Integrated transcriptomic and metabolic analysis response in gills, hepatopancreas, and muscle metabolism in oriental river prawn Macrobrachium nipponense in response to acute high salinity stress

Fan, Yaoran; Feng, Jianbin; Wang, Zefei; Ling, Feiyue; Knepper, M. A.; Hua, Xueming; Li, Juan-Ying; Li, Jiale

doi:10.1016/j.aqrep.2022.101358

Cited by 6 publications

(6 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In aquaculture, salinity is one of the most critical environmental factors affecting the establishment, development, and growth of aquatic organisms, as well as their distribution and physiological functions (Fan et al, 2022a;Fan et al, 2022b). Crustacea are sensitive to salinity, adversely affecting their metabolism, osmoregulation, and growth traits.…”

Section: Introductionmentioning

confidence: 99%

“…The oriental river prawn, Macrobrachium nipponense, is one of the most critical commercial aquaculture species in Europe, China, Japan, and Southeast Asia (Fan et al, 2022a;Fan et al, 2022b). M. nipponense commonly occurs in estuaries and freshwater habitats with low salinity (Ma et al, 2011;Ma et al, 2012;Kumar Chourasia et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, the nutrition performance (Ding et al, 2017), germplasm regulation (Ma et al, 2012), and immune resource (Tang et al, 2017) of M. nipponense were also essentially deliberative processes. Even though there has been considerable progress in crustacean research over the last few decades, only a few studies have explicitly focused on salinity adaptations (Fan et al, 2022a). However, it is remarkable how differently M. nipponense responds to long-term salinity gradients at the transcriptomic and proteomic levels.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, it is essential to understand the mechanisms underlying salinity adaptation in M. nipponese, transcriptome and proteome were conducted. A wide variety of applications have been made possible by transcriptomes to reveal molecular resources for non-model organisms with biological and economic importance at the gene level (Dai et al, 2021;Fan et al, 2022a;Xue et al, 2022). By contrast, the proteomic analysis reveals how signal transduction impacts the biochemical process (Jin et al, 2018;Chang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Transcriptome, Proteome, Histology, and Biochemistry Analysis of Oriental River Prawn Macrobrachium nipponense under Long-term Salinity Exposure

Fan

Wu²,

Ling³

et al. 2023

Front. Mar. Sci.

Self Cite

View full text Add to dashboard Cite

Salinity is an ecological factor affecting the physiology, survival, and distribution of crustaceans. Additionally, salinity fluctuation detrimentally affects the composition and biological process of crustaceans. As a significant commercial aquaculture species in China, Japan, and Southeast Asian countries, the oriental river prawn, Macrobrachium nipponense, can tolerate a wide range of salinity. The transcriptome, proteome, histology, and physiology analysis were utilized to explore the physiological responses and molecular mechanisms of salinity tolerance in M. nipponense. Through the three-month culture, the statistic of growth trait illustrated the relatively excellent performance of M. nipponense in low salinity, and the higher salinity exposure significantly affected the growth of M. nipponense. In terms of the histological analysis, the gills and hepatopancreas of M. nipponense suffered varying degrees of damage. Besides, the activities of the digestive, immune-related, and metabolic enzymes were calculated. These results indicated that salinity significantly influenced trypsin and amylase in hepatopancreas, especially in 14 ppt. The immune-related enzymes were activated in high salinity. Notably, the activity of metabolic enzymes was significantly low in 7 and 14 ppt, which testified that the 7 ppt to 14 ppt were near the isotonic point of M. nipponense. In gills, hepatopancreas, and muscle, high-throughput mRNA sequencing revealed 11356, 2227, and 1819 differentially expressed genes (DEGs) by comparing the 7, 14, and 21 ppt groups with the 0ppt group, respectively. The TMT-labeling proteome identified 439 and 230 differentially expressed proteins (DEPs) in gills and hepatopancreas through the comparison of the 7, 14, and 21 ppt groups to the 0 ppt group, respectively. Additionally, through the integration of transcriptome and proteome, several pathways related to salinity adaptation were enriched, including protein export, cGMP-PKG signaling pathway, Amino sugar and nucleotide sugar metabolism, and Glycine, serine and threonine metabolism. Besides, 16 up and down-regulated proteins and related DEGs were detected through KEGG enrichment analysis, including ETHE1, BIP, chitinase (E3.2.1.14), and SARDH. Notably, no significantly regulated proteins and related DEGs were recorded by the correlation of transcriptome and proteome of 0 ppt and 7 ppt in hepatopancreas. Thus, the optimum survival salinity of M. nipponense may range from 0 ppt to 7 ppt. Overall, these results may provide valuable insights into the mechanisms underlying the culture of M. nipponense in different salinity.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Transcriptome, Proteome, Histology, and Biochemistry Analysis of Oriental River Prawn Macrobrachium nipponense under Long-term Salinity Exposure

Fan

Wu²,

Ling³

et al. 2023

Front. Mar. Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The oriental river prawn, Macrobrachium nipponense, has become an economic aquaculture species in China, Japan, and Vietnam. (Fan et al 2022b). Studies have illustrated that the optimal growth water temperature for M. nipponense is 25 ℃ (Wang et al 2006), and its oxidative stress is minimized at this temperature.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic, histological and biochemical analyses of Macrobrachium nipponense response to acute heat stress

Fan

Knepper

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Temperature is an essential factor affecting the viability of crustaceans, and high temperature can cause damage or even death. The oriental river prawn, Macrobrachium nipponense, is an important economic aquaculture species in China, Japan, and Vietnam. To identify the transcriptomic, histological, and biochemical response of M. nipponense and reveal their adaptation mechanisms, the prawns were placed at 25 ℃, 30 ℃, and 35 ℃ for 24 h. The histological damages in the gills and hepatopancreas of M. nipponense were found under acute heat stress. Additionally, acute heat stress enhanced the digestive, metabolic, and antioxidative capacity of M. nipponense by biochemical analysis. The total RNA of hepatopancreas and gills were isolated and sequenced using the RNA-Seq method. After filtration, assembly, and aggregation, a total of 131690 unigenes were identified. Gene ontology (GO) analysis revealed that differentially expressed genes (DEGs) were significantly involved in the regulation of transcription by RNA polymerase II, proteolysis, nucleus, cytoplasm, nucleus, and ATP binding. In the hepatopancreas, several pathways were significantly enriched in the treatment groups, including neuroactive ligand-receptor interaction, thyroid hormone synthesis, and ECM-receptor interaction. And in the gills, cGMP-PKG signaling pathway, ribosome, and calcium signaling pathway, were enriched. The transcriptomic analysis provided insights into the thermoregulation and molecular mechanisms of M. nipponense in response to acute heat stress.

show abstract

Temporal Transcriptomic Profiling Reveals Dynamic Changes in Gene Expression of Giant Freshwater Prawn upon Acute Saline-Alkaline Stresses

Chen,

Zhu,

Feng

et al. 2024

Mar Biotechnol

View full text Add to dashboard Cite

Integrated transcriptomic and metabolic analysis response in gills, hepatopancreas, and muscle metabolism in oriental river prawn Macrobrachium nipponense in response to acute high salinity stress

Cited by 6 publications

References 46 publications

Transcriptome, Proteome, Histology, and Biochemistry Analysis of Oriental River Prawn Macrobrachium nipponense under Long-term Salinity Exposure

Transcriptome, Proteome, Histology, and Biochemistry Analysis of Oriental River Prawn Macrobrachium nipponense under Long-term Salinity Exposure

Transcriptomic, histological and biochemical analyses of Macrobrachium nipponense response to acute heat stress

Temporal Transcriptomic Profiling Reveals Dynamic Changes in Gene Expression of Giant Freshwater Prawn upon Acute Saline-Alkaline Stresses

Contact Info

Product

Resources

About