“Weedy” red rice is a problematic weed due to its phenotypic similarities with cultivated rice. Limited herbicide availability has driven a need for non-chemical control options for managing this pest. One pre-planting strategy that is being explored is the stale seedbed methodology which aims to maximize soil seedbank withdrawals via germination. This technique is adapted in rice by flooding a field, waiting for germination and emergence of weed seedlings, and completed with a mechanical or chemical control application. Optimization of this process is dependent on maximizing weed seed germination which is primarily influenced by both temperature and moisture availability. Germinability across a range of these factors is not well understood in California red rice. Thus, this study was aimed to determine germinability of California red rice accessions under various temperature and water potential treatments. Previously described red rice accessions 1, 2, 3, and 5 along with M206, a common California rice cultivar, were exposed to temperatures from 10 to 40 C in combination with water potentials of 0, -0.2, -0.4, or -0.8 MPa until either germination or weed seed decay occurred. Statistical analysis indicated a 3-way interaction between accession, temperature, and water potential. Germination reached 95% when seeds were exposed to temperatures from 20 to 35 C in combination with 0 or -0.2 MPa. Germination was lowest when seeds were water stress (-0.8 MPa), and when temperatures were colder than 20°C or warmer than 35°C. M206 cultivar was utilized for comparison and demonstrated cold tolerance by germinating at 10 C while weedy accessions 1, 2, and 3 did not. When temperatures were at or above 15 C however, M206 germinated less often compared with all weedy accessions. Historical pre-plant temperatures in this region align with those required for weedy rice germination, thus, the stale seedbed methodology is a viable strategy in years when ample flood water is available.