Integrated Whole-Cell Biocatalysis for Trehalose Production from Maltose Using Permeabilized Pseudomonas monteilii Cells and Bioremoval of Byproduct

Abstract

Trehalose is a non-conventional sugar with potent applications in the food, healthcare and biopharma industries. In this study, trehalose was synthesized from maltose using whole-cell Pseudomonas monteilii TBRC 1196 producing trehalose synthase (TreS) as the biocatalyst. The reaction condition was optimized using 1% Triton X-100 permeabilized cells. According to our central composite design (CCD) experiment, the optimal process was achieved at 35 o C and pH 8.0 for 24 h, resulting in the maximum trehalose yield of 51.60 g/g after 12 h using an initial cell loading of 94 g/l. Scale-up production in a lab-scale bioreactor led to the final trehalose concentration of 51.91 g/l with a yield of 51.60 g/g and productivity of 4.37 g/l/h together with 8.24 g/l glucose as a byproduct. A one-pot process integrating trehalose production and byproduct bioremoval showed 53.35% trehalose yield from 107.4 g/l after 15 h by permeabilized P. moteilii cells. The residual maltose and glucose were subsequently removed by Saccharomyces cerevisiae TBRC 12153, resulting in trehalose recovery of 99.23% with 24.85 g/l ethanol obtained as a co-product. The present work provides an integrated alternative process for trehalose production from maltose syrup in bio-industry.

DNA marker utilization for the sustainable production of trehalose

“…In addition, the large-scale production needs to be performed in a bioreactor. This needs an optimization in the processes, such as integrating the fermentation and biocatalyst processes or uses an integrated setting of biocatalyst and bio-removal processes [14,15]. To achieve a sustainable large-scale production of trehalose, discovering TreS enzyme with the superior attributes becomes urgent.…”

Trehalose Production Using Three Extracellular Enzymes Produced via One-Step Fermentation of an Engineered Bacillus subtilis Strain

“…5.4.99.15) and maltooligosyltrehalose trehalohydrolase (MTHase, E.C. 3.2.1.141) for the production of trehalose from maltodextrins or starch, as shown in a schematic diagram Figure S3 [ 9 , 10 , 11 , 12 ]. Currently, trehalose synthase (TreS, E.C.…”

“…Currently, trehalose synthase (TreS, E.C. 5.4.99.16) is used to produce trehalose from maltose [ 9 , 13 ]. The greatest advantage of this one-step process, through the intermolecular rearrangement mechanism, is simplicity [ 9 , 14 ].…”

“…Several recombinant TreS genes have been cloned from different bacterial strains and heterologously expressed in different hosts [ 15 , 16 , 17 , 18 , 19 ]. However, until now, the low expression level of TreS and the relatively high cost of the maltose substrate were the main reasons for making this approach economically unattractive [ 9 ]. The two-enzyme method is still the mainstream technology for industrial trehalose production.…”

“…16) is used to produce trehalose from maltose [9,13]. The greatest advantage of this one-step process, through the intermolecular rearrangement mechanism, is simplicity [9,14]. Most studies on trehalose production via TreS have focused on screening and modifying TreS.…”