Integrated transcriptome analysis of immune-related mRNAs and microRNAs in Macrobrachium rosenbergii infected with Spiroplasma eriocheiris

Ou, Jiangtao; Chen, Hao; Li, Qiao; Bian, Yunxia; Luan, Xiaoqi; Jiang, Qichen; Ji, Hao; Lv, Lishuang; Dong, Xiaohui; Zhao, Wei; Zhang, Qihuan

doi:10.1016/j.fsi.2021.11.002

Cited by 10 publications

(2 citation statements)

References 57 publications

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“…The Toll receptor is typically composed of transmembrane regions, intracellular interleukin hormone receptor domains, and extracellular leucine repeats [34]. It is the key vector in immune signal transduction [35]. In the present study, the expression of Toll continuously increased in gills for 24 h and remained highly expressed in hemolymph and hepatopancreas at 6 hpi, indicating that the Toll gene is involved in the immune response against A. veronii infection.…”

Section: Important Immune-related Genes Involved In the Immune Responsesupporting

confidence: 54%

The Dynamics of Gene Expression Unraveling the Immune Response of Macrobrachium rosenbergii Infected by Aeromonas veronii

Peng

Liu

Zhong

et al. 2023

Genes

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To further investigate the immune response of Macrobrachium rosenbergii against Aeromonas veronii, comparative transcriptomic analyses of the M. rosenbergii hepatopancreas were conducted on challenge and control groups at 6, 12, and 24 h post-infection (hpi), independently. A total of 51,707 high-quality unigenes were collected from the RNA-seq data, and 8060 differentially expressed genes (DEGs) were discovered through paired comparisons. Among the three comparison groups, a KEGG pathway enrichment analysis showed that 173 immune-related DEGs were considerably clustered into 28 immune-related pathways, including the lysosome, the phagosome, etc. Moreover, the expression levels of the four key immune-related genes (TOLL, PAK1, GSK3β, and IKKα) were evaluated at various stages following post-infection in the hepatopancreas, hemolymph, and gills. Both PAK1 and GSK3β genes were highly up-regulated in all three tissues at 6 hpi with A. veronii; TOLL was up-regulated in the hepatopancreas and hemolymph but down-regulated in the gill at 6 hpi, and IKKα was up-regulated in hemolymph and gill, but down-regulated in the hepatopancreas at 6 hpi. These findings lay the groundwork for understanding the immune mechanism of M. rosenbergii after contracting A. veronii.

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Section: Important Immune-related Genes Involved In the Immune Responsesupporting

confidence: 54%

The Dynamics of Gene Expression Unraveling the Immune Response of Macrobrachium rosenbergii Infected by Aeromonas veronii

Peng

Liu

Zhong

et al. 2023

Genes

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show abstract

“…For instance, the p38/MAPK pathway initiated intestinal innate immunity in Caenorhabditis elegans under Pseudomonas aeruginosa infection [55]. In addition to that, Inada [57]. Among these miRNAs, the target of PC-mro-3p-103, PN-mro-miR-306, and PN-mro-miR-316 are sprouty-related EVH1 domain containing 1 (SPRED1), while PC-mro-5p-62 is a protein inhibitor of activated STAT.…”

Section: Vascular Endothelial Growth Factor (Vegf) Pathwaymentioning

confidence: 99%

MicroRNA Regulation in Infectious Diseases and Its Potential as a Biosensor in Future Aquaculture Industry: A Review

SiouNing,

Seong,

Kondo

et al. 2023

Molecules

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An infectious disease is the most apprehensive problem in aquaculture as it can lead to high mortality in aquatic organisms and massive economic loss. Even though significant progress has been accomplished in therapeutic, prevention, and diagnostic using several potential technologies, more robust inventions and breakthroughs should be achieved to control the spread of infectious diseases. MicroRNA (miRNA) is an endogenous small non-coding RNA that post-transcriptionally regulates the protein-coding genes. It involves various biological regulatory mechanisms in organisms such as cell differentiation, proliferation, immune responses, development, apoptosis, and others. Furthermore, an miRNA also acts as a mediator to either regulate host responses or enhance the replication of diseases during infection. Therefore, the emergence of miRNAs could be potential candidates for the establishment of diagnostic tools for numerous infectious diseases. Interestingly, studies have revealed that miRNAs can be used as biomarkers and biosensors to detect diseases, and can also be used to design vaccines to attenuate pathogens. This review provides an overview of miRNA biogenesis and specifically focuses on its regulation during infection in aquatic organisms, especially on the host immune responses and how miRNAs enhance the replication of pathogens in the organism. In addition to that, we explored the potential applications, including diagnostic methods and treatments, that can be employed in the aquaculture industry.

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Oxidative stress responses of the crayfish Procambrus clarkii to Spiroplasma eriocheiris challenge

Ding

2022

Aquaculture Reports

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Integrated transcriptome analysis of immune-related mRNAs and microRNAs in Macrobrachium rosenbergii infected with Spiroplasma eriocheiris

Cited by 10 publications

References 57 publications

The Dynamics of Gene Expression Unraveling the Immune Response of Macrobrachium rosenbergii Infected by Aeromonas veronii

The Dynamics of Gene Expression Unraveling the Immune Response of Macrobrachium rosenbergii Infected by Aeromonas veronii

MicroRNA Regulation in Infectious Diseases and Its Potential as a Biosensor in Future Aquaculture Industry: A Review

Oxidative stress responses of the crayfish Procambrus clarkii to Spiroplasma eriocheiris challenge

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