Integrating genetics and morphometrics in species conservation—A case study on the stone crayfish, Austropotamobius torrentium

Berger, Christian; Štambuk, Anamaria; Maguire, Ivana; Weiss, Steven; Füreder, Leopold

doi:10.1016/j.limno.2017.11.002

Cited by 12 publications

(12 citation statements)

References 69 publications

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“…For both mitochondrial genes, ranges of genetic distances between and within the phylogroups (Additional file 3 ) were in accordance with previously reported in Trontelj et al [ 3 ], Klobučar et al [ 4 ], Petrusek et al [ 27 ] and Berger et al [ 46 ]. Some of the observed ranges of the COI genetic distances between phylogroups were within the range of genetic distances found between Astacus species [ 3 , 47 ], Austropotamobius species [ 3 , 48 ] or Australian Parastacidae crayfish [ 49 ].…”

Section: Discussionsupporting

confidence: 90%

“…Geographically and genetically isolated phylogroups represent the evolutionary legacy of A. torrentium which is highly relevant for conservation due to their mostly small distribution ranges and evolutionary distinctness. Since human mediated translocation and restocking of crayfish for repopulation are encouraged with the aim of increasing the genetic diversity of endangered populations [ 115 ], future conservation programs should consider conducting translocations and repopulations only within the same ESU [ 46 , 116 – 118 ].…”

Section: Discussionmentioning

confidence: 99%

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New insights into the genetic diversity of the stone crayfish: taxonomic and conservation implications

et al. 2020

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Background Austropotamobius torrentium is a freshwater crayfish species native to central and south-eastern Europe, with an intricate evolutionary history and the highest genetic diversity recorded in the northern-central Dinarides (NCD). Its populations are facing declines, both in number and size across its entire range. By extanding current knowledge on the genetic diversity of this species, we aim to assist conservation programmes. Multigene phylogenetic analyses were performed using different divergence time estimates based on mitochondrial and, for the first time, nuclear DNA markers on the largest data set analysed so far. In order to reassess taxonomic relationships within this species we applied several species delimitation methods and studied the meristic characters with the intention of finding features that would clearly separate stone crayfish belonging to different phylogroups. Results Our results confirmed the existence of high genetic diversity within A. torrentium, maintained in divergent phylogroups which have their own evolutionary dynamics. A new phylogroup in the Kordun region belonging to NCD has also been discovered. Due to the incongruence between implemented species delimitation approaches and the lack of any morphological characters conserved within lineages, we are of the opinion that phylogroups recovered on mitochondrial and nuclear DNA are cryptic subspecies and distinct evolutionary significant units. Conclusions Geographically and genetically isolated phylogroups represent the evolutionary legacy of A. torrentium and are highly relevant for conservation due to their evolutionary distinctiveness and restricted distribution.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

New insights into the genetic diversity of the stone crayfish: taxonomic and conservation implications

et al. 2020

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“…Different colour dots represent different phylogroups: green dots À Banovina (BAN), black dots À central and southeastern Europe (CSE), blue dots À Gorski Kotar (GK), purple dots À Lika and Dalmatia (LD), orange dots À Žumberak, Plitvice and Bjelolasica (ŽPB). 2012; Perry et al, 2013;Rudolph et al, 2016) or genetic background (Sint et al, 2007;Cataudella et al, 2010;Maguire et al, 2017;Pârvulescu, 2019) or probably combination of both (Baric et al, 2005a(Baric et al, , 2005bBertocchi et al et al, 2008;Mathews et al, 2008;Helms et al, 2015;Berger et al, 2017). Former molecular phylogenetic and phylogeographic research of the stone crayfish based on the mtDNA revealed existence of at least seven (Klobučar et al, 2013) or eight (Pârvulescu et al, 2019) highly divergent monophyletic phylogroups within A. torrentium, with the highest genetic diversity recorded in the north-central Dinarids in Croatia.…”

Section: Discussionmentioning

confidence: 99%

“…Further, all of the measured morphometric characteristics were normalised by dividing their value with the corresponding postorbital length (POL = HEL þ ARL) to avoid comparison of different sized crayfish that could lead to false results (Chambers et al, 1979;Palma and Andrade, 2002;Sint et al, 2005). Since t-test showed that males and females differed significantly in characteristics describing claws and abdomen, the two sexes were analysed separately (Sint et al, 2007;Berger et al, 2017;Maguire et al, 2017;Vlach and Valdmanová, 2015). Observed differences are consequence of sexual dimorphism that is characteristic of crayfish and appears upon crayfish attein sexual maturity (Grandjean et al, 1997;Streissl and Hödl, 2002;Vlach and Valdmanová, 2015).…”

Section: Traditional Morphometric Analysesmentioning

confidence: 99%

Morphological diversity of the stone crayfish − traditional and geometric morphometric approach

Lovrenčić

Pavić

Majnarić

et al. 2019

Knowl. Manag. Aquat. Ecosyst.

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Austropotamobius torrentium is one of four native European crayfish species inhabiting Croatian freshwaters. Existence of eight divergent monophyletic mtDNA phylogroups was described within A. torrentium; six of them are distributed in Croatia, with the highest genetic diversity established in its northern-central Dinaric region. Recent small-scale study of the stone crayfish morphological variability indicated significant differences among different phylogroups. In the present study larger sample size, covering populations from five phylogroups, was analysed with the aim of determining whether there are morphological characteristics that reliably separate stone crayfish from different phylogroups. Aiming this, 245 stone crayfish were analysed through traditional (TM) and, for the first time, geometric morphometric (GM) analyses. Multivariate discriminant analyses included 24 TM characteristics per crayfish, while GM comprised analyses of 22 landmarks on the dorsal side of cephalon. Both methods revealed congruent results, and significant differences among phylogroups in analysed features were obtained, with the cephalon shape contributing the most to crayfish discrimination. Research confirmed that both approaches, combined with statistical methods, are useful in distinguishing and separating crayfish phylogroups. Findings of present study are compatible with the previous molecular findings; stone crayfish present several distinct evolutionary lineages whose species status are currently undefined and require urgent clarification.

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“…Phylogeographic studies serve as a valuable tool in conservation biology [1][2][3][4], not only to help understand the evolutionary history of a species but for providing insight into a species' genetic diversity, which further enables proper assessment of the impact of various threats [5,6]. The use of mitochondrial DNA (mtDNA) has been applied in molecular analyses to aid conservation studies to investigate the genetic diversity and genetic structure of a species and identify any evolutionarily significant units [7][8][9][10][11][12]. Furthermore, phylogeographic studies are used to understand the role of paleogeographical events and demographic processes in shaping diversification within a species and the geographical distribution of the lineages [13].…”

Section: Introductionmentioning

confidence: 99%

Contribution to the phylogeography of the nose-horned viper (Vipera ammodytes, Linnaeus, 1758) in the Central Balkan Peninsula

Čubrić

Stamenković

Ilić³

et al. 2019

ARCH BIOL SCI BELGRA

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Seven genetic clades have been recognized within the species Vipera ammodytes (nose-horned viper); however, the precise phylogenetic position of many Balkan populations is unknown. We used Bayesian analysis of the mtDNA sequences from the 16S rRNA mtDNA gene obtained from 47 individuals (26 novel samples sequenced in this study and 21 sequences available from GenBank). Our results show that sampled nose-horned vipers from localities in Serbia are clustered within three clades: the northeastern (23 individuals), the northwestern (two individuals) and the southeastern (one individual). Results revealed an overlapping distribution of the northeastern and the northwestern clades in two populations. We have revealed that the northeastern clade extends further south than previously suggested, to the Ohrid/Prespa lakes in North Macedonia. Our findings contribute to the knowledge of the genetic diversity of this species in Serbia and help to clarify the geographical distributions of mtDNA-defined clades.

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Integrating genetics and morphometrics in species conservation—A case study on the stone crayfish, Austropotamobius torrentium

Cited by 12 publications

References 69 publications

New insights into the genetic diversity of the stone crayfish: taxonomic and conservation implications

New insights into the genetic diversity of the stone crayfish: taxonomic and conservation implications

Morphological diversity of the stone crayfish − traditional and geometric morphometric approach

Contribution to the phylogeography of the nose-horned viper (Vipera ammodytes, Linnaeus, 1758) in the Central Balkan Peninsula

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