Integrating a Microwave Resonator and a Microchannel with an Immunochromatographic Strip for Stable and Quantitative Biodetection

Abstract

An immunochromatographic strip is an effective diagnostic tool in various fields because of its simplicity, rapidity, and cost-effectiveness. However, typical strips for preliminary screening provide only qualitative or semiquantitative results, and common solutions for quantitative detection by incorporating different kinds of nanoparticles as biomarkers still do not solve this problem thoroughly. Here, we try to tackle this challenge by integrating low-cost membrane-compatible square split-ring resonators and structure-design-flexible microchannels with flexible strips. We experimentally demonstrate that the limit of detection (LOD) and sensitivity of the strip for quantitative detection of Staphylococcus aureus reach 0.784 ng/mL and 10.214 MHz/(ng/mL), respectively. The LOD level is about 63 times higher than that of the color-based strip determined by the naked eye, and the stability is about 18 times higher than that of the fluorescent strip. This work could not only provide a powerful diagnosis tool for the quantitative detection of S. aureus or other molecules but also deliver new avenues for achieving electric field detection of biomolecules, system-level integration of biosensors, and the development of portable diagnostic devices.

Advanced DNA Detection via Multispectral Plasmonic Metasurfaces

“…The minimum concentration of correlated DNA fragments detected in our experiment is 50 fM, which is well below the value (0.87 nM) determined by standard and innovative methods ( Liu et al, 2014 ; Fakih et al, 2017 ; Zhou et al, 2018 , 2019a,b ; Zhang et al, 2019 ). The absolute amount of DNA molecules detected for this last concentration is 1 amol spread on a die area of 1 cm 2 , corresponding to an actual amount on the detection area of 100 × 100 μm 2 in the zepto molar range.…”

Multifunctional Chemical Sensing Platform Based on Dual‐Resonant Infrared Plasmonic Perfect Absorber for On‐Chip Detection of Poly(ethyl cyanoacrylate)

“…Clearly, the presence of PECA caused a redshift of the resonance of the MPA, which was attributed to the dielectric interference of PECA on the plasmonic resonance. [ 38 ] Furthermore, the vibrations at 1747.5 cm −1 (C = O) and 1252.8 cm −1 (C—O—C) of PECA caused obvious changes in the lineshape of the spectrum and were well matched with λ X and λ L resonant mode, respectively. To quantitatively analyze the change of the lineshape, the Lorentz model was applied to calculate the baseline of the spectrum, which was then used as a reference for transformation to extract the enhanced spectra of PECA vibrations.…”

Reagent free detection of SARS-CoV-2 using an antibody-based microwave sensor in a microfluidic platform

“…For example, Stelson et al 29 reported a microwave microfluidic device for label-free detection of conformational changes in a DNA nanomechanical tweezer structure. Zhou et al 30 demonstrated a proof-of-concept method for detecting biomolecules ( i.e. Staphylococcus aureus ) with a largely improved LOD by integrating an immunochromatographic strip and a microwave resonator with a lateral flow assay device.…”