytoremediation is an effective means to improve degraded soil nutrients and soil structure. Here, we investigated the remediation effects of Leymus chinensis on the physicochemical properties and structure of degraded soil after 3 years of cultivation and explored the bacterial and fungal drivers in root exudates by metabolomics and high-throughput sequencing. The results showed that root exudates increased soil organic matter (SOM), total nitrogen (TN), total phosphorus (TP) and soil aggregates, and organic acids in root exudates reduced pH and activated insoluble nutrients into forms that are available to plants, such as available nitrogen (NH4+-N), nitrate nitrogen (NO3−-N), and available phosphorus (AP). The cultivation of L. chinensis restored the diversity and richness of soil microorganisms and recruited potential beneficial bacteria and fungi to resist degraded soil stress, and L. chinensis also regulated the abundances of organic acids, amino acids and fatty acids in root exudates to remediate degraded soils. Spearman correlation analysis indicated that glutaric acid, 3-hydroxybutyric acid and 4-methylcatechol in root exudates attracted Haliangium, Nitrospira and Mortierella to the rhizosphere and dispersed the relative abundance of the harmful microorganisms Fusicolla and Fusarium. Our results demonstrate that L. chinensis enhances soil fertility, improves soil structure, promotes microbial diversity and abundance, and recruits potentially beneficial microorganisms by modulating root exudate components.

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Section: Introductionmentioning

confidence: 99%

Leymus chinensis resists degraded soil stress by modulating root exudate components to attract beneficial microorganisms

Lin

Mei²,

Wei³

et al. 2022

Front. Microbiol.

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“…Castringnano et al studied the enantiomeric composition of ofloxacin in aquatic environments, revealing enrichment of S-(−)-ofloxacin in the receiving waters of a wastewater treatment plant (WWTP), indicating potential stereoselectivity degradation of ofloxacin by bacteria within the WWTP (Liu et al, 2012). Xue et al reported that the xenobiotic pollutant epoxiconazole altered the structure and metabolism of soil microorganisms with significant stereoselectivity, primarily driven by 2R, 3S-(+)-cisepoxiconazole (Xue et al, 2022).…”

Section: Open Accessmentioning

confidence: 99%

Synergistic antibacterial effects of closantel and its enantiomers in combination with colistin against multidrug resistant gram-negative bacteria

Ding,

Guo,

Fang

et al. 2024

Front. Microbiol.

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Drug combinations and repurposing have recently provided promising alternatives to cope with the increasingly severe issue of antibiotic resistance and depletion of natural drug molecular repertoires that undermine traditional antibacterial strategies. Closantel, an effective adjuvant, reverses antibiotic resistance in gram-negative bacteria. Herein, the combined antibacterial enantioselectivity of closantel is presented through separate enantiomer studies. Despite yielding unexpected differences, two closantel enantiomers (R, S) increased colistin activity against gram-negative bacteria both in vitro and in vivo. The fractional inhibitory concentration indices of R-closantel and S-closantel combined with colistin against Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli ranged from 0.0087 to 0.5004 and from 0.0117 to 0.5312, respectively. This difference was further demonstrated using growth inhibition assays and time-killing curves. Mechanistically, a higher intracellular concentration of R-CLO is more effective in enhancing the antimicrobial activity of combination. A mouse cutaneous infection model confirmed the synergistic stereoselectivity of closantel. This discovery provides novel insights for developing precision medication and containment of increasing antibiotic resistance.

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“…Soil, the main natural resource that supports human survival and development (Xue et al, 2022), is very likely to be contaminated by ILs (Cheng et al, 2019). The microbial communities are important organisms in the soil and they perform important ecological functions, while being sensitive to pollutants (Liu et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Effects of different ionic liquids on microbial growth, enzyme activity and microbial community structure of soil

Chu,

Zheng,

Zhao

et al. 2023

Preprint

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Ionic liquids (ILs) are widely used “green solvent” as they have a low vapor pressure and can replace volatile solvents in industry. However, ILs are difficult to biodegrade and are potentially harmful to the environment. This study, herein, investigated the toxicity of three imidazole ILs ([C8MIM]Cl, [C8MIM]Br, and [C8DMIM]Br) towards soil microorganisms. The results showed that the ILs inhibited the growth of soil culturable microorganisms (fungi, bacteria, and actinomycetes) and affected the activity of soil enzyme. In addition, microbial community species and abundance in soil were altered, with significant differences between the control and experimental groups. Alpha diversity analysis showed that the community abundance tended to increase and then decrease with increasing ILs concentrations. The decrease in species abundance implied that the soil microbial system was less resilient to disturbance. Finally, functional prediction analysis revealed that ILs mainly affected the carbohydrate metabolism and amino acid metabolic processes of the microorganisms. ILs with single methyl substituent had a more pronounced effect than those with double methyl substituents. This study contributes to a better understanding of the environmental safety and ecological risks of ILs.

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Integrating high-throughput sequencing and metabolomics to investigate the stereoselective responses of soil microorganisms to chiral fungicide cis-epoxiconazole

Cited by 10 publications

References 58 publications

Leymus chinensis resists degraded soil stress by modulating root exudate components to attract beneficial microorganisms

Leymus chinensis resists degraded soil stress by modulating root exudate components to attract beneficial microorganisms

Synergistic antibacterial effects of closantel and its enantiomers in combination with colistin against multidrug resistant gram-negative bacteria

Effects of different ionic liquids on microbial growth, enzyme activity and microbial community structure of soil

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