Integrated vector management.

Cameron, M. M.; Bell, Michael; Howard, Ashleigh; Lorenz, Lena M.

doi:10.1079/9781845939861.0175

Cited by 8 publications

(10 citation statements)

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“…The implication of interventions that achieve limited sanitation coverage is unclear, based on the previous randomised controlled sanitation trials measuring diarrhoeal outcome . Four of five previous trials did not find any effect of sanitation coverage on diarrhoeal morbidity, even with the largest leap from 35% to 63% coverage achieved in the study considered the best available evidence thus far .…”

Section: Introductionmentioning

confidence: 99%

Exposure–response relationship of neighbourhood sanitation and children's diarrhoea

Jung

Lou

Cheng

2017

Tropical Med Int Health

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Section: Introductionmentioning

confidence: 99%

Exposure–response relationship of neighbourhood sanitation and children's diarrhoea

Jung

Lou

Cheng

2017

Tropical Med Int Health

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“…The control of Ae. aegypti expansion consists in the environmental management by the elimination of domestic and peri-domestic larvae breeding sites and the use of larvicides such as insect grow regulators, organophosphate neurotoxics and the biolarvicide Bacillus thuringiensis israelensis [7]. The use of adulticides is recommended to be restricted to arbovirus epidemic periods [7]; for this, the neurotoxic pyrethroids are the preferred compounds given their favorable toxicological properties.…”

Section: Introductionmentioning

confidence: 99%

First report on kdr mutations in wild populations of Aedes aegypti from Argentina determined by a novel multiplex high resolution melting PCR method

Illanes

Micieli

Shimabukuro

et al. 2023

Preprint

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Background: Aedes aegypti is an urban mosquito vector of dengue and other arboviruses. During epidemic periods, pyrethroid insecticides are used for the control of adult mosquitoes; the worldwide distributed resistance to these insecticides is a cause of failures in vector control campaigns. The primary target of pyrethroids is the voltage-gated sodium channel; point mutations on this channel, called kdr mutations, are associated with pyrethroid resistance. Two kdr mutations, called V1016I and F1534C, augmented in frequency in natural populations of Ae. aegypti from the Americas in the last decade; their association with pyrethroid resistance has been largely demonstrated in field populations throughout the country, and in in vitro assays. The diagnostic of kdr polymorphisms allows an early detection of insecticide resistance spreading, which is critical for timely decisions on vector management. Given the relevance of resistance management, high-throughput methods for kdr genotyping are valuable tools for resistance monitoring programs. These methods should also be cost-effective, to allow regional-scale surveys. Despite the extended presence of Ae. aegypti and the incidence of dengue in Argentina, the presence, abundance and distribution of kdr mutations were not reported in this country up to date. Methods: Aedes aegypti samples were collected from Metropolitan Area of Buenos Aires, and northern localities of Tartagal (Salta province) and Calilegua (Jujuy province). A high resolution melting assay was developed for the simultaneous detection of V1016I and F1534C kdr mutations. Results: We demonstrate the presence of kdrmutations in Argentina in regions under different selection pressure with pyrethroids. We also report a multiplex high-throughput assay based in high resolution melting PCR for the simultaneous genotyping of V1016I and F1534C kdr mutations. Conclusions: We report for the first time the presence of kdr mutations in distant populations from Argentina, with different epidemiological situations and different history of mosquito control efforts. We have developed a high-throughput method for the genotyping of kdr mutations in Ae. aegypti from the American continent. Given its convenient cost and running time, this method can be incorporated in control campaigns for monitoring the presence and spreading of kdr alleles.

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“…aegypti expansion consists in the environmental management by the elimination of domestic and peri-domestic larvae breeding sites and the use of larvicides such as insect grow regulators, organophosphate neurotoxics and the biolarvicide Bacilus thuringiensis israelensis [7]. The use of adulticides is recommended to be restricted to arbovirus epidemic periods [7]; for this, the neurotoxic pyrethroids are the preferred compounds given their favorable toxicological properties. Even though the organophosphate malathion is used in cases of high pyrethroid resistance in other countries, its domi-sanitary application in Argentina is banned [8].…”

Section: Introductionmentioning

confidence: 99%

Multiplex high resolution melting PCR for simultaneous genotyping of pyrethroid-resistance associated mutations in Aedes aegypti. First report on kdr mutations in wild populations from Argentina

Illanes

Micieli

Shimabukuro

et al. 2022

Preprint

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Background: Aedes aegypti is an urban mosquito vector of Dengue and other arboviruses. During epidemic periods, pyrethroid insecticides are used for the control of adult mosquitoes; the worldwide distributed resistance to these insecticides is a cause of failures in vector control campaigns. The primary target of pyrethroids is the voltage-gated sodium channel; point mutations on this channel, called kdr mutations, are associated with pyrethroid resistance. Two kdr mutations, called V1016I and F1534C, augmented in frequency in natural populations of Ae. aegypti from the Americas in the last decade. The diagnostic of kdr polymorphisms allows an early detection of insecticide resistance spreading, which is critical for timely decisions on vector management. Given the relevance of resistance management, high-throughput methods for kdr genotyping are invaluable tools for resistance monitoring programs. These methods should also be cost-effective, to allow regional-scale surveys. Despite the extended presence of Ae. aegypti and the incidence of dengue in Argentina, the presence, abundance and distribution of kdr mutations were not reported in this country up to date. Methodology and findings: We report a multiplex high-throughput assay based in High Resolution Melting PCR for the simultaneous genotyping of 1016 and 1534 sites in voltage-gated sodium channel gene. We used this method for the study of individual mosquito samples collected in localities which received different selection pressure with pyrethroids. Compared to other genotyping methods, multiplex High Resolution Melting was high-throughput, cost-efficient, sensitive and specific. We demonstrate for the first time the presence of kdr mutations in Argentina in regions under different selection pressure with pyrethroids. Conclusions and Significance: We have developed a high-throughput method for the genotyping of alleles associated with pyrethroid resistance in Ae. aegypti from the American continent. The method developed here is comparable in its sensitivity and reliability with other genotyping methods, but reduces costs and running time. It could be incorporated in control campaigns for control the presence and spreading of resistance-associated alleles. We report here for the first time the presence of kdr mutations in distant populations from Argentina, with different epidemiological situations and different history of mosquito control efforts.

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Integrated vector management.

Abstract: This chapter provides an overview on the concept of integrated vector management, as well as describes its application in the control of medically-important vectors (i.e., Anopheles and Stegomyia mosquitoes) and vector-borne diseases (i.e., malaria, lymphatic filariasis, diarrhoea).

Cited by 8 publications

References 0 publications

Exposure–response relationship of neighbourhood sanitation and children's diarrhoea

Exposure–response relationship of neighbourhood sanitation and children's diarrhoea

First report on kdr mutations in wild populations of Aedes aegypti from Argentina determined by a novel multiplex high resolution melting PCR method

Multiplex high resolution melting PCR for simultaneous genotyping of pyrethroid-resistance associated mutations in Aedes aegypti. First report on kdr mutations in wild populations from Argentina

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