Integrated Transcriptome and Biochemical Analysis Provides New Insights into the Leaf Color Change in Acer fabri

Li, Guohua; Gu, Heng; Cai, Heng; Guo, Congcong; Chen, Ying; Wang, Lianggui; Chen, Gongwei

doi:10.3390/f14081638

Cited by 4 publications

(4 citation statements)

References 47 publications

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“…With development, the anthocyanin content in the leaves gradually decreased and the carotenoid (lutein and zeaxanthin) and chlorophyll (chlorophyll a and chlorophyll b) contents gradually increased until, finally, the leaves appeared green. The results appear to be similar to previous studies [2].…”

Section: Discussionsupporting

confidence: 92%

“…After transcriptome sequencing the different colors of Acer pseudosieboldianum leaves, 50,501 genes were identified [42]. A total of 53,550 genes were identified by transcriptome sequencing different colors of Acer fabri leaves and three key genes related to anthocyanin synthesis were identified [2]. We conducted transcriptome sequencing for six stages of leaf color changes of O. fragrans 'Wucaigui' and compared the results with the whole genome of O. fragrans.…”

Section: Discussionmentioning

confidence: 99%

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Integrated Metabolome and Transcriptome Analyses Provide New Insights into the Leaf Color Changes in Osmanthus fragrans cv. ‘Wucaigui’

Zhang,

Qiu,

Wang

et al. 2024

Forests

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Osmanthus fragrans, belonging to the family Oleaceae, is listed as one of the most important traditional ornamental plant species in China. A new cultivar O. fragrans ‘Wucaigui’ has a very diversified form in terms of leaf colors, in which the leaf color changes from red to yellow-green and finally to dark green. To understand the mechanisms involved in leaf color changes, metabolome and transcriptome studies were performed on leaves at different developmental stages. A total of 79 metabolites, two chlorophyll, 26 carotenoids, and 51 anthocyanins, were detected in the 6 different developmental stages. An orthogonal partial least squares discriminant analysis identified key metabolites at different developmental stages, including lutein, pelargonidin-3-O-(6-O-p-coumaroyl)-glucoside, neoxanthin, and α-carotene. A total of 48,837 genes were obtained by transcriptome sequencing, including 3295 novel genes. Using a weighted gene co-expression network analysis to study the correlations between key metabolites and differentially expressed genes, we determined the characteristic modules having the highest correlations with key metabolites and selected associated candidate genes. Five genes (OfSHOU4L, OfATL1B, OfUGE5 OfEIF1AX, and OfUGE3) were finally identified as hub genes using real-time fluorescence quantitative PCR. In addition, we proposed a model based on the changes in key metabolite contents and the network regulatory map during the changes in O. fragrans ‘Wucaigui’ leaf color. The positive regulation of OfUGE3 led to an increase in the lutein content, which resulted in the leaves changing from grayish brown to moderate brown; during the change from moderate brown to dark greenish-yellow, the positive regulation of three genes (OfHOU4L, OfATL1B, and OfUGE5) increased the content of pelargonidin-3-O-(6-O-p-coumaroyl)-glucoside and the red color of the leaves gradually faded to dark greenish-yellow and then to strong yellow-green; the positive regulation of OfEIF1AX increased the content of neoxanthin; the stages in which the color changed from strong yellow-green to yellow-green and then to moderate olive-green were positively regulated by OfUGE3, which resulted in higher α-carotene content. These findings provided new insights into the mechanisms underlying the processes involved in O. fragrans ‘Wucaigui’ leaf color changes at the metabolic and transcriptional levels. This work seeks to contribute to the development of artificial regulate and control technology in the breeding and production of O. fragrans and other ornamental plants.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Integrated Metabolome and Transcriptome Analyses Provide New Insights into the Leaf Color Changes in Osmanthus fragrans cv. ‘Wucaigui’

Zhang,

Qiu,

Wang

et al. 2024

Forests

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“…In Arabidopsis, AtNAC087 overexpression induces anthocyanin accumulation, decreases chlorophyll content, and enhances the expression of leaf senescence-related genes. The expression of AfNAC083 is positively correlated with the anthocyanin content in Acer fabri [53]. IbNAC056a/b in sweet potato promotes anthocyanin accumulation [54].…”

Section: Discussionmentioning

confidence: 98%

Integrated transcriptomics and metabolomics analyses provide insights into anthocyanin biosynthesis for leaf colour formation in Quercus mongolica

Yuan,

Liu,

et al. 2024

Preprint

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Quercus mongolica is a tall tree with a broad, rounded crown and lush leaves. In autumn, the leaves turn red and have great ornamental value. However, the molecular mechanisms that cause the change in leaf colour are unknown. In this study, we identified 12 differentially expressed genes involved in anthocyanin synthesis by analysing the transcriptome of Q. mongolica leaves in six developmental stages (S1 − S6). We further analysed the dynamics of anthocyanin content in Q. mongolica leaves in four developmental stages (S1, S2, S5, and S6) using differential gene expression patterns. We detected a total of 48 anthocyanins and categorised these into seven major anthocyanin ligands. The most abundant anthocyanins in the red leaves of Q. mongolica were cyanidin-3,5-O-diglucoside, cyanidin-3-O-glucoside, cyanidin-3-O-sophoroside, and pelargonidin-3-O-glucoside. Correlation analysis of differentially expressed genes and anthocyanin content identified highly expressed QmANS as a key structural gene associated with anthocyanin biosynthesis in Q. mongolica. A transcription factor-structural gene correlation analysis showed that the 1bHLH, 3bZIP, 1MYB, 10NAC, and 2WRKY transcription factors played strong positive roles in regulating anthocyanin structural genes (|PCC| > 0.90), with the QmNAC transcription factor playing a major role in anthocyanin biosynthesis.

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“…Huang et al investigated the expression of circRNAs associated with the senescence process in rice rapeseed leaves and reported that 17 senescence-related circRNAs were predicted to have parental genes by weighted gene co-expression network analysis (WGCNA), of which regulation of their parental genes by three circRNAs was verified by qRT-PCR [ 62 ]. The expression of AfNAC083 in Acer fabri is positively correlated with anthocyanin content [ 63 ]. Our analysis of the DECs and their parental genes showed that the parental gene of novel_circ_003018 is gene-Qm012601 (NAC083), which is an important transcription factor regulating leaf colour in Q. mongolica .…”

Section: Discussionmentioning

confidence: 99%

Identification and Characterisation of the CircRNAs Involved in the Regulation of Leaf Colour in Quercus mongolica

Yuan,

Pang,

Pang

et al. 2024

Biology

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Circular RNAs (circRNAs) are important regulatory molecules involved in various biological processes. However, the potential function of circRNAs in the turning red process of Quercus mongolica leaves is unclear. This study used RNA-seq data to identify 6228 circRNAs in leaf samples from four different developmental stages and showed that 88 circRNAs were differentially expressed. A correlation analysis was performed between anthocyanins and the circRNAs. A total of 16 circRNAs that may be involved in regulating the colour of Mongolian oak leaves were identified. CircRNAs may affect the colour of Q. mongolica leaves by regulating auxin, cytokinin, gibberellin, ethylene, and abscisic acid. This study revealed the potential role of circRNAs in the colour change of Q. mongolica leaves.

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Integrated Transcriptome and Biochemical Analysis Provides New Insights into the Leaf Color Change in Acer fabri

Cited by 4 publications

References 47 publications

Integrated Metabolome and Transcriptome Analyses Provide New Insights into the Leaf Color Changes in Osmanthus fragrans cv. ‘Wucaigui’

Integrated Metabolome and Transcriptome Analyses Provide New Insights into the Leaf Color Changes in Osmanthus fragrans cv. ‘Wucaigui’

Integrated transcriptomics and metabolomics analyses provide insights into anthocyanin biosynthesis for leaf colour formation in Quercus mongolica

Identification and Characterisation of the CircRNAs Involved in the Regulation of Leaf Colour in Quercus mongolica

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