Integrated transcriptomic and metabolomic profiles reveal anthocyanin accumulation in Scutellaria baicalensis petal coloration

Hu, Suying; Wang, Wentao; Zhang, Caijuan; Zhou, Wen; Yan, Pengdong; Xue, Xiaoshan; Tian, Qian; Wang, Donghao; Niu, Junfeng; Wang, Shiqiang; Qiang, Yi; Bai, Chen; Cui, Langjun; Cao, Xiaoyan; Wang, Zhezhi

doi:10.1016/j.indcrop.2022.116144

Cited by 7 publications

(5 citation statements)

References 38 publications

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“…Scutellaria baicalensis , a widely used medicinal plant, has had its active ingredient biosynthesis pathway largely resolved in recent years with the development of genome and transcriptome sequencing technologies [ 45 , 46 ]. The heterologous synthesis of some important components has been achieved through synthetic biology methods, providing more alternative pathways for acquiring active ingredients [ 25 , 27 , 28 ].…”

Section: Discussionmentioning

confidence: 99%

Multi-omics landscape to decrypt the distinct flavonoid biosynthesis of Scutellaria baicalensis across multiple tissues

Guo,

Zhu,

Wang

et al. 2023

Horticulture Research

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Scutellaria baicalensis Georgi, also known as Huang-Qin in Traditional Chinese Medicine, is a widely used herbal remedy due to its anticancer, antivirus, and hepatoprotective properties. The S. baicalensis genome was sequenced many years ago, by contrast, the proteome as the executer of most biological processes of S. baicalensis in the aerial parts, as well as the secondary structure of the roots (xylem, phloem and periderm) is far less comprehensively characterized. Here, we attempt to depict the molecular landscape of the non-model plant S. baicalensis through a multi-omics approach, with the goal of constructing a highly informative and valuable reference dataset. Furthermore, we provide an in-depth characterization dissection to explain two distinct flavonoid biosynthesis pathways existed in aerial part and root, respectively, at the protein and phosphorylated protein level. Our study provides detailed spatial proteomic and phosphoproteomic information in the context of secondary structures, with implications for the molecular profiling of secondary metabolite biosynthesis in non-model medicinal plants.

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Section: Discussionmentioning

confidence: 99%

Multi-omics landscape to decrypt the distinct flavonoid biosynthesis of Scutellaria baicalensis across multiple tissues

Guo,

Zhu,

Wang

et al. 2023

Horticulture Research

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“…These results reveal that anthocyanins might play crucial roles in the color formation of purple flowers. The compound class heatmap of anthocyanins indicates that a total of 71 differentially accumulated metabolites (DAMs) were identified from the three petal samples and classified into eight categories, including cyanidin (12), delphinidin (11), malvidin (9), pelargonidin (13), peonidin (8), petunidin (6), procyanidin (4) and flavonoid (8) (Figure 2B,C). Moreover, the statistics on the number of differentially accumulated metabolites show that 33 upregulated and 9 downregulated, 15 upregulated and 20 downregulated, and 38 upregulated and 13 downregulated DAMs were identified in the purple flowers vs. cream flowers (P vs. C), the yellow flowers vs. cream flowers (Y vs. C) and the purple flowers vs. yellow flowers (P vs. Y), respectively (Table 1).…”

Section: Targeted Anthocyanin Metabolite Assays Of Flowers Of Three D...mentioning

confidence: 99%

“…Plants’ flower color formation is mainly affected by three pigments, including flavonoids, carotenoids and betaines [ 7 ]. Anthocyanins are a class of flavonoids and the principal pigments that confer the leaves, petals, peels and seed coats of plants with purple/red/blue/pink colors [ 8 , 9 ]. In addition, anthocyanins can help plants cope with abiotic stress and improve human immunity to prevent diseases [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Integrated Metabolomic and Transcriptomic Profiles Provide Insights into the Mechanisms of Anthocyanin and Carotenoid Biosynthesis in Petals of Medicago sativa ssp. sativa and Medicago sativa ssp. falcata

Huang,

Liu,

Qiang

et al. 2024

Plants

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The petals of Medicago sativa ssp. sativa and M. sativa ssp. falcata are purple and yellow, respectively. Free hybridization between M. sativa ssp. sativa and M. sativa ssp. falcata has created hybrids with various flower colors in nature. Moreover, the flower colors of alfalfa are closely correlated with yield, nutritional quality, stress tolerance and other agronomic characteristics. To elucidate the underlying mechanisms of flower color formation in M. sativa ssp. sativa and M. sativa ssp. falcata, we conducted an integrative analysis of the transcriptome and metabolome of alfalfa with three different petal colors (purple, yellow and cream). The metabolic profiles suggested that anthocyanins and carotenoids are the crucial pigments in purple and yellow flowers, respectively. A quantitative exploration of the anthocyanin and carotenoid components indicated that the accumulations of cyanidin, delphinidin, peonidin, malvidin, pelargonidin and petunidin derivatives are significantly higher in purple flowers than in cream flowers. In addition, the content of carotenes (phytoene, α-carotene and β-carotene) and xanthophylls (α-cryptoxanthin, lutein, β-cryptoxanthin, zeaxanthin, antheraxanthin and violaxanthin derivatives) was markedly higher in yellow flowers than in cream flowers. Furthermore, we found that delphinidin-3,5-O-diglucoside and lutein were the predominant pigments accumulated in purple and yellow flowers, respectively. The transcriptomic results revealed that twenty-five upregulated structural genes (one C4H, three 4CL, twelve CHS, two CHI, one F3H, one F3′H, one F3′5′H and four DFR) are involved in the accumulation of anthocyanins in purple flowers, and nine structural genes (two PSY, one ZDS, two CRTISO, two BCH, one ZEP and one ECH) exert an effect on the carotenoid biosynthesis pathway in yellow flowers. The findings of this study reveal the underlying mechanisms of anthocyanin and carotenoid biosynthesis in alfalfa with three classic flower colors.

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“…Metabolites are believed to be the real drivers in the ultimate stress responses of plants, especially secondary metabolites. For example, a recent study has shown that anthocyanin accumulation altered the petal coloration of Scutellaria baicalensis [15]. Several free amino acids and secondary metabolites, including flavonoids and phenylpropanoids, have been identified as regulators of plant homeostasis under environmental stress conditions [16,17].…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptomics and Metabolomics Analyses of Avicennia marina and Kandelia obovata under Chilling Stress during Seedling Stage

Wang,

Cheng

2023

IJMS

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One of the most productive ecosystems in the world, mangroves are susceptible to cold stress. However, there is currently insufficient knowledge of the adaptation mechanisms of mangrove plants in response to chilling stress. This study conducted a comparative analysis of transcriptomics and metabolomics to investigate the adaptive responses of Kandelia obovata (chilling-tolerant) and Avicennia marina (chilling-sensitive) to 5 °C. The transcriptomics results revealed that differentially expressed genes (DEGs) were mostly enriched in signal transduction, photosynthesis-related pathways, and phenylpropanoid biosynthesis. The expression pattern of genes involved in photosynthesis-related pathways in A. marina presented a downregulation of most DEGs, which correlated with the decrease in total chlorophyll content. In the susceptible A. marina, all DEGs encoding mitogen-activated protein kinase were upregulated. Phenylpropanoid-related genes were observed to be highly induced in K. obovata. Additionally, several metabolites, such as 4-aminobutyric acid, exhibited higher levels in K. obovata than in A. marina, suggesting that chilling-tolerant varieties regulated more metabolites in response to chilling. The investigation defined the inherent distinctions between K. obovata and A. marina in terms of signal transduction gene expression, as well as phenylpropanoid and flavonoid biosynthesis, during exposure to low temperatures.

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Integrated transcriptomic and metabolomic profiles reveal anthocyanin accumulation in Scutellaria baicalensis petal coloration

Cited by 7 publications

References 38 publications

Multi-omics landscape to decrypt the distinct flavonoid biosynthesis of Scutellaria baicalensis across multiple tissues

Multi-omics landscape to decrypt the distinct flavonoid biosynthesis of Scutellaria baicalensis across multiple tissues

Integrated Metabolomic and Transcriptomic Profiles Provide Insights into the Mechanisms of Anthocyanin and Carotenoid Biosynthesis in Petals of Medicago sativa ssp. sativa and Medicago sativa ssp. falcata

Comparative Transcriptomics and Metabolomics Analyses of Avicennia marina and Kandelia obovata under Chilling Stress during Seedling Stage

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