Integrated transcriptomic and metabolomic analyses of Caucasian clover (Trifolium ambiguum Bieb.) in response to freezing stress

Mou, Dan; Li, Zongren; Liu, Wenhui; Liang, Guoling; Jia, Zhifeng; Yu, Hongyan; Wang, Jiuluan; Ou, Weiyou; Li, Kai; Yao, Xixi; Zhan, Yu; Ma, Geliang; Kazhuo, Cairang; Li, Xilai; Xie, Jun

doi:10.1007/s40415-022-00786-4

Cited by 3 publications

(1 citation statement)

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“…It has a welldeveloped root system, and the main root is clearly expanded and penetrates deep into the soil; it can grow into new plants through the rhizome and has a high regeneration capacity [3,4]. Studies have also shown that Caucasian clover is more resistant to cold and heat, has better drought resistance and is also more resistant to disease than other similar plants [5][6][7][8][9][10]. However, the low seed yield and weak seedling vigor of Caucasian clover make direct sowing for grassland restoration very difficult.…”

Section: Introductionmentioning

confidence: 99%

Identification and Evolutionary Analysis of the Auxin Response Factor (ARF) Family Based on Transcriptome Data from Caucasian Clover and Analysis of Expression Responses to Hormones

Jiang,

Wang,

Chen

et al. 2023

IJMS

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Caucasian clover (Trifolium ambiguum M. Bieb.) is an excellent perennial plant in the legume family Fabaceae, with a well-developed rhizome and strong clonal growth. Auxin is one of the most important phytohormones in plants and plays an important role in plant growth and development. Auxin response factor (ARF) can regulate the expression of auxin-responsive genes, thus participating in multiple pathways of auxin transduction signaling in a synergistic manner. No genomic database has been established for Caucasian clover. In this study, 71 TaARF genes were identified through a transcriptomic database of Caucasian clover rhizome development. Phylogenetic analysis grouped the TaARFs into six (1–6) clades. Thirty TaARFs contained a complete ARF structure, including three relatively conserved regions. Physical and chemical property analysis revealed that TaARFs are unstable and hydrophilic proteins. We also analyzed the expression pattern of TaARFs in different tissues (taproot, horizontal rhizome, swelling of taproot, rhizome bud and rhizome bud tip). Quantitative real–time RT–PCR revealed that all TaARFs were responsive to phytohormones (indole-3-acetic acid, gibberellic acid, abscisic acid and methyl jasmonate) in roots, stems and leaves. These results helped elucidate the role of ARFs in responses to different hormone treatments in Caucasian clover.

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