Integrated transcriptome-proteome analyses of human stem cells reveal source-dependent differences in their regenerative signature

Ganguly, Abantika; Swaminathan, Ganesh; Garcia-Marques, Fernando Jose; Regmi, Shobha; Yarani, Reza; Primavera, Rosita; Chetty, Shashank; Bermudez, Abel; Pitteri, Sharon J.; Thakor, Avnesh S.

doi:10.1016/j.stemcr.2022.11.006

Cited by 4 publications

(1 citation statement)

References 60 publications

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“…Testing the same panel of antibodies in hMSCs revealed a reduced number of detectable RBPs (13 of 18), with lack of signal for AQR, EFTUD2, LIN28B, ZNF622, and PPIG. These results largely agree with the transcriptomics and proteomics literature in bone-marrow-derived hMSCs 20 that indicates very low or even absent expression of those genes. For the quantifiable RBP candidates, 61.5% (8 of 13) were detected in CM, and 31% (4 of 13) had a higher relative abundance in the extracellular space than in cells, showing a range of enrichment from 2.1–6.3 ( Figures 3 B, 3C, and S3 B).…”

Section: Resultssupporting

confidence: 91%

exRNA-eCLIP intersection analysis reveals a map of extracellular RNA binding proteins and associated RNAs across major human biofluids and carriers

LaPlante¹,

Sturchler²,

Fullem³

et al. 2023

Cell Genomics

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Section: Resultssupporting

confidence: 91%

exRNA-eCLIP intersection analysis reveals a map of extracellular RNA binding proteins and associated RNAs across major human biofluids and carriers

LaPlante¹,

Sturchler²,

Fullem³

et al. 2023

Cell Genomics

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Facing the Challenges in the COVID-19 Pandemic Era: From Standard Treatments to the Umbilical Cord-Derived Mesenchymal Stromal Cells as a New Therapeutic Strategy

Russo

Corrao

Gaudio

et al. 2023

Cells

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Coronavirus disease 2019 (COVID-19), the pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which counts more than 650 million cases and more than 6.6 million of deaths worldwide, affects the respiratory system with typical symptoms such as fever, cough, sore throat, acute respiratory distress syndrome (ARDS), and fatigue. Other nonpulmonary manifestations are related with abnormal inflammatory response, the “cytokine storm”, that could lead to a multiorgan disease and to death. Evolution of effective vaccines against SARS-CoV-2 provided multiple options to prevent the infection, but the treatment of the severe forms remains difficult to manage. The cytokine storm is usually counteracted with standard medical care and anti-inflammatory drugs, but researchers moved forward their studies on new strategies based on cell therapy approaches. The perinatal tissues, such as placental membranes, amniotic fluid, and umbilical cord derivatives, are enriched in mesenchymal stromal cells (MSCs) that exert a well-known anti-inflammatory role, immune response modulation, and tissue repair. In this review, we focused on umbilical-cord-derived MSCs (UC-MSCs) used in in vitro and in vivo studies in order to evaluate the weakening of the severe symptoms, and on recent clinical trials from different databases, supporting the favorable potential of UC-MSCs as therapeutic strategy.

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TMT-Based Quantitative Proteomics Analysis Reveals Differentially Expressed Proteins between Different Sources of hMSCs

Naudot,

Le Ber,

Marcelo

2023

IJMS

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Mesenchymal stem cells (MSCs) are an attractive therapeutic tool for tissue engineering and regenerative medicine owing to their regenerative and trophic properties. The best-known and most widely used are bone marrow MSCs, which are currently being harvested and developed from a wide range of adult and perinatal tissues. MSCs from different sources are believed to have different secretion potentials and production, which may influence their therapeutic effects. To confirm this, we performed a quantitative proteomic analysis based on the TMT technique of MSCs from three different sources: Wharton’s jelly (WJ), dental pulp (DP), and bone marrow (BM). Our analysis focused on MSC biological properties of interest for tissue engineering. We identified a total of 611 differentially expressed human proteins. WJ-MSCs showed the greatest variation compared with the other sources. WJ produced more extracellular matrix (ECM) proteins and ECM-affiliated proteins and proteins related to the inflammatory and immune response processes. BM-MSCs expressed more proteins involved in osteogenic, adipogenic, neuronal, or muscular differentiation and proteins involved in paracrine communication. Compared to the other sources, DP-MSCs overexpressed proteins involved in the exocytosis process. The results obtained confirm the existence of differences between WJ, DP, and BM-MSCs and the need to select the MSC origin according to the therapeutic objective sought.

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Integrated transcriptome-proteome analyses of human stem cells reveal source-dependent differences in their regenerative signature

Cited by 4 publications

References 60 publications

exRNA-eCLIP intersection analysis reveals a map of extracellular RNA binding proteins and associated RNAs across major human biofluids and carriers

exRNA-eCLIP intersection analysis reveals a map of extracellular RNA binding proteins and associated RNAs across major human biofluids and carriers

Facing the Challenges in the COVID-19 Pandemic Era: From Standard Treatments to the Umbilical Cord-Derived Mesenchymal Stromal Cells as a New Therapeutic Strategy

TMT-Based Quantitative Proteomics Analysis Reveals Differentially Expressed Proteins between Different Sources of hMSCs

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