Integrating dye-intercalated DNA dendrimers with electrospun nanofibers: a new fluorescent sensing platform for nucleic acids, proteins, and cells

Wang, Huaming; Tang, Wei; Wei, Hejia; Zhao, Yan; Hu, Shichao; Guan, Yan; Pan, Wei; Xia, Bin; Li, Na; Feng, Lian

doi:10.1039/c5tb00357a

Cited by 24 publications

(12 citation statements)

References 52 publications

(54 reference statements)

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“…This complex structure makes them to exhibit versatile molecular conformations. Dendrimers have attracted a great deal of attention 1 – 4 not only because of their controllable stepwise synthesis, but also because of their potential applications as catalysts 5 – 11 , molecular micelles 12 – 16 , light-harvesting molecules 17 – 22 , and sensors 23 – 28 .…”

Section: Introductionmentioning

confidence: 99%

Design of a Peripheral Building Block for H-Bonded Dendritic Frameworks and Analysis of the Void Space in the Bulk Dendrimers

Lee

Soldatov

Tzeng

et al. 2017

Sci Rep

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Three dendrimers, (t-Bu-G 2 N)2, CC(t-Bu-G 1 N)3 and (t-Bu-G 1 N)2, with 3,5-di-tert-butyl amidobenzene as a common peripheral moiety were prepared in 64–83% yields and characterized. The bulk solids had high BET surface areas of 136–138 m2/g, which were similar for the three dendrimers in spite of their different molecular weight (ranging from 1791 to 2890). It was concluded that the peripheral amide groups do not imbed in the interstitial space of neighbouring dendrimer molecules but rather build a supramolecular architecture through strong intermolecular H-bonds. This mode of assembly generates voids in the bulk dendrimers responsible for sorption properties. The X-ray crystal structure analysis of a compound representing the peripheral moiety of the dendrimers and the FT-IR and powder-XRD data for (t-Bu-G 1 N)2 suggest the proposed supramolecular structure. The isosteric heats of CO2 sorption (Q st) for (t-Bu-G 2 N)2 were significantly higher than those for the other two dendrimers, which is consistent with the formation of a different type of voids within the interstitial space of the molecule. It is suggested that the interstitial void space can be designed and tuned to adjust its properties to a particular task, such as the separation of gases or a catalytic reaction facilitated by the dendrimer.

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Section: Introductionmentioning

confidence: 99%

Design of a Peripheral Building Block for H-Bonded Dendritic Frameworks and Analysis of the Void Space in the Bulk Dendrimers

Lee

Soldatov

Tzeng

et al. 2017

Sci Rep

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“…A novel dye‐intercalated DNA dendrimer (G3SG) 128 was integrated with electrospun nanofibers (NFs) in order to create an amplified fluorescent sensing platform for the detection of nucleic acids, proteins, and cancer cells . SYBR Green I (SG) 129 ( Figure ), is an intercalating dye that specifically binds with the DNA double strands to generate fluorescence .…”

Section: Dyes As Structural Probes Of Dendrimersmentioning

confidence: 99%

“…Fluorescence spectra and photographs (inset) of G3SG, FAM, and PBS, the concentrations of both probes are 20 n m (right). Reproduced with permission . Copyright 2015, The Royal Society of Chemistry.…”

Section: Dyes As Structural Probes Of Dendrimersmentioning

confidence: 99%

Advances in Light‐Emitting Dendrimers

Abd‐El‐Aziz

Abdelghani

Wagner

et al. 2018

Macromol. Rapid Commun.

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with increasing generation), or even at the branching sites. Such dendrimeric structures with incorporated chromophores provide a mimic of the photosynthetic light-harvesting system, in which antenna chromophores surround the inner reaction center. [35][36][37][38] Each photoactive group within a dendrimer can show quite different emission and energy-transfer properties compared with those of the same chromophore within a small molecule (or as a separate free molecule itself), as a result of the close spatial proximity to neighboring moieties. For instance, in well-designed dendrimers, photoexcited chromophores can undergo energy transfer to other units, funneling the energy through the antenna framework toward the core, which can serve as the basis for solar energy conversion, [39,40] luminescent sensors, [41,42] and photoinduced electron transfer (PET) between units of the dendrimers. [43][44][45] This process can also result in the useful formation of excimer and exciplex species within the dendrimer, which will exhibit vastly different emission wavelengths. [46][47][48] Furthermore, dendrimers which contain metal ions have been generated to combine the redox properties of metals with other dendrimeric properties. [27,[49][50][51][52][53][54] In this review we will focus on light-harvesting dendrimeric materials with many illustrative examples. DendrimersThe design of dendrimers with various chromophores has attracted significant attention in light of the dual effect of the luminescence of the chromophores and the morphology of the synthesized dendrimers. Recent developments in this field stem from their wide potential applications, including organic light-emitting diodes, photonic switches and upconversion lasers, as well as sensors and electronic devices. The focus of this comprehensive review is on the design and properties of various classes of lightharvesting dendrimeric materials.

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“…Several unique properties associated with dendrimers allows them to be used in many applications such as catalysis [ 11 , 12 , 13 , 14 ], sensing [ 15 , 16 , 17 ], and fluorescence applications [ 18 , 19 , 20 , 21 , 22 ], General synthesis routes to these dendrimers involve functionalizing the periphery with antimicrobial agents [ 6 ]. The search for unique synthesis approaches of antimicrobial dendrimers is expanding with the focus on enhanced activity upon resistant strains [ 15 , 16 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 ]. The advantage of including η 6 -aryl-η 5 -cyclopentadienyliron (II) complexes into dendritic structures is to provide highly-ordered structures with engaging bioactivity and other properties [ 16 , 37 , 38 ].…”

Section: Introductionmentioning

confidence: 99%

“…Several unique properties associated with dendrimers allows them to be used in many applications such as catalysis [11][12][13][14], sensing [15][16][17], and fluorescence applications [18][19][20][21][22], General synthesis routes to these dendrimers involve functionalizing the periphery with antimicrobial agents [6]. The search for unique synthesis approaches of antimicrobial dendrimers is expanding with the focus on enhanced activity upon resistant strains [15,16,[23][24][25][26][27][28][29][30][31][32][33][34][35][36].…”

Section: Introductionmentioning

confidence: 99%

Design of Organoiron Dendrimers Containing Paracetamol for Enhanced Antibacterial Efficacy

2020

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Paracetamol (acetaminophen) is a common painkiller and antipyretic drug used globally. Attachment of paracetamol to a series of organoiron dendrimers was successfully synthesized. The aim of this study is to combine the benefits of the presence of these redox-active organoiron dendrimers, their antimicrobial activities against some human pathogenic Gram-positive, and the therapeutic characteristics of paracetamol. The antimicrobial activity of these dendrimers was investigated and tested with a minimum inhibitory concentration and this has been reported. Some of these newly synthesized dendrimers exhibited the highest inhibitory activity against methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus faecium (VRE), and Staphylococcus warneri compared to reference drugs. The results of this study indicate that the antimicrobial efficacy of the dendrimers is dependent on the size of the redox-active organoiron dendrimer and its terminal functionalities. The best result has been recorded for the fourth-generation dendrimer 11, which attached to 48 paracetamol end groups and has 90 units composed of the η6-aryl-η5-cyclopentadienyliron (II) complex. This dendrimer presented inhibition of 50% of the growth (IC50) of 0.52 μM for MRSA, 1.02 μM for VRE, and 0.73 μM for Staphylococcus warneri. The structures of the dendrimers were characterized by elemental analysis, Fourier transform infrared (FT-IR), nuclear magnetic resonance (1H-NMR), and 13C-NMR spectroscopic techniques. In addition, all synthesized dendrimers displayed good thermal stability in the range of 300–350 °C following the degradation of the cationic iron moieties which occurred around 200 °C.

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Integrating dye-intercalated DNA dendrimers with electrospun nanofibers: a new fluorescent sensing platform for nucleic acids, proteins, and cells

Abstract: A fluorescent dye-intercalated DNA dendrimer probe was integrated with electrospun nanofibers to create an amplified sensing platform for disease-related species.

Cited by 24 publications

References 52 publications

Design of a Peripheral Building Block for H-Bonded Dendritic Frameworks and Analysis of the Void Space in the Bulk Dendrimers

Design of a Peripheral Building Block for H-Bonded Dendritic Frameworks and Analysis of the Void Space in the Bulk Dendrimers

Advances in Light‐Emitting Dendrimers

Design of Organoiron Dendrimers Containing Paracetamol for Enhanced Antibacterial Efficacy

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