Integrated Transcriptome and Metabolome Analysis Reveals Key Metabolites Involved in Camellia oleifera Defense against Anthracnose

Yang, Chaochen; Wu, Pengfei; Yao, Xiaohua; Sheng, Yu; Zhang, Chengcai; Lin, Peng-Cheng; Wang, Kailiang

doi:10.3390/ijms23010536

Cited by 27 publications

(21 citation statements)

References 74 publications

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“…In addition, in this study, the differentially expressed genes that were significantly enriched in BP functions were related to the secondary metabolic process, the most significantly enriched CC function was cell wall, and the most significantly enriched MF function was chitin binding in the resistant plants compared to the susceptible plants. This was consistent with research results on the defense response of Camellia oleifera against the anthracnose fungal disease [ 33 ]. Altogether, these results suggest that callose synthesis and deposition play an active role in plant resistance to powdery mildew.…”

Section: Discussionsupporting

confidence: 93%

Morphological and Molecular Analyses of the Interaction between Rosa multiflora and Podosphaera pannosa

Bao

Zhang

Sun

et al. 2022

Genes

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Powdery mildew disease caused by Podosphaerapannosa is the most widespread disease in global cut-rose production, as well as a major disease in garden and pot roses. In this study, the powdery mildew resistance of different wild rose varieties was evaluated. Rose varieties with high resistance and high sensitivity were used for cytological observation and transcriptome and expression profile analyses to study changes at the morphological and molecular levels during the interaction between Rosa multiflora and P. pannosa. There were significant differences in powdery mildew resistance among three R. multiflora plants; R. multiflora ‘13’ had high resistance, while R. multiflora ‘4’ and ‘1’ had high susceptibility. Cytological observations showed that in susceptible plants, 96 and 144 h after inoculation, hyphae were observed in infected leaves; hyphae infected the leaf tissue through the stoma of the lower epidermis, while papillae were formed on the upper epidermis of susceptible leaf tissue. Gene ontology enrichment analysis showed that the differentially expressed genes that were significantly enriched in biological process functions were related to the secondary metabolic process, the most significantly enriched cellular component function was cell wall, and the most significantly enriched molecular function was chitin binding. Changes in the transcript levels of important defense-related genes were analyzed. The results showed that chitinase may have played an important role in the interactions between resistant R. multiflora and P. pannosa. Jasmonic acid and ethylene (JA/ET) signaling pathways might be triggered in the interaction between susceptible R. multiflora and P. pannosa. In the resistant R. multiflora, the salicylic acid (SA) signaling pathway was induced earlier. Between susceptible plants and resistant plants, key phenylpropanoid pathway genes were induced and upregulated after P. pannosa inoculation, demonstrating that the phenylpropanoid pathway and secondary metabolites may play important and active roles in R. multiflora defense against powdery mildew infection.

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Section: Discussionsupporting

confidence: 93%

Morphological and Molecular Analyses of the Interaction between Rosa multiflora and Podosphaera pannosa

Bao

Zhang

Sun

et al. 2022

Genes

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“…Sade et al [ 163 ] conducted a study on susceptible and resistant tomato plants to determining the differences in the regulated pathways and the levels of specialized metabolites between these two samples in response to tomato yellow leaf curl virus invasion using both comparative transcriptomics and metabolomics. In another example, transcriptomic and metabolomic analyses were performed by Yang et al, to detect the differentially expressed genes (DEGs) and metabolites of anthracnose-resistant and susceptible varieties of Camellia oleifera , suggesting the important role of flavonoid biosynthesis in the defense against anthracnose [ 164 ]. As mentioned in the report of Kaul et al [ 146 ], differential expression analysis regarding endophyte-free and endophyte-inoculated plants can be helpful to fully understand the mechanisms of plant–endophyte interactions and endophyte-mediated disease resistance.…”

Section: Multi-omics Approaches For Mining Bioactive Metabolites From...mentioning

confidence: 99%

The Multifunctions and Future Prospects of Endophytes and Their Metabolites in Plant Disease Management

et al. 2022

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Endophytes represent a ubiquitous and magical world in plants. Almost all plant species studied by different researchers have been found to harbor one or more endophytes, which protect host plants from pathogen invasion and from adverse environmental conditions. They produce various metabolites that can directly inhibit the growth of pathogens and even promote the growth and development of the host plants. In this review, we focus on the biological control of plant diseases, aiming to elucidate the contribution and key roles of endophytes and their metabolites in this field with the latest research information. Metabolites synthesized by endophytes are part of plant disease management, and the application of endophyte metabolites to induce plant resistance is very promising. Furthermore, multi-omics should be more fully utilized in plant–microbe research, especially in mining novel bioactive metabolites. We believe that the utilization of endophytes and their metabolites for plant disease management is a meaningful and promising research direction that can lead to new breakthroughs in the development of more effective and ecosystem-friendly insecticides and fungicides in modern agriculture.

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“…Despite with long history of conventional breeding, the superior varieties of C. lanceolata with desirable wood quality are still scarce and cannot meet the market demands. At present, the molecular marker-assisted selection provides an effective strategy for breeding programmes of trees ( Plomion et al, 1996 ; Lebedev et al, 2020 ; Yang et al, 2022 ). However, the shortage of genetic information severely limits the molecular-marker-assisted selection of C. lanceolata .…”

Section: Introductionmentioning

confidence: 99%

Full-Length Transcriptome Sequencing and Comparative Transcriptomic Analyses Provide Comprehensive Insight Into Molecular Mechanisms of Cellulose and Lignin Biosynthesis in Cunninghamia lanceolata

Zhuang

Lin

et al. 2022

Front. Plant Sci.

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Cunninghamia lanceolata is an essential timber species that provide 20%–30% raw materials for China’s timber industry. Although a few transcriptomes have been published in C. lanceolata, full-length mRNA transcripts and regulatory mechanisms behind the cellulose and lignin biosynthesis have not been thoroughly investigated. Here, PacBio Iso-seq and RNA-seq analyses were adapted to identify the full-length and differentially expressed transcripts along a developmental gradient from apex to base of C. lanceolata shoots. A total of 48,846 high-quality full-length transcripts were obtained, of which 88.0% are completed transcriptome based on benchmarking universal single-copy orthologs (BUSCO) assessment. Along stem developmental gradient, 18,714 differentially expressed genes (DEGs) were detected. Further, 28 and 125 DEGs were identified as enzyme-coding genes of cellulose and lignin biosynthesis, respectively. Moreover, 57 transcription factors (TFs), including MYB and NAC, were identified to be involved in the regulatory network of cellulose and lignin biosynthesis through weighted gene co-expression network analysis (WGCNA). These TFs are composed of a comparable regulatory network of secondary cell wall formation in angiosperms, revealing a similar mechanism may exist in gymnosperms. Further, through qRT-PCR, we also investigated eight specific TFs involved in compression wood formation. Our findings provide a comprehensive and valuable source for molecular genetics breeding of C. lanceolata and will be beneficial for molecular-assisted selection.

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Integrated Transcriptome and Metabolome Analysis Reveals Key Metabolites Involved in Camellia oleifera Defense against Anthracnose

Cited by 27 publications

References 74 publications

Morphological and Molecular Analyses of the Interaction between Rosa multiflora and Podosphaera pannosa

Morphological and Molecular Analyses of the Interaction between Rosa multiflora and Podosphaera pannosa

The Multifunctions and Future Prospects of Endophytes and Their Metabolites in Plant Disease Management

Full-Length Transcriptome Sequencing and Comparative Transcriptomic Analyses Provide Comprehensive Insight Into Molecular Mechanisms of Cellulose and Lignin Biosynthesis in Cunninghamia lanceolata

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